pCRISPRa-Enhancer

Mammalian gene activation vector for expressing an MS2-p65-HSF1 fusion. Use together with pCas-Guide-CRISPRa.

Sequence Author: OriGene

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pCRISPRa-Enhancer 6300 bp
ATG
1029 .. 1031  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
1029 .. 1031  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
MS2-N55K
1032 .. 1418  =  387 bp
129 amino acids  =  13.7 kDa
Product: bacteriophage MS2 coat protein
binds with high affinity to a specific stem-loop structure in the viral RNA (Lim et al., 1994)
MS2-N55K
1032 .. 1418  =  387 bp
129 amino acids  =  13.7 kDa
Product: bacteriophage MS2 coat protein
binds with high affinity to a specific stem-loop structure in the viral RNA (Lim et al., 1994)
SV40 NLS
1473 .. 1493  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 (simian virus 40) large T antigen
SV40 NLS
1473 .. 1493  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 (simian virus 40) large T antigen
p65
1509 .. 2051  =  543 bp
181 amino acids  =  18.5 kDa
Product: C-terminal portion of the p65 subunit of mouse NF-κB
p65
1509 .. 2051  =  543 bp
181 amino acids  =  18.5 kDa
Product: C-terminal portion of the p65 subunit of mouse NF-κB
HSF1
2076 .. 2447  =  372 bp
124 amino acids  =  13.0 kDa
Product: C-terminal activation domain from the human heat shock transcription factor HSF1
HSF1
2076 .. 2447  =  372 bp
124 amino acids  =  13.0 kDa
Product: C-terminal activation domain from the human heat shock transcription factor HSF1
Myc
2466 .. 2495  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
Myc
2466 .. 2495  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
FLAG
2514 .. 2537  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
2514 .. 2537  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
NeoR/KanR
4551 .. 5345  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
4551 .. 5345  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
hGH poly(A) signal
2584 .. 3206  =  623 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
2584 .. 3206  =  623 bp
human growth hormone polyadenylation signal
ori
3355 .. 3943  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3355 .. 3943  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
5870 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5870 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
343 .. 722  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
343 .. 722  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
5380 .. 5737  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
5380 .. 5737  =  358 bp
SV40 enhancer and early promoter
CMV promoter
723 .. 926  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
723 .. 926  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
5739 .. 5843  =  105 bp
AmpR promoter
5739 .. 5843  =  105 bp
HSV TK poly(A) signal
4272 .. 4319  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
4272 .. 4319  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
SV40 ori
5394 .. 5529  =  136 bp
SV40 origin of replication
SV40 ori
5394 .. 5529  =  136 bp
SV40 origin of replication
ORF:  882 .. 2543  =  1662 bp
ORF:  553 amino acids  =  57.5 kDa
ORF:  4092 .. 4325  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  4500 .. 5036  =  537 bp
ORF:  178 amino acids  =  19.9 kDa
ORF:  4081 .. 4530  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  4551 .. 5345  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  1856 .. 2140  =  285 bp
ORF:  94 amino acids  =  9.6 kDa
ORF:  2948 .. 3172  =  225 bp
ORF:  74 amino acids  =  8.1 kDa
ORF:  4787 .. 5173  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
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Download pCRISPRa-Enhancer.dna file

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