Learn Gateway Cloning
This in-depth course examines the Gateway Cloning technique. It includes a detailed overview, pros and cons, top tips and a how-to guide for using Gateway Cloning in SnapGene.
Introduction to Gateway Cloning
INSIDE THE VIDEO
The Gateway cloning method, developed by Invitrogen in the 1990s, uses the site-specific recombination machinery of phage lambda to integrate its genome into E. Coli.
LENGTH
4 Minutes
A Detailed Look at Gateway Cloning
INSIDE THE VIDEO
Take a detailed look at ThermoFisher's proprietary system Gateway cloning. Learn how the 2-step BP and LR reactions work to make Gateway cloning an effective cloning technique.
LENGTH
6 Minutes
Gateway Cloning Pros and Cons
INSIDE THE VIDEO
In this video, we discuss the relative pros and cons of Gateway Cloning. Pros include its speed, high throughput and the ability to combine multiple fragments in one reaction.
LENGTH
4 Minutes
Tips for Gateway Cloning
INSIDE THE VIDEO
Now that you're familiar with the technique of Gateway Cloning take your learning further with these 5 tips for carrying out successful cloning experiments in the lab.
LENGTH
5 Minutes
Recommended Resources
GUIDEGateway Cloning Technique
An overview of Thermo Fisher Scientific's Gateway Cloning technique and how to simulate the Gateway reaction in SnapGene.
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