pAAV-Guide-it-1 Vector (Linear)

Linearized CRISPR/Cas9 adeno-associated virus (AAV) vector with S. aureus Cas9, for cloning and expression of a single guide RNA (sgRNA).

Sequence Author: Clontech (TaKaRa)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
Guide-it™ Sequencing Primer 1 (6950 .. 6973) SV40 NLS polIII terminator CMV enhancer ATG pAAV-Guide-it-1 Vector (Linear) 7028 bp
Guide-it™ Sequencing Primer 1
24-mer  /  38% GC
1 binding site
6950 .. 6973  =  24 annealed bases
Tm  =  56°C
ATG
3314 .. 3316  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
3314 .. 3316  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 NLS
3323 .. 3343  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
3323 .. 3343  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SaCas9
3344 .. 6499  =  3156 bp
1052 amino acids  =  123.8 kDa
Product: Cas9 (Csn1) endonuclease from the Staphylococcus aureus Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SaCas9
3344 .. 6499  =  3156 bp
1052 amino acids  =  123.8 kDa
Product: Cas9 (Csn1) endonuclease from the Staphylococcus aureus Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
6515 .. 6535  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
6515 .. 6535  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
FLAG
6536 .. 6559  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
6536 .. 6559  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
AmpR
603 .. 1463  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   603 .. 671  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
603 .. 1463  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   672 .. 1463  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
603 .. 1463  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1634 .. 2222  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1634 .. 2222  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
2776 .. 3079  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2776 .. 3079  =  304 bp
human cytomegalovirus immediate early enhancer
U6 promoter
6783 .. 7031  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
6783 .. 7031  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
bGH poly(A) signal
6569 .. 6776  =  208 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
6569 .. 6776  =  208 bp
bovine growth hormone polyadenylation signal
CMV promoter
3080 .. 3283  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
3080 .. 3283  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AAV2 ITR
96 .. 236  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
96 .. 236  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
2567 .. 2707  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
2567 .. 2707  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
498 .. 602  =  105 bp
AmpR promoter
498 .. 602  =  105 bp
Sa gRNA scaffold
1 .. 76  =  76 bp
guide RNA scaffold for the Staphylococcus aureus CRISPR/Cas9 system
Sa gRNA scaffold
1 .. 76  =  76 bp
guide RNA scaffold for the Staphylococcus aureus CRISPR/Cas9 system
polIII terminator
77 .. 81  =  5 bp
RNA polymerase III transcription terminator
polIII terminator
77 .. 81  =  5 bp
RNA polymerase III transcription terminator
Kozak sequence
3308 .. 3317  =  10 bp
vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)
Kozak sequence
3308 .. 3317  =  10 bp
vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)
ORF:  110 .. 391  =  282 bp
ORF:  93 amino acids  =  9.9 kDa
ORF:  3239 .. 6562  =  3324 bp
ORF:  1107 amino acids  =  130.0 kDa
ORF:  603 .. 1463  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3604 .. 3843  =  240 bp
ORF:  79 amino acids  =  8.5 kDa
ORF:  4705 .. 4962  =  258 bp
ORF:  85 amino acids  =  9.8 kDa
ORF:  6538 .. 6810  =  273 bp
ORF:  90 amino acids  =  10.0 kDa
ORF:  1067 .. 1333  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3287 .. 6478  =  3192 bp
ORF:  1063 amino acids  =  114.4 kDa
Click here to try SnapGene

Download pAAV-Guide-it-1 Vector (Linear).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.