Peft-3_cas9-SV40_NLS_tbb-2 3'UTR

C. elegans plasmid for expressing nematode codon-optimized Cas9 with a synthetic intron. Use together with PU6::klp-12_sgRNA.

Sequence Author: Calarco Lab / Addgene #46168

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Peft-3::cas9-SV40_NLS::tbb-2 3'UTR 7799 bp
Cas9
1037 .. 5191  =  4155 bp
1368 amino acids  =  158.4 kDa
2 segments  =  4104 bp
Product: S. pyogenes Cas9, codon optimized for C. elegans with a synthetic intron
Cas9
1037 .. 5191  =  4155 bp
1368 amino acids  =  158.4 kDa
2 segments  =  4104 bp
   Segment 1:  
   1037 .. 3112  =  2076 bp
   692 amino acids  =  80.5 kDa
Product: S. pyogenes Cas9, codon optimized for C. elegans with a synthetic intron
Cas9
1037 .. 5191  =  4155 bp
1368 amino acids  =  158.4 kDa
2 segments  =  4104 bp
   Segment 2:  
   3164 .. 5191  =  2028 bp
   676 amino acids  =  78.0 kDa
Product: S. pyogenes Cas9, codon optimized for C. elegans with a synthetic intron
Cas9
1037 .. 5191  =  4155 bp
1368 amino acids  =  158.4 kDa
2 segments  =  4104 bp
Product: S. pyogenes Cas9, codon optimized for C. elegans with a synthetic intron
SV40 NLS
5204 .. 5224  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5204 .. 5224  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
AmpR
6739 .. 7599  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6739 .. 7530  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6739 .. 7599  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   7531 .. 7599  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6739 .. 7599  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
eft-3 promoter
402 .. 1001  =  600 bp
promoter for the C. elegans elongation factor 1α gene (Mitrovich and Anderson, 2000)
eft-3 promoter
402 .. 1001  =  600 bp
promoter for the C. elegans elongation factor 1α gene (Mitrovich and Anderson, 2000)
ori
5980 .. 6568  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5980 .. 6568  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
tbb-2 terminator
5233 .. 5556  =  324 bp
transcription terminator for the C. elegans tubulin beta-2 gene
tbb-2 terminator
5233 .. 5556  =  324 bp
transcription terminator for the C. elegans tubulin beta-2 gene
AmpR promoter
7600 .. 7704  =  105 bp
AmpR promoter
7600 .. 7704  =  105 bp
attB1
1010 .. 1034  =  25 bp
recombination site for the Gateway® BP reaction
attB1
1010 .. 1034  =  25 bp
recombination site for the Gateway® BP reaction
ORF:  1037 .. 3145  =  2109 bp
ORF:  702 amino acids  =  81.6 kDa
ORF:  3167 .. 5227  =  2061 bp
ORF:  686 amino acids  =  79.2 kDa
ORF:  6869 .. 7135  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  186 .. 461  =  276 bp
ORF:  91 amino acids  =  10.7 kDa
ORF:  250 .. 486  =  237 bp
ORF:  78 amino acids  =  8.7 kDa
ORF:  4201 .. 4446  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
ORF:  6739 .. 7599  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Download Peft-3_cas9-SV40_NLS_tbb-2 3'UTR.dna file

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