pLX304-V5-Blast-Empty

Empty control vector for the ORF collection in the pLX304 lentiviral vector.

Sequence Author: transOMIC

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CMV enhancer pLX304-V5-Blast-Empty 7671 bp
AmpR
2738 .. 3598  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2738 .. 2806  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2738 .. 3598  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2807 .. 3598  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2738 .. 3598  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
WPRE
678 .. 1266  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
678 .. 1266  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
3769 .. 4357  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3769 .. 4357  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hPGK promoter
6535 .. 7035  =  501 bp
human phosphoglycerate kinase 1 promoter
hPGK promoter
6535 .. 7035  =  501 bp
human phosphoglycerate kinase 1 promoter
f1 ori
2152 .. 2607  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
2152 .. 2607  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
BSD
7047 .. 7442  =  396 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
BSD
7047 .. 7442  =  396 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
CMV enhancer
65 .. 368  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
65 .. 368  =  304 bp
human cytomegalovirus immediate early enhancer
3' LTR (ΔU3)
1338 .. 1571  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
3' LTR (ΔU3)
1338 .. 1571  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
RRE
5898 .. 6131  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
5898 .. 6131  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RSV promoter
4826 .. 5052  =  227 bp
Rous sarcoma virus enhancer/promoter
RSV promoter
4826 .. 5052  =  227 bp
Rous sarcoma virus enhancer/promoter
CMV promoter
369 .. 572  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
369 .. 572  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
5' LTR (truncated)
5053 .. 5233  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
5053 .. 5233  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
SV40 ori
1810 .. 1945  =  136 bp
SV40 origin of replication
SV40 ori
1810 .. 1945  =  136 bp
SV40 origin of replication
HIV-1 Ψ
5280 .. 5405  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
5280 .. 5405  =  126 bp
packaging signal of human immunodeficiency virus type 1
SV40 poly(A) signal
1649 .. 1770  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1649 .. 1770  =  122 bp
SV40 polyadenylation signal
cPPT/CTS
7502 .. 7619  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
7502 .. 7619  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
2633 .. 2737  =  105 bp
AmpR promoter
2633 .. 2737  =  105 bp
V5 tag
595 .. 636  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
595 .. 636  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
ORF:  781 .. 1311  =  531 bp
ORF:  176 amino acids  =  18.7 kDa
ORF:  5776 .. 6759  =  984 bp
ORF:  327 amino acids  =  36.7 kDa
ORF:  6817 .. 7266  =  450 bp
ORF:  149 amino acids  =  15.8 kDa
ORF:  2738 .. 3598  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  768 .. 1277  =  510 bp
ORF:  169 amino acids  =  18.5 kDa
ORF:  1671 .. 1907  =  237 bp
ORF:  78 amino acids  =  8.6 kDa
ORF:  4755 .. 5000  =  246 bp
ORF:  81 amino acids  =  8.9 kDa
ORF:  7047 .. 7457  =  411 bp
ORF:  136 amino acids  =  14.2 kDa
ORF:  3202 .. 3468  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5691 .. 6071  =  381 bp
ORF:  126 amino acids  =  13.3 kDa
ORF:  6504 .. 7349  =  846 bp
ORF:  281 amino acids  =  30.3 kDa
ORF:  4682 .. 4981  =  300 bp
ORF:  99 amino acids  =  10.9 kDa
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Download pLX304-V5-Blast-Empty.dna file

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