CRISPR Plasmids

CRISPR plasmids for genome editing and gene regulation from Addgene, transOMIC, and others

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HomePlasmidsCRISPR Plasmids

Individual Sequences & Maps

3xFLAG-dCas9 pCMV-7.1

pCRISPRa-Enhancer

pME-Cas9-T2A-GFP

3xFLAG-dCas9 pMXs-neo

3xFLAG-dCas9 pMXs-puro

pcDNA-dCas9-p300 Core

CRISPRi library backbone

pcDNA-dCas9-p300 Core (D1399Y)

pMSCV-LTR-dCas9-p65AD-BFP

pcDNA-dCas9-VP64

pdCas9-bacteria

pMSCV-LTR-dCas9-VP64-BFP

pcDNA3.1-CibN-dCas9-CibN

pdCas9-humanized

GeneArt CRISPR Nuclease Vector - CD4 (linearized)

pCFD1-dU6-1gRNA

pdCas9 BFP-humanized

GeneArt CRISPR Nuclease Vector - OFP (linearized)

pCFD2-dU6-2gRNA

pCFD3-dU6-3gRNA

pDD162 (Peft-3_Cas9 + Empty sgRNA)

pCFD4-U6-1_U6-3tandemgRNAs

gRNA_Cloning Vector

pCLIP-All-EFS-Ametrine

Peft-3_cas9-SV40_NLS_tbb-2 3'UTR

pROSA26-Puro-DNR

pCLIP-All-EFS-Blast

pET-28b-Cas9-His

pRPR1_gRNA_handle_RPR1t

pCLIP-All-EFS-Puro

pCLIP-All-EFS-tRFP

pET-Cas9-NLS-6xHis

pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

pCLIP-All-EFS-ZsGreen

pET-NLS-Cas9-6xHis

pSLQ1658-dCas9-EGFP

pCLIP-All-hCMV-Blast

pET28a Cas9-Cys

pSpCas9(BB)-2A-GFP (PX458)

pCLIP-All-hCMV-Puro

pSpCas9(BB)-2A-Puro (PX459) V2.0

lenti dCAS-VP64_Blast

pCLIP-All-hCMV-tRFP

pGL3-U6-sgRNA-PGK-puromycin

pSpCas9n(BB)-2A-GFP (PX461)

lenti MS2-P65-HSF1_Hygro

pCLIP-All-hCMV-ZsGreen

pSpCas9n(BB)-2A-Puro (PX462) V2.0

lenti sgRNA(MS2)_zeo backbone

pCLIP-All-Nickase(D10A)-EFS-Blast

pgRNA-humanized

pSpCas9n(BB) (PX460)

lentiCas9-Blast

pCLIP-All-Nickase(D10A)-EFS-Puro

pSpCas9 (PX165)

pCLIP-All-Nickase(D10A)-EFS-tRFP

pGuide-it-sgRNA1 Vector (Linear)

lentiCas9n(D10A)-Blast

pCLIP-All-Nickase(D10A)-EFS-ZsGreen

pGuide-it-tdTomato Vector (Linear)

pCLIP-All-Nickase(D10A)-hCMV-Puro

pGuide-it-ZsGreen1 Vector (Linear)

lentiGuide-Puro

pCLIP-All-Nickase(D10A)-hCMV-tRFP

pHAGE EF1-alpha dCas9-KRAB

pST1374-N-NLS-flag-linker-Cas9-D10A

LSL-Cas9-Rosa26TV

pCLIP-All-Nickase(D10A)-hCMV-ZsGreen

pHAGE EF1-alpha dCas9-VP64

pST1374-N-NLS-flag-linker-Cas9-H840A

pCLIP-All-Nickase(D10A)-TRE3G-Blast

pHAGE TRE dCas9

pST1374-NLS-flag-linker-Cas9

pCLIP-All-Nickase(D10A)-TRE3G-Puro

pHAGE TRE dCas9-KRAB

pCLIP-All-Nickase(D10A)-TRE3G-tRFP

pHAGE TRE dCas9-VP64

pCLIP-All-Nickase(D10A)-TRE3G-ZsGreen

pHAGE-TO-dCas9-3XGFP

pT7-Cas9-Nickase

pCLIP-All-TRE3G-Blast

pHAGE-TO-dCas9-3XmCherry

MS2-P65-HSF1_GFP

pCLIP-All-TRE3G-Puro

pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

pCLIP-All-TRE3G-tRFP

pHAGE-TO-nmdCas9-3XGFP

pCLIP-All-TRE3G-ZsGreen

pHAGE-TO-nmdCas9-3XmCherry

pTDH3-dCas9-Mxi1

pCLIP-Cas9(D10a)-Nickase-EFS-Puro

pHR-SFFV-dCas9-BFP

pTOL-hCMV-TET3G-Hygro Transactivator

pCLIP-Cas9(D10a)-Nickase-EFS-Blast

pHR-SFFV-dCas9-BFP-KRAB

pTPGI_dCas9_VP64

pCLIP-Cas9(D10a)-Nickase-EFS-tRFP

pHR-SFFV-KRAB-dCas9-P2A-mCherry

pU6-BbsI-chiRNA

p414-TEF1p-Cas9-CYC1t

pCLIP-Cas9(D10a)-Nickase-EFS-ZsGreen

pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

PU6_klp-12_sgRNA

p415-GalL-Cas9-CYC1t

pCLIP-Cas9(D10a)-Nickase-hCMV-Blast

pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

p426-SNR52p-gRNA.CAN1.Y-SUP4t

pCLIP-Cas9(D10a)-Nickase-hCMV-Puro

pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

pAAV-Guide-it-1 Vector (Linear)

pCLIP-Cas9(D10a)-Nickase-hCMV-tRFP

pAAV-Guide-it-Down Vector (Linear)

pCLIP-Cas9(D10a)-Nickase-hCMV-ZsGreen

pUC57-sgRNA expression vector

pAAV-Guide-it-Up Vector

pCLIP-Cas9(D10a)-Nickase-TRE3G-Blast

pICH41308_hCas9

Puro-Cas9 donor

pCLIP-Cas9(D10a)-Nickase-TRE3G-Puro

pICH47742_2x35S-5'UTR-hCas9(STOP)-NOST

pAAVS1-Puro-DNR

pCLIP-Cas9(D10a)-Nickase-TRE3G-tRFP

pAC2-dual-dCas9VP48-sgExpression

pCLIP-Cas9(D10a)-Nickase-TRE3G-ZsGreen

pICSL01009_AtU6p

pwtCas9-bacteria

pCLIP-Cas9-Nuclease-EFS-Blast

pAC149-pCR8-dCas9VP160

pCLIP-Cas9-Nuclease-EFS-Puro

pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

pAC152-dual-dCas9VP64-sgExpression

pCLIP-Cas9-Nuclease-EFS-tRFP

pL-CRISPR.EFS.GFP

pAC153-dual-dCas9VP96-sgExpression

pCLIP-Cas9-Nuclease-EFS-ZsGreen

pL-CRISPR.EFS.tRFP

pAC154-dual-dCas9VP160-sgExpression

pCLIP-Cas9-Nuclease-hCMV-Blast

pL-CRISPR.SFFV.GFP

pAC155-pCR8-sgExpression

pCLIP-Cas9-Nuclease-hCMV-Puro

pL-CRISPR.SFFV.tRFP

pAC84-pCR8-dCas9

pCLIP-Cas9-Nuclease-hCMV-tRFP

pAC91-pmax-dCas9VP64

pCLIP-Cas9-Nuclease-hCMV-ZsGreen

pLenti-Cas9-IRES-Puro

pAC93-pmax-dCas9VP160

pCLIP-Cas9-Nuclease-TRE3G-Blast

pLenti-Cas9-P2A-tGFP

pAC94-pmax-dCas9VP160-2A-puro

pCLIP-Cas9-Nuclease-TRE3G-Puro

pLenti-Cas-Guide

pAC95-pmax-dCas9VP160-2A-neo

pCLIP-Cas9-Nuclease-TRE3G-tRFP

pLenti-EF1a-Cas9-IRES-Puro

pCLIP-Cas9-Nuclease-TRE3G-ZsGreen

pLenti-Guide-Puro

pCLIP-gRNA-EFS-Blast

pLKO.1-puro U6 sgRNA BfuAI stuffer

pCAG-T3-hCAS-pA

pCLIP-gRNA-EFS-Puro

pCAG-T3-hCasD10A-pA

pCLIP-gRNA-EFS-tRFP

pLV hUbC-Cas9-T2A-GFP

pCLIP-gRNA-EFS-ZsGreen

pLV hUbC-dCas9 VP64-T2A-GFP

pCas-Guide-AAVS1

pCLIP-gRNA-hCMV-Blast

pLV hUbC-dCas9-T2A-GFP

pCas-Guide-CRISPRa

pCLIP-gRNA-hCMV-Puro

pLVX-hyg-sgRNA1 Vector (Linear)

pY010 (pcDNA3.1-hAsCpf1)

pCas-Guide-CRISPRi

pCLIP-gRNA-hCMV-tRFP

pY016 (pcDNA3.1-hLbCpf1)

pCas-Guide-EF1a-CD4

pCLIP-gRNA-hCMV-ZsGreen

sgRNA(MS2) cloning backbone

pCas-Guide-EF1a-GFP

pCas-Guide-Nickase

pCas-Guide-ROSA26

Plasmid Sets

Basic Cloning Vectors

I.M.A.G.E. Consortium Plasmids

pET & Duet Vectors (Novagen)

TA and GC Cloning Vectors

Coronavirus Resources

Insect Cell Vectors

pGEX Vectors (GE Healthcare)

TOPO Cloning Vectors

CRISPR Plasmids

Luciferase Vectors

Viral Expression & Packaging Vectors

Fluorescent Protein Genes & Plasmids

Lucigen Vectors

Gateway® Cloning Vectors

Mammalian Expression Vectors

Structural Genomics Vectors

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