pY016 (pcDNA3.1-hLbCpf1)

Zhang lab mammalian expression vector encoding a humanized version of the Lachnospiraceae bacterium CRISPR-associated protein Cpf1.

Sequence Author: Zhang Lab / Addgene #69988

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nucleoplasmin NLS CMV enhancer pY016 (pcDNA3.1-hLbCpf1) 9227 bp
LbCpf1
929 .. 4612  =  3684 bp
1228 amino acids  =  143.7 kDa
Product: CRISPR-associated protein from Lachnospiraceae bacterium ND2006 (Zetsche et al., 2015)
LbCpf1
929 .. 4612  =  3684 bp
1228 amino acids  =  143.7 kDa
Product: CRISPR-associated protein from Lachnospiraceae bacterium ND2006 (Zetsche et al., 2015)
nucleoplasmin NLS
4613 .. 4660  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
nucleoplasmin NLS
4613 .. 4660  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
3xHA
4667 .. 4747  =  81 bp
27 amino acids  =  3.3 kDa
Product: three tandem HA epitope tags
3xHA
4667 .. 4747  =  81 bp
27 amino acids  =  3.3 kDa
Product: three tandem HA epitope tags
AmpR
8231 .. 9091  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   8231 .. 9022  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8231 .. 9091  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   9023 .. 9091  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8231 .. 9091  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
5935 .. 6729  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
5935 .. 6729  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
7475 .. 8060  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7475 .. 8060  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
5097 .. 5525  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5097 .. 5525  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
5539 .. 5868  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
5539 .. 5868  =  330 bp
SV40 enhancer and early promoter
bGH poly(A) signal
4827 .. 5051  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
4827 .. 5051  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
6903 .. 7024  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
6903 .. 7024  =  122 bp
SV40 polyadenylation signal
AmpR promoter
9092 .. 9196  =  105 bp
AmpR promoter
9092 .. 9196  =  105 bp
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
5719 .. 5854  =  136 bp
SV40 origin of replication
SV40 ori
5719 .. 5854  =  136 bp
SV40 origin of replication
ORF:  5935 .. 6729  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  929 .. 4750  =  3822 bp
ORF:  1273 amino acids  =  148.8 kDa
ORF:  5048 .. 5314  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  6107 .. 6493  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  8361 .. 8627  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  8231 .. 9091  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1807 .. 2184  =  378 bp
ORF:  125 amino acids  =  14.3 kDa
ORF:  6244 .. 6780  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
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