3xFLAG-dCas9 pCMV-7.1

Plasmid for expression in mammalian cells of FLAG®-tagged catalytically inactive dCas9, for engineered ChIP (enChIP) purification of specific genomic regions.

Sequence Author: Fujii Lab / Addgene #47948

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CMV enhancer ATG 3xFLAG-dCas9/pCMV-7.1 8848 bp
ATG
928 .. 930  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
928 .. 930  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
3xFLAG
931 .. 996  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
3xFLAG
931 .. 996  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
dCas9
1000 .. 5103  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 1:  
   1000 .. 1026  =  27 bp
   9 amino acids  =  1.1 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1000 .. 5103  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 2:  
   1027 .. 1029  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1000 .. 5103  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 3:  
   1030 .. 3516  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1000 .. 5103  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 4:  
   3517 .. 3519  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1000 .. 5103  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 5:  
   3520 .. 5103  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1000 .. 5103  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
SV40 NLS
5116 .. 5136  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5116 .. 5136  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
AmpR
7401 .. 8261  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   7401 .. 8192  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7401 .. 8261  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   8193 .. 8261  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7401 .. 8261  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
hGH poly(A) signal
5189 .. 5811  =  623 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
5189 .. 5811  =  623 bp
human growth hormone polyadenylation signal
ori
6642 .. 7230  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6642 .. 7230  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
8393 .. 8848  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
8393 .. 8848  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
318 .. 697  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
318 .. 697  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
5840 .. 6169  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
5840 .. 6169  =  330 bp
SV40 enhancer and early promoter
CMV promoter
698 .. 901  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
698 .. 901  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
8262 .. 8366  =  105 bp
AmpR promoter
8262 .. 8366  =  105 bp
SV40 ori
6020 .. 6155  =  136 bp
SV40 origin of replication
SV40 ori
6020 .. 6155  =  136 bp
SV40 origin of replication
ORF:  928 .. 5139  =  4212 bp
ORF:  1403 amino acids  =  162.6 kDa
ORF:  7531 .. 7797  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5066 .. 5302  =  237 bp
ORF:  78 amino acids  =  8.6 kDa
ORF:  922 .. 1479  =  558 bp
ORF:  185 amino acids  =  20.7 kDa
ORF:  2404 .. 2922  =  519 bp
ORF:  172 amino acids  =  20.1 kDa
ORF:  2986 .. 3261  =  276 bp
ORF:  91 amino acids  =  10.3 kDa
ORF:  4513 .. 5046  =  534 bp
ORF:  177 amino acids  =  20.0 kDa
ORF:  5050 .. 5289  =  240 bp
ORF:  79 amino acids  =  8.7 kDa
ORF:  5553 .. 5777  =  225 bp
ORF:  74 amino acids  =  8.1 kDa
ORF:  7401 .. 8261  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Download 3xFLAG-dCas9 pCMV-7.1.dna file

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