pMJ920

Doudna lab plasmid for mammalian cell expression of human codon-optimized Cas9 together with GFP.

Sequence Author: Doudna Lab / Addgene #42234

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SV40 NLS CMV enhancer SV40 NLS pMJ920 10,357 bp
Cas9
935 .. 5038  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
935 .. 5038  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
HA
5042 .. 5068  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
5042 .. 5068  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
SV40 NLS
5087 .. 5107  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5087 .. 5107  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5114 .. 5134  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5114 .. 5134  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
mEGFP
5174 .. 5890  =  717 bp
238 amino acids  =  26.7 kDa
2 segments
   Segment 1:  1a  
   5174 .. 5176  =  3 bp
   1 amino acid  =  117.1 Da
Product: enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)
mammalian codon-optimized
mEGFP
5174 .. 5890  =  717 bp
238 amino acids  =  26.7 kDa
2 segments
   Segment 2:  
   5177 .. 5890  =  714 bp
   237 amino acids  =  26.6 kDa
Product: enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)
mammalian codon-optimized
mEGFP
5174 .. 5890  =  717 bp
238 amino acids  =  26.7 kDa
2 segments
Product: enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)
mammalian codon-optimized
AmpR
9361 .. 10,221  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   9361 .. 10,152  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
9361 .. 10,221  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   10,153 .. 10,221  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
9361 .. 10,221  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
7062 .. 7856  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
7062 .. 7856  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
8602 .. 9190  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
8602 .. 9190  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
6223 .. 6651  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
6223 .. 6651  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
6665 .. 6995  =  331 bp
SV40 enhancer and early promoter
SV40 promoter
6665 .. 6995  =  331 bp
SV40 enhancer and early promoter
bGH poly(A) signal
5953 .. 6177  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
5953 .. 6177  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
8030 .. 8151  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
8030 .. 8151  =  122 bp
SV40 polyadenylation signal
AmpR promoter
10,222 .. 10,326  =  105 bp
AmpR promoter
10,222 .. 10,326  =  105 bp
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
6846 .. 6981  =  136 bp
SV40 origin of replication
SV40 ori
6846 .. 6981  =  136 bp
SV40 origin of replication
ORF:  7234 .. 7620  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  935 .. 5890  =  4956 bp
ORF:  1651 amino acids  =  189.8 kDa
ORF:  9491 .. 9757  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  6174 .. 6440  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  7062 .. 7856  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  875 .. 3454  =  2580 bp
ORF:  859 amino acids  =  90.3 kDa
ORF:  2284 .. 2577  =  294 bp
ORF:  97 amino acids  =  10.7 kDa
ORF:  2848 .. 3297  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
ORF:  5062 .. 5313  =  252 bp
ORF:  83 amino acids  =  9.1 kDa
ORF:  9361 .. 10,221  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  7371 .. 7907  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
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Download pMJ920.dna file

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