pDR274

Plasmid for in vitro transcription of a single guide RNA (sgRNA) using the T7 promoter. Archived by Addgene as DR274.

Sequence Author: Joung Lab / Addgene #42250

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No matches
DraI (199) BsaI (275) BsaI (300) DraI (484) pDR274 2147 bp
DraI  (199)
2 sites
T T T A A A A A A T T T
BsaI  (275)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsaI  (300)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
DraI  (484)
2 sites
T T T A A A A A A T T T
KanR
647 .. 1462  =  816 bp
271 amino acids  =  30.9 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
KanR
647 .. 1462  =  816 bp
271 amino acids  =  30.9 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
ori
1525 .. 2112  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1525 .. 2112  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
cat promoter
544 .. 646  =  103 bp
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
cat promoter
544 .. 646  =  103 bp
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
gRNA scaffold
204 .. 279  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
204 .. 279  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
T7Te terminator
1486 .. 1513  =  28 bp
phage T7 early transcription terminator
T7Te terminator
1486 .. 1513  =  28 bp
phage T7 early transcription terminator
T7 promoter
301 .. 319  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
301 .. 319  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  647 .. 1462  =  816 bp
ORF:  271 amino acids  =  30.9 kDa
ORF:  1449 .. 1850  =  402 bp
ORF:  133 amino acids  =  15.0 kDa
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Download pDR274.dna file

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