pICH41308_hCas9

Level 0 hCas9 module plasmid encoding human codon-optimized Cas9 with a nuclear localization signal.

Sequence Author: Kamoun Lab / Addgene #49770

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pICH41308::hCas9 6388 bp
Cas9
2233 .. 6336  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
2233 .. 6336  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
6349 .. 6369  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
6349 .. 6369  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SmR
434 .. 1225  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
SmR
434 .. 1225  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
ori
1396 .. 1984  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1396 .. 1984  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
AmpR promoter
110 .. 214  =  105 bp
AmpR promoter
110 .. 214  =  105 bp
ORF:  2233 .. 6372  =  4140 bp
ORF:  1379 amino acids  =  159.7 kDa
ORF:  215 .. 1225  =  1011 bp
ORF:  336 amino acids  =  37.5 kDa
ORF:  473 .. 856  =  384 bp
ORF:  127 amino acids  =  14.6 kDa
ORF:  947 .. 1186  =  240 bp
ORF:  79 amino acids  =  8.8 kDa
ORF:  466 .. 723  =  258 bp
ORF:  85 amino acids  =  9.1 kDa
ORF:  733 .. 1011  =  279 bp
ORF:  92 amino acids  =  9.4 kDa
ORF:  2212 .. 2712  =  501 bp
ORF:  166 amino acids  =  18.6 kDa
ORF:  3637 .. 4155  =  519 bp
ORF:  172 amino acids  =  20.1 kDa
ORF:  4219 .. 4752  =  534 bp
ORF:  177 amino acids  =  19.9 kDa
ORF:  5746 .. 6279  =  534 bp
ORF:  177 amino acids  =  20.0 kDa
ORF:  4152 .. 4403  =  252 bp
ORF:  83 amino acids  =  9.9 kDa
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Download pICH41308_hCas9.dna file

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