pQE-T7 (cloning region)

Cloning region of pQE-T7, the parental vector for the QIAgenes system.

Sequence Author: Qiagen

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HomePlasmidsQiagen VectorspQE-T7 (cloning region)
250 200 150 100 50 End (276) EcoO109I (245) StyI (240) BlpI (218) MspA1I (217) EcoP15I (178) BstYI (165) BsiHKAI - Bsp1286I (146) AvaI - XhoI - BsoBI - PspXI - PaeR7I - BmeT110I (139) NdeI (126) NdeI (102) XbaI (62) BsrBI (45) SfcI (28) AseI (15) Start (0) T7 promoter lac operator ATG 6xHis QIAgene start codon stop codons 6xHis T7 terminator pQE-T7 (cloning region) 276 bp
End  (276)
0 sites
EcoO109I  (245)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
StyI  (240)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
BlpI  (218)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
MspA1I  (217)
1 site		 C M G C K G G K C G M C
EcoP15I  (178)
1 site		 C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N
Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BstYI  (165)
1 site		 R G A T C Y Y C T A G R
BsiHKAI  (146)
1 site		 G W G C W C C W C G W G
Sticky ends from different BsiHKAI sites may not be compatible.
Bsp1286I  (146)
1 site		 G D G C H C C H C G D G
Sticky ends from different Bsp1286I sites may not be compatible.
AvaI  (139)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
XhoI  (139)
1 site		 C T C G A G G A G C T C
BsoBI  (139)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PspXI  (139)
1 site		 V C T C G A G B B G A G C T C V
PaeR7I  (139)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
BmeT110I  (139)
1 site		 C Y C G R G G R G C Y C
NdeI  (126)
2 sites		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
NdeI  (102)
2 sites		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
XbaI  (62)
1 site		 T C T A G A A G A T C T
BsrBI  (45)
1 site		 C C G C T C G G C G A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
SfcI  (28)
1 site		 C T R Y A G G A Y R T C
Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AseI  (15)
1 site		 A T T A A T T A A T T A
Start  (0)
0 sites
T7 promoter
16 .. 34  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
16 .. 34  =  19 bp
promoter for bacteriophage T7 RNA polymerase
lac operator
35 .. 59  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
35 .. 59  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
ATG
104 .. 106  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
104 .. 106  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
6xHis
110 .. 127  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
110 .. 127  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
QIAgene start codon
128 .. 130  =  3 bp
1 amino acid  =  149.2 Da
QIAgene start codon
128 .. 130  =  3 bp
1 amino acid  =  149.2 Da
stop codons
131 .. 136  =  6 bp
two tandem stop codons
stop codons
131 .. 136  =  6 bp
two tandem stop codons
6xHis
145 .. 162  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
145 .. 162  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
T7 terminator
229 .. 276  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
229 .. 276  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
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Individual Sequences & Maps

pDrive
pQE-19
pQE-50
pQE-9
pDrive (linearized)
pQE-22
pQE-51
pQE-T7 (cloning region)
pIX 3.0
pQE-3
pQE-52
pQE-T7-1
pIX 4.0
pQE-30
pQE-6
pQE-T7-2
pQE-10
pQE-30 UA
pQE-60
pQE-TriSystem
pQE-100 DoubleTag
pQE-30 UA (linearized)
pQE-7
pQE-TriSystem His-Strep1
pQE-11
pQE-30 Xa
pQE-70
pQE-TriSystem His-Strep2
pQE-12
pQE-31
pQE-8
pQE-TriSystem Strep
pQE-13
pQE-32
pQE-80L
pQE-Trisystem-5
pQE-14
pQE-4
pQE-80L-Kan
pQE-Trisystem-6
pQE-15
pQE-40
pQE-81L
pQE-TriSystem-7
pQE-16
pQE-41
pQE-81L-Kan
pREP4
pQE-17
pQE-42
pQE-82L
TAGZyme pQE-1
pQE-18
pQE-5
pQE-82L-Kan
TAGZyme pQE-2

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