pHAGE EF1-alpha dCas9-VP64

Lentiviral CRISPR activation vector for constitutive expression of catalytically inactive dCas9 fused to the VP64 transcriptional activation domain.

Sequence Author: Maehr

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HomePlasmidsCRISPR PlasmidspHAGE EF1-alpha dCas9-VP64
attB2 pHAGE EF1 α dCas9-VP64 12,794 bp
dCas9
8340 .. 12,443  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 1:  
   8340 .. 8366  =  27 bp
   9 amino acids  =  1.1 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
8340 .. 12,443  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 2:  
   8367 .. 8369  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
8340 .. 12,443  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 3:  
   8370 .. 10,856  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
8340 .. 12,443  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 4:  
   10,857 .. 10,859  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
8340 .. 12,443  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 5:  
   10,860 .. 12,443  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
8340 .. 12,443  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
SV40 NLS
12,456 .. 12,476  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
12,456 .. 12,476  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
3xHA
12,477 .. 12,566  =  90 bp
30 amino acids  =  3.5 kDa
Product: three tandem HA epitope tags
3xHA
12,477 .. 12,566  =  90 bp
30 amino acids  =  3.5 kDa
Product: three tandem HA epitope tags
VP64
12,588 .. 12,737  =  150 bp
50 amino acids  =  5.5 kDa
Product: tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)
VP64
12,588 .. 12,737  =  150 bp
50 amino acids  =  5.5 kDa
Product: tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)
EF-1α promoter
7093 .. 8271  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
EF-1α promoter
7093 .. 8271  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
AmpR
2468 .. 3328  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   2468 .. 2536  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2468 .. 3328  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   2537 .. 3328  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2468 .. 3328  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
LTR
4873 .. 5506  =  634 bp
long terminal repeat (LTR) from HIV-1
LTR
4873 .. 5506  =  634 bp
long terminal repeat (LTR) from HIV-1
PuroR
646 .. 1245  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
646 .. 1245  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
WPRE
1262 .. 1850  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
1262 .. 1850  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
3499 .. 4087  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3499 .. 4087  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
PGK promoter
126 .. 625  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
126 .. 625  =  500 bp
mouse phosphoglycerate kinase 1 promoter
LTR (ΔU3)
1925 .. 2158  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
LTR (ΔU3)
1925 .. 2158  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
RRE
6175 .. 6408  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
6175 .. 6408  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
HIV-1 Ψ
5553 .. 5678  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
5553 .. 5678  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
6930 .. 7047  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
6930 .. 7047  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
2363 .. 2467  =  105 bp
AmpR promoter
2363 .. 2467  =  105 bp
attB1
8306 .. 8330  =  25 bp
recombination site for the Gateway® BP reaction
attB1
8306 .. 8330  =  25 bp
recombination site for the Gateway® BP reaction
attB2
12,784 .. 14  =  25 bp
recombination site for the Gateway® BP reaction
attB2
12,784 .. 14  =  25 bp
recombination site for the Gateway® BP reaction
EF-1α intron A
7324 .. 8262  =  939 bp
intron upstream of the start codon of human EF-1α
EF-1α intron A
7324 .. 8262  =  939 bp
intron upstream of the start codon of human EF-1α
ORF:  406 .. 1245  =  840 bp
ORF:  279 amino acids  =  30.1 kDa
ORF:  6910 .. 7170  =  261 bp
ORF:  86 amino acids  =  9.7 kDa
ORF:  12,406 .. 12,738  =  333 bp
ORF:  110 amino acids  =  12.5 kDa
ORF:  1352 .. 1864  =  513 bp
ORF:  170 amino acids  =  18.6 kDa
ORF:  2468 .. 3328  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  6053 .. 6979  =  927 bp
ORF:  308 amino acids  =  35.4 kDa
ORF:  7649 .. 8086  =  438 bp
ORF:  145 amino acids  =  15.9 kDa
ORF:  1365 .. 1898  =  534 bp
ORF:  177 amino acids  =  18.8 kDa
ORF:  7728 .. 7961  =  234 bp
ORF:  77 amino acids  =  7.7 kDa
ORF:  8340 .. 12,746  =  4407 bp
ORF:  1468 amino acids  =  169.2 kDa
ORF:  6753 .. 7001  =  249 bp
ORF:  82 amino acids  =  9.0 kDa
ORF:  8259 .. 8750  =  492 bp
ORF:  163 amino acids  =  17.9 kDa
ORF:  9744 .. 10,262  =  519 bp
ORF:  172 amino acids  =  20.1 kDa
ORF:  10,326 .. 10,601  =  276 bp
ORF:  91 amino acids  =  10.3 kDa
ORF:  11,229 .. 12,386  =  1158 bp
ORF:  385 amino acids  =  43.7 kDa
ORF:  7391 .. 8011  =  621 bp
ORF:  206 amino acids  =  23.0 kDa
ORF:  2932 .. 3198  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5887 .. 6348  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
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pcDNA-dCas9-p300 Core (D1399Y)
pdCas9
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pcDNA-dCas9-VP64
pdCas9-bacteria
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pCFD2-dU6-2gRNA
pDCC6
pRGE31
gRNA_AAVS1-T1
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pDD162 (Peft-3_Cas9 + Empty sgRNA)
pRGEB31
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pDR274
pRGEB32
gRNA_Cloning Vector
pCLIP-All-EFS-Ametrine
Peft-3_cas9-SV40_NLS_tbb-2 3'UTR
pROSA26-Puro-DNR
gRNA_GFP-T1
pCLIP-All-EFS-Blast
pET-28b-Cas9-His
pRPR1_gRNA_handle_RPR1t
gRNA_GFP-T2
pCLIP-All-EFS-Puro
pET-Cas9-6xHis
pSECC
HBT-pcoCas9
pCLIP-All-EFS-tRFP
pET-Cas9-NLS-6xHis
pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
hCas9
pCLIP-All-EFS-ZsGreen
pET-NLS-Cas9-6xHis
pSLQ1658-dCas9-EGFP
hCas9_D10A
pCLIP-All-hCMV-Blast
pET28a Cas9-Cys
pSpCas9(BB)-2A-GFP (PX458)
JDS246
pCLIP-All-hCMV-Puro
pFGC-pcoCas9
pSpCas9(BB)-2A-Puro (PX459) V2.0
lenti dCAS-VP64_Blast
pCLIP-All-hCMV-tRFP
pGL3-U6-sgRNA-PGK-puromycin
pSpCas9n(BB)-2A-GFP (PX461)
lenti MS2-P65-HSF1_Hygro
pCLIP-All-hCMV-ZsGreen
pgRNA-bacteria
pSpCas9n(BB)-2A-Puro (PX462) V2.0
lenti sgRNA(MS2)_zeo backbone
pCLIP-All-Nickase(D10A)-EFS-Blast
pgRNA-humanized
pSpCas9n(BB) (PX460)
lentiCas9-Blast
pCLIP-All-Nickase(D10A)-EFS-Puro
pGuide
pSpCas9 (PX165)
lentiCas9-EGFP
pCLIP-All-Nickase(D10A)-EFS-tRFP
pGuide-it-sgRNA1 Vector (Linear)
pSPgRNA
lentiCas9n(D10A)-Blast
pCLIP-All-Nickase(D10A)-EFS-ZsGreen
pGuide-it-tdTomato Vector (Linear)
pSQT834
lentiCRISPR v2
pCLIP-All-Nickase(D10A)-hCMV-Puro
pGuide-it-ZsGreen1 Vector (Linear)
pSQT1313
lentiGuide-Puro
pCLIP-All-Nickase(D10A)-hCMV-tRFP
pHAGE EF1-alpha dCas9-KRAB
pST1374-N-NLS-flag-linker-Cas9-D10A
LSL-Cas9-Rosa26TV
pCLIP-All-Nickase(D10A)-hCMV-ZsGreen
pHAGE EF1-alpha dCas9-VP64
pST1374-N-NLS-flag-linker-Cas9-H840A
M-SPcas
pCLIP-All-Nickase(D10A)-TRE3G-Blast
pHAGE TRE dCas9
pST1374-NLS-flag-linker-Cas9
M-ST1cas
pCLIP-All-Nickase(D10A)-TRE3G-Puro
pHAGE TRE dCas9-KRAB
pT3TS-nCas9n
MLM3613
pCLIP-All-Nickase(D10A)-TRE3G-tRFP
pHAGE TRE dCas9-VP64
pT7-Cas9
MLM3636
pCLIP-All-Nickase(D10A)-TRE3G-ZsGreen
pHAGE-TO-dCas9-3XGFP
pT7-Cas9-Nickase
MLM3639
pCLIP-All-TRE3G-Blast
pHAGE-TO-dCas9-3XmCherry
pT7-gRNA
MS2-P65-HSF1_GFP
pCLIP-All-TRE3G-Puro
pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2
pT7-Guide-IVT
MSP1101
pCLIP-All-TRE3G-tRFP
pHAGE-TO-nmdCas9-3XGFP
pTDH3-dCas9
MSP2133
pCLIP-All-TRE3G-ZsGreen
pHAGE-TO-nmdCas9-3XmCherry
pTDH3-dCas9-Mxi1
MSP2135
pCLIP-Cas9(D10a)-Nickase-EFS-Puro
pHR-SFFV-dCas9-BFP
pTOL-hCMV-TET3G-Hygro Transactivator
MSP469
pCLIP-Cas9(D10a)-Nickase-EFS-Blast
pHR-SFFV-dCas9-BFP-KRAB
pTPGI_dCas9_VP64
p3s-Cas9HC
pCLIP-Cas9(D10a)-Nickase-EFS-tRFP
pHR-SFFV-KRAB-dCas9-P2A-mCherry
pU6-BbsI-chiRNA
p414-TEF1p-Cas9-CYC1t
pCLIP-Cas9(D10a)-Nickase-EFS-ZsGreen
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
PU6_klp-12_sgRNA
p415-GalL-Cas9-CYC1t
pCLIP-Cas9(D10a)-Nickase-hCMV-Blast
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
p426-SNR52p-gRNA.CAN1.Y-SUP4t
pCLIP-Cas9(D10a)-Nickase-hCMV-Puro
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6
pAAV-Guide-it-1 Vector (Linear)
pCLIP-Cas9(D10a)-Nickase-hCMV-tRFP
pHSN401
pUC119-gRNA
pAAV-Guide-it-Down Vector (Linear)
pCLIP-Cas9(D10a)-Nickase-hCMV-ZsGreen
pHsp70-Cas9
pUC57-sgRNA expression vector
pAAV-Guide-it-Up Vector
pCLIP-Cas9(D10a)-Nickase-TRE3G-Blast
pICH41308_hCas9
Puro-Cas9 donor
pAAVS1-BSD-DNR
pCLIP-Cas9(D10a)-Nickase-TRE3G-Puro
pICH47742_2x35S-5'UTR-hCas9(STOP)-NOST
pWJ66
pAAVS1-Puro-DNR
pCLIP-Cas9(D10a)-Nickase-TRE3G-tRFP
piCRg Entry
pWJ68
pAC2-dual-dCas9VP48-sgExpression
pCLIP-Cas9(D10a)-Nickase-TRE3G-ZsGreen
pICSL01009_AtU6p
pwtCas9-bacteria
pAc-sgRNA-Cas9
pCLIP-Cas9-Nuclease-EFS-Blast
pK7WGF2_hCas9
pX260
pAC149-pCR8-dCas9VP160
pCLIP-Cas9-Nuclease-EFS-Puro
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
pX330
pAC152-dual-dCas9VP64-sgExpression
pCLIP-Cas9-Nuclease-EFS-tRFP
pL-CRISPR.EFS.GFP
pX330A-1x2
pAC153-dual-dCas9VP96-sgExpression
pCLIP-Cas9-Nuclease-EFS-ZsGreen
pL-CRISPR.EFS.tRFP
pX330S-2
pAC154-dual-dCas9VP160-sgExpression
pCLIP-Cas9-Nuclease-hCMV-Blast
pL-CRISPR.SFFV.GFP
pX334
pAC155-pCR8-sgExpression
pCLIP-Cas9-Nuclease-hCMV-Puro
pL-CRISPR.SFFV.tRFP
pX335
pAC84-pCR8-dCas9
pCLIP-Cas9-Nuclease-hCMV-tRFP
pLenti-Cas9
PX551
pAC91-pmax-dCas9VP64
pCLIP-Cas9-Nuclease-hCMV-ZsGreen
pLenti-Cas9-IRES-Puro
PX552
pAC93-pmax-dCas9VP160
pCLIP-Cas9-Nuclease-TRE3G-Blast
pLenti-Cas9-P2A-tGFP
pX600
pAC94-pmax-dCas9VP160-2A-puro
pCLIP-Cas9-Nuclease-TRE3G-Puro
pLenti-Cas-Guide
pX601
pAC95-pmax-dCas9VP160-2A-neo
pCLIP-Cas9-Nuclease-TRE3G-tRFP
pLenti-EF1a-Cas9-IRES-Puro
pX602
pACT2-CAS9c
pCLIP-Cas9-Nuclease-TRE3G-ZsGreen
pLenti-Guide-Puro
PX851
pBS-Hsp70-Cas9
pCLIP-gRNA-EFS-Blast
pLKO.1-puro U6 sgRNA BfuAI stuffer
PX852
pCAG-T3-hCAS-pA
pCLIP-gRNA-EFS-Puro
pLSC-5
PX853
pCAG-T3-hCasD10A-pA
pCLIP-gRNA-EFS-tRFP
pLV hUbC-Cas9-T2A-GFP
PX854
pCas
pCLIP-gRNA-EFS-ZsGreen
pLV hUbC-dCas9 VP64-T2A-GFP
PX855
pCas-Guide-AAVS1
pCLIP-gRNA-hCMV-Blast
pLV hUbC-dCas9-T2A-GFP
PX856
pCas-Guide-CRISPRa
pCLIP-gRNA-hCMV-Puro
pLVX-hyg-sgRNA1 Vector (Linear)
pY010 (pcDNA3.1-hAsCpf1)
pCas-Guide-CRISPRi
pCLIP-gRNA-hCMV-tRFP
pLVX-puro-Cas9
pY016 (pcDNA3.1-hLbCpf1)
pCas-Guide-EF1a-CD4
pCLIP-gRNA-hCMV-ZsGreen
pLX-sgRNA
sgRNA(MS2) cloning backbone
pCas-Guide-EF1a-GFP
pCRCT
pMB66
SP-Cas9
pCas-Guide-Nickase
pCRISPomyces-1
pMB70
SP-dCas9-VPR
pCas-Guide-ROSA26
pCRISPomyces-2
pME-Cas9
VP12
pCas9
pCRISPR

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