pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

CRISPR activation vector encoding TagBFP plus catalytically inactive dCas9 fused to 10 copies of an optimized GCN4_v4 peptide.

Sequence Author: Vale Lab / Addgene #60903

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HomePlasmidsCRISPR PlasmidspHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
SV40 NLS NotI (12,838) BamHI (12,043) SV40 NLS SV40 NLS SV40 NLS ATG pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP 14,551 bp
NotI  (12,838)
1 site		 G C G G C C G C C G C C G G C G
BamHI  (12,043)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
ATG
7732 .. 7734  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
7732 .. 7734  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 NLS
7735 .. 7755  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
7735 .. 7755  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 1:  
   7810 .. 7836  =  27 bp
   9 amino acids  =  1.1 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 2:  
   7837 .. 7839  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 3:  
   7840 .. 10,326  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 4:  
   10,327 .. 10,329  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 5:  
   10,330 .. 11,913  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
HA
11,917 .. 11,943  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
11,917 .. 11,943  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
SV40 NLS
11,962 .. 11,982  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
11,962 .. 11,982  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
11,989 .. 12,009  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
11,989 .. 12,009  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 1:  GCN4_v4  
   12,073 .. 12,129  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 2:  
   12,130 .. 12,144  =  15 bp
   5 amino acids  =  363.3 Da
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 3:  GCN4_v4  
   12,145 .. 12,201  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 4:  
   12,202 .. 12,216  =  15 bp
   5 amino acids  =  363.3 Da
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 5:  GCN4_v4  
   12,217 .. 12,273  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 6:  
   12,274 .. 12,288  =  15 bp
   5 amino acids  =  363.3 Da
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 7:  GCN4_v4  
   12,289 .. 12,345  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 8:  
   12,346 .. 12,360  =  15 bp
   5 amino acids  =  363.3 Da
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 9:  GCN4_v4  
   12,361 .. 12,417  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 10:  
   12,418 .. 12,432  =  15 bp
   5 amino acids  =  363.3 Da
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 11:  GCN4_v4  
   12,433 .. 12,489  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 12:  
   12,490 .. 12,504  =  15 bp
   5 amino acids  =  363.3 Da
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 13:  GCN4_v4  
   12,505 .. 12,561  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 14:  
   12,562 .. 12,576  =  15 bp
   5 amino acids  =  363.3 Da
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 15:  GCN4_v4  
   12,577 .. 12,633  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 16:  
   12,634 .. 12,648  =  15 bp
   5 amino acids  =  363.3 Da
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 17:  GCN4_v4  
   12,649 .. 12,705  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 18:  
   12,706 .. 12,720  =  15 bp
   5 amino acids  =  363.3 Da
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
   Segment 19:  GCN4_v4  
   12,721 .. 12,777  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
10xGCN4_v4
12,073 .. 12,777  =  705 bp
235 amino acids  =  26.1 kDa
19 segments
Product: 10 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
SV40 NLS
12,859 .. 12,879  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
12,859 .. 12,879  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
P2A
12,889 .. 12,945  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1 polyprotein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
P2A
12,889 .. 12,945  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1 polyprotein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
TagBFP
12,958 .. 13,656  =  699 bp
233 amino acids  =  26.3 kDa
Product: monomeric blue fluorescent protein
mammalian codon-optimized
TagBFP
12,958 .. 13,656  =  699 bp
233 amino acids  =  26.3 kDa
Product: monomeric blue fluorescent protein
mammalian codon-optimized
SV40 NLS
13,666 .. 13,686  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
13,666 .. 13,686  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
AmpR
1014 .. 1874  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1014 .. 1082  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1014 .. 1874  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1083 .. 1874  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1014 .. 1874  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
LTR
5285 .. 5918  =  634 bp
long terminal repeat (LTR) from HIV-1
LTR
5285 .. 5918  =  634 bp
long terminal repeat (LTR) from HIV-1
ori
2045 .. 2633  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2045 .. 2633  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
WPRE
13,708 .. 14,296  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
13,708 .. 14,296  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
RRE
6582 .. 6815  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
6582 .. 6815  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
LTR (ΔU3)
14,395 .. 77  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
LTR (ΔU3)
14,395 .. 77  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
SV40 promoter
7509 .. 7719  =  211 bp
SV40 early promoter
SV40 promoter
7509 .. 7719  =  211 bp
SV40 early promoter
SV40 poly(A) signal
4982 .. 5116  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4982 .. 5116  =  135 bp
SV40 polyadenylation signal
HIV-1 Ψ
5965 .. 6090  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
5965 .. 6090  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
7329 .. 7446  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
7329 .. 7446  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
909 .. 1013  =  105 bp
AmpR promoter
909 .. 1013  =  105 bp
SV40 ori
7578 .. 7713  =  136 bp
SV40 origin of replication
SV40 ori
7578 .. 7713  =  136 bp
SV40 origin of replication
ORF:  310 .. 672  =  363 bp
ORF:  120 amino acids  =  12.4 kDa
ORF:  4486 .. 4959  =  474 bp
ORF:  157 amino acids  =  18.2 kDa
ORF:  6460 .. 7557  =  1098 bp
ORF:  365 amino acids  =  42.1 kDa
ORF:  7732 .. 13,689  =  5958 bp
ORF:  1985 amino acids  =  225.8 kDa
ORF:  13,798 .. 14,334  =  537 bp
ORF:  178 amino acids  =  19.6 kDa
ORF:  3491 .. 3865  =  375 bp
ORF:  124 amino acids  =  14.0 kDa
ORF:  13,811 .. 14,323  =  513 bp
ORF:  170 amino acids  =  17.9 kDa
ORF:  1014 .. 1874  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3645 .. 3926  =  282 bp
ORF:  93 amino acids  =  10.7 kDa
ORF:  3957 .. 4340  =  384 bp
ORF:  127 amino acids  =  14.9 kDa
ORF:  1478 .. 1744  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3539 .. 3958  =  420 bp
ORF:  139 amino acids  =  15.9 kDa
ORF:  226 .. 537  =  312 bp
ORF:  103 amino acids  =  10.7 kDa
ORF:  7774 .. 12,099  =  4326 bp
ORF:  1441 amino acids  =  152.0 kDa
ORF:  12,832 .. 13,464  =  633 bp
ORF:  210 amino acids  =  21.8 kDa
ORF:  6441 .. 6755  =  315 bp
ORF:  104 amino acids  =  11.0 kDa
ORF:  7713 .. 7937  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  9159 .. 9452  =  294 bp
ORF:  97 amino acids  =  10.7 kDa
ORF:  9723 .. 10,172  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
ORF:  12,744 .. 13,001  =  258 bp
ORF:  85 amino acids  =  9.1 kDa
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Individual Sequences & Maps

3xFLAG-dCas9 pCMV-7.1
pCas9_GFP
pCRISPRa-Enhancer
pME-Cas9-T2A-GFP
3xFLAG-dCas9 pMXs-neo
pCas9D10A_GFP
pCS2+hSpCas9
pMJ806
3xFLAG-dCas9 pMXs-puro
pCas-Guide
pCS2-Cas9
pMJ841
Cas9m4
pcDNA-dCas9
pCS2-nCas9n
pMJ920
Cas9m4-VP64
pcDNA-dCas9-p300 Core
pCW-Cas9
pMLM3705
CRISPRi library backbone
pcDNA-dCas9-p300 Core (D1399Y)
pdCas9
pMSCV-LTR-dCas9-p65AD-BFP
dCAS9-VP64_GFP
pcDNA-dCas9-VP64
pdCas9-bacteria
pMSCV-LTR-dCas9-VP64-BFP
eSpCas9(1.1)
pcDNA3.1-CibN-dCas9-CibN
pdCas9-humanized
pMZ374
GeneArt CRISPR Nuclease Vector - CD4 (linearized)
pCFD1-dU6-1gRNA
pdCas9 BFP-humanized
pNW3
GeneArt CRISPR Nuclease Vector - OFP (linearized)
pCFD2-dU6-2gRNA
pDCC6
pRGE31
gRNA_AAVS1-T1
pCFD3-dU6-3gRNA
pDD162 (Peft-3_Cas9 + Empty sgRNA)
pRGEB31
gRNA_AAVS1-T2
pCFD4-U6-1_U6-3tandemgRNAs
pDR274
pRGEB32
gRNA_Cloning Vector
pCLIP-All-EFS-Ametrine
Peft-3_cas9-SV40_NLS_tbb-2 3'UTR
pROSA26-Puro-DNR
gRNA_GFP-T1
pCLIP-All-EFS-Blast
pET-28b-Cas9-His
pRPR1_gRNA_handle_RPR1t
gRNA_GFP-T2
pCLIP-All-EFS-Puro
pET-Cas9-6xHis
pSECC
HBT-pcoCas9
pCLIP-All-EFS-tRFP
pET-Cas9-NLS-6xHis
pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
hCas9
pCLIP-All-EFS-ZsGreen
pET-NLS-Cas9-6xHis
pSLQ1658-dCas9-EGFP
hCas9_D10A
pCLIP-All-hCMV-Blast
pET28a Cas9-Cys
pSpCas9(BB)-2A-GFP (PX458)
JDS246
pCLIP-All-hCMV-Puro
pFGC-pcoCas9
pSpCas9(BB)-2A-Puro (PX459) V2.0
lenti dCAS-VP64_Blast
pCLIP-All-hCMV-tRFP
pGL3-U6-sgRNA-PGK-puromycin
pSpCas9n(BB)-2A-GFP (PX461)
lenti MS2-P65-HSF1_Hygro
pCLIP-All-hCMV-ZsGreen
pgRNA-bacteria
pSpCas9n(BB)-2A-Puro (PX462) V2.0
lenti sgRNA(MS2)_zeo backbone
pCLIP-All-Nickase(D10A)-EFS-Blast
pgRNA-humanized
pSpCas9n(BB) (PX460)
lentiCas9-Blast
pCLIP-All-Nickase(D10A)-EFS-Puro
pGuide
pSpCas9 (PX165)
lentiCas9-EGFP
pCLIP-All-Nickase(D10A)-EFS-tRFP
pGuide-it-sgRNA1 Vector (Linear)
pSPgRNA
lentiCas9n(D10A)-Blast
pCLIP-All-Nickase(D10A)-EFS-ZsGreen
pGuide-it-tdTomato Vector (Linear)
pSQT834
lentiCRISPR v2
pCLIP-All-Nickase(D10A)-hCMV-Puro
pGuide-it-ZsGreen1 Vector (Linear)
pSQT1313
lentiGuide-Puro
pCLIP-All-Nickase(D10A)-hCMV-tRFP
pHAGE EF1-alpha dCas9-KRAB
pST1374-N-NLS-flag-linker-Cas9-D10A
LSL-Cas9-Rosa26TV
pCLIP-All-Nickase(D10A)-hCMV-ZsGreen
pHAGE EF1-alpha dCas9-VP64
pST1374-N-NLS-flag-linker-Cas9-H840A
M-SPcas
pCLIP-All-Nickase(D10A)-TRE3G-Blast
pHAGE TRE dCas9
pST1374-NLS-flag-linker-Cas9
M-ST1cas
pCLIP-All-Nickase(D10A)-TRE3G-Puro
pHAGE TRE dCas9-KRAB
pT3TS-nCas9n
MLM3613
pCLIP-All-Nickase(D10A)-TRE3G-tRFP
pHAGE TRE dCas9-VP64
pT7-Cas9
MLM3636
pCLIP-All-Nickase(D10A)-TRE3G-ZsGreen
pHAGE-TO-dCas9-3XGFP
pT7-Cas9-Nickase
MLM3639
pCLIP-All-TRE3G-Blast
pHAGE-TO-dCas9-3XmCherry
pT7-gRNA
MS2-P65-HSF1_GFP
pCLIP-All-TRE3G-Puro
pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2
pT7-Guide-IVT
MSP1101
pCLIP-All-TRE3G-tRFP
pHAGE-TO-nmdCas9-3XGFP
pTDH3-dCas9
MSP2133
pCLIP-All-TRE3G-ZsGreen
pHAGE-TO-nmdCas9-3XmCherry
pTDH3-dCas9-Mxi1
MSP2135
pCLIP-Cas9(D10a)-Nickase-EFS-Puro
pHR-SFFV-dCas9-BFP
pTOL-hCMV-TET3G-Hygro Transactivator
MSP469
pCLIP-Cas9(D10a)-Nickase-EFS-Blast
pHR-SFFV-dCas9-BFP-KRAB
pTPGI_dCas9_VP64
p3s-Cas9HC
pCLIP-Cas9(D10a)-Nickase-EFS-tRFP
pHR-SFFV-KRAB-dCas9-P2A-mCherry
pU6-BbsI-chiRNA
p414-TEF1p-Cas9-CYC1t
pCLIP-Cas9(D10a)-Nickase-EFS-ZsGreen
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
PU6_klp-12_sgRNA
p415-GalL-Cas9-CYC1t
pCLIP-Cas9(D10a)-Nickase-hCMV-Blast
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
p426-SNR52p-gRNA.CAN1.Y-SUP4t
pCLIP-Cas9(D10a)-Nickase-hCMV-Puro
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6
pAAV-Guide-it-1 Vector (Linear)
pCLIP-Cas9(D10a)-Nickase-hCMV-tRFP
pHSN401
pUC119-gRNA
pAAV-Guide-it-Down Vector (Linear)
pCLIP-Cas9(D10a)-Nickase-hCMV-ZsGreen
pHsp70-Cas9
pUC57-sgRNA expression vector
pAAV-Guide-it-Up Vector
pCLIP-Cas9(D10a)-Nickase-TRE3G-Blast
pICH41308_hCas9
Puro-Cas9 donor
pAAVS1-BSD-DNR
pCLIP-Cas9(D10a)-Nickase-TRE3G-Puro
pICH47742_2x35S-5'UTR-hCas9(STOP)-NOST
pWJ66
pAAVS1-Puro-DNR
pCLIP-Cas9(D10a)-Nickase-TRE3G-tRFP
piCRg Entry
pWJ68
pAC2-dual-dCas9VP48-sgExpression
pCLIP-Cas9(D10a)-Nickase-TRE3G-ZsGreen
pICSL01009_AtU6p
pwtCas9-bacteria
pAc-sgRNA-Cas9
pCLIP-Cas9-Nuclease-EFS-Blast
pK7WGF2_hCas9
pX260
pAC149-pCR8-dCas9VP160
pCLIP-Cas9-Nuclease-EFS-Puro
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
pX330
pAC152-dual-dCas9VP64-sgExpression
pCLIP-Cas9-Nuclease-EFS-tRFP
pL-CRISPR.EFS.GFP
pX330A-1x2
pAC153-dual-dCas9VP96-sgExpression
pCLIP-Cas9-Nuclease-EFS-ZsGreen
pL-CRISPR.EFS.tRFP
pX330S-2
pAC154-dual-dCas9VP160-sgExpression
pCLIP-Cas9-Nuclease-hCMV-Blast
pL-CRISPR.SFFV.GFP
pX334
pAC155-pCR8-sgExpression
pCLIP-Cas9-Nuclease-hCMV-Puro
pL-CRISPR.SFFV.tRFP
pX335
pAC84-pCR8-dCas9
pCLIP-Cas9-Nuclease-hCMV-tRFP
pLenti-Cas9
PX551
pAC91-pmax-dCas9VP64
pCLIP-Cas9-Nuclease-hCMV-ZsGreen
pLenti-Cas9-IRES-Puro
PX552
pAC93-pmax-dCas9VP160
pCLIP-Cas9-Nuclease-TRE3G-Blast
pLenti-Cas9-P2A-tGFP
pX600
pAC94-pmax-dCas9VP160-2A-puro
pCLIP-Cas9-Nuclease-TRE3G-Puro
pLenti-Cas-Guide
pX601
pAC95-pmax-dCas9VP160-2A-neo
pCLIP-Cas9-Nuclease-TRE3G-tRFP
pLenti-EF1a-Cas9-IRES-Puro
pX602
pACT2-CAS9c
pCLIP-Cas9-Nuclease-TRE3G-ZsGreen
pLenti-Guide-Puro
PX851
pBS-Hsp70-Cas9
pCLIP-gRNA-EFS-Blast
pLKO.1-puro U6 sgRNA BfuAI stuffer
PX852
pCAG-T3-hCAS-pA
pCLIP-gRNA-EFS-Puro
pLSC-5
PX853
pCAG-T3-hCasD10A-pA
pCLIP-gRNA-EFS-tRFP
pLV hUbC-Cas9-T2A-GFP
PX854
pCas
pCLIP-gRNA-EFS-ZsGreen
pLV hUbC-dCas9 VP64-T2A-GFP
PX855
pCas-Guide-AAVS1
pCLIP-gRNA-hCMV-Blast
pLV hUbC-dCas9-T2A-GFP
PX856
pCas-Guide-CRISPRa
pCLIP-gRNA-hCMV-Puro
pLVX-hyg-sgRNA1 Vector (Linear)
pY010 (pcDNA3.1-hAsCpf1)
pCas-Guide-CRISPRi
pCLIP-gRNA-hCMV-tRFP
pLVX-puro-Cas9
pY016 (pcDNA3.1-hLbCpf1)
pCas-Guide-EF1a-CD4
pCLIP-gRNA-hCMV-ZsGreen
pLX-sgRNA
sgRNA(MS2) cloning backbone
pCas-Guide-EF1a-GFP
pCRCT
pMB66
SP-Cas9
pCas-Guide-Nickase
pCRISPomyces-1
pMB70
SP-dCas9-VPR
pCas-Guide-ROSA26
pCRISPomyces-2
pME-Cas9
VP12
pCas9
pCRISPR

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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