pT7-Guide-IVT

In vitro transcription vector with the T7 promoter, for producing a guide RNA (gRNA).

Sequence Author: OriGene

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No matches
BsmBI (3300) BsmBI (3241) EcoRI (3222) EcoRI (3389) pT7-Guide-IVT 3444 bp
BsmBI  (3300)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
BsmBI  (3241)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
EcoRI  (3222)
2 sites
G A A T T C C T T A A G
EcoRI  (3389)
2 sites
G A A T T C C T T A A G
AmpR
1106 .. 1966  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1106 .. 1174  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1106 .. 1966  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1175 .. 1966  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1106 .. 1966  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2137 .. 2725  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2137 .. 2725  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
264 .. 719  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
264 .. 719  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
AmpR promoter
1001 .. 1105  =  105 bp
AmpR promoter
1001 .. 1105  =  105 bp
gRNA scaffold
3300 .. 3375  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
3300 .. 3375  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
T7 promoter
3228 .. 3246  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
3228 .. 3246  =  19 bp
promoter for bacteriophage T7 RNA polymerase
M13 fwd
35 .. 51  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
35 .. 51  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  1106 .. 1966  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3402 .. 287  =  330 bp
ORF:  109 amino acids
ORF:  1570 .. 1836  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  3323 .. 244  =  366 bp
ORF:  121 amino acids  =  14.1 kDa
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Download pT7-Guide-IVT.dna file

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