pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro

|Download SnapGene Viewer
No matches
SgrAI (7424) SspI (6685) XmnI (6480) ScaI (6361) AflIII - PciI (4988) HindIII (4757) AvrII (4741) SfiI (4694) HincII (3669) BbvCI (209) AscI (369) SpeI (661) BglII (1410) BbsI - BpiI (1683) BbsI - BpiI (1709) PaeR7I - PspXI - XhoI (1732) BsgI (1777) EcoRI (1849) AgeI (1858) BstBI (1867) AleI (2284) PflMI (2889) PshAI (2923) BamHI (2995) BsiWI (3117) BspEI * (3174) RsrII (3177) SalI (3667) pMSCV16URP-U6-sh-HTS6- UbiC-TagRFP-2A-Puro 7602 bp
SgrAI  (7424)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SspI  (6685)
1 site
A A T A T T T T A T A A
XmnI  (6480)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (6361)
1 site
A G T A C T T C A T G A
AflIII  (4988)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4988)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
HindIII  (4757)
1 site
A A G C T T T T C G A A
AvrII  (4741)
1 site
C C T A G G G G A T C C
SfiI  (4694)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
HincII  (3669)
1 site
G T Y R A C C A R Y T G
BbvCI  (209)
1 site
C C T C A G C G G A G T C G
AscI  (369)
1 site
G G C G C G C C C C G C G C G G
SpeI  (661)
1 site
A C T A G T T G A T C A
BglII  (1410)
1 site
A G A T C T T C T A G A
BbsI  (1683)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BpiI  (1683)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpiI sites may not be compatible.
BbsI  (1709)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BpiI  (1709)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpiI sites may not be compatible.
PaeR7I  (1732)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1732)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1732)
1 site
C T C G A G G A G C T C
BsgI  (1777)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
EcoRI  (1849)
1 site
G A A T T C C T T A A G
AgeI  (1858)
1 site
A C C G G T T G G C C A
BstBI  (1867)
1 site
T T C G A A A A G C T T
AleI  (2284)
1 site
C A C N N N N G T G G T G N N N N C A C
PflMI  (2889)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
PshAI  (2923)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BamHI  (2995)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BsiWI  (3117)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
BspEI  (3174)
1 site
T C C G G A A G G C C T
* Blocked by Dam methylation.
RsrII  (3177)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
SalI  (3667)
1 site
G T C G A C C A G C T G
TagRFP
2284 .. 2994  =  711 bp
237 amino acids  =  26.7 kDa
Product: monomeric derivative of red fluorescent protein from Entacmaea quadricolor (Merzlyak et al., 2007)
mammalian codon-optimized
TagRFP
2284 .. 2994  =  711 bp
237 amino acids  =  26.7 kDa
Product: monomeric derivative of red fluorescent protein from Entacmaea quadricolor (Merzlyak et al., 2007)
mammalian codon-optimized
T2A
3001 .. 3054  =  54 bp
18 amino acids  =  1.8 kDa
Product: 2A peptide from Thosea asigna virus capsid protein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
T2A
3001 .. 3054  =  54 bp
18 amino acids  =  1.8 kDa
Product: 2A peptide from Thosea asigna virus capsid protein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
PuroR
3061 .. 3660  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
3061 .. 3660  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
AmpR
5808 .. 6668  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5808 .. 6599  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5808 .. 6668  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6600 .. 6668  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5808 .. 6668  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
5049 .. 5637  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5049 .. 5637  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
5' LTR
7601 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem cell virus
5' LTR
7601 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem cell virus
3' LTR (ΔU3)
3736 .. 4161  =  426 bp
self-inactivating 3' long terminal repeat (LTR) from Moloney murine leukemia virus
3' LTR (ΔU3)
3736 .. 4161  =  426 bp
self-inactivating 3' long terminal repeat (LTR) from Moloney murine leukemia virus
gag (truncated)
987 .. 1403  =  417 bp
truncated MMLV gag gene lacking the start codon
gag (truncated)
987 .. 1403  =  417 bp
truncated MMLV gag gene lacking the start codon
UbiC promoter
1872 .. 2271  =  400 bp
human ubiquitin C promoter
UbiC promoter
1872 .. 2271  =  400 bp
human ubiquitin C promoter
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell virus
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell virus
SV40 promoter
4427 .. 4756  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
4427 .. 4756  =  330 bp
SV40 enhancer and early promoter
U6 promoter
1438 .. 1686  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
1438 .. 1686  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
AmpR promoter
6669 .. 6773  =  105 bp
AmpR promoter
6669 .. 6773  =  105 bp
SV40 ori
4607 .. 4742  =  136 bp
SV40 origin of replication
SV40 ori
4607 .. 4742  =  136 bp
SV40 origin of replication
cloning site
1684 .. 1713  =  30 bp
shRNA cloning site
cloning site
1684 .. 1713  =  30 bp
shRNA cloning site
ORF:  2284 .. 3660  =  1377 bp
ORF:  458 amino acids  =  50.3 kDa
ORF:  5938 .. 6204  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  7054 .. 7350  =  297 bp
ORF:  98 amino acids  =  11.7 kDa
ORF:  3985 .. 4275  =  291 bp
ORF:  96 amino acids  =  10.5 kDa
ORF:  2070 .. 2339  =  270 bp
ORF:  89 amino acids  =  10.0 kDa
ORF:  2397 .. 2642  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
ORF:  5808 .. 6668  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  362 .. 637  =  276 bp
ORF:  91 amino acids  =  9.9 kDa
ORF:  674 .. 934  =  261 bp
ORF:  86 amino acids  =  9.5 kDa
ORF:  7118 .. 7348  =  231 bp
ORF:  76 amino acids  =  7.5 kDa
Click here to try SnapGene

Download pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.