pX600

Zhang lab plasmid for expression of human codon-optimized Staphylococcus aureus Cas9. Also known as pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA.

Sequence Author: Zhang Lab / Addgene #61592

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ATG pX600 7101 bp
ATG
3366 .. 3368  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
3366 .. 3368  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 NLS
3372 .. 3392  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
3372 .. 3392  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SaCas9
3417 .. 6572  =  3156 bp
1052 amino acids  =  123.8 kDa
Product: Cas9 (Csn1) endonuclease from the Staphylococcus aureus Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SaCas9
3417 .. 6572  =  3156 bp
1052 amino acids  =  123.8 kDa
Product: Cas9 (Csn1) endonuclease from the Staphylococcus aureus Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
nucleoplasmin NLS
6573 .. 6620  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
nucleoplasmin NLS
6573 .. 6620  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
3xHA
6627 .. 6707  =  81 bp
27 amino acids  =  3.3 kDa
Product: three tandem HA epitope tags
3xHA
6627 .. 6707  =  81 bp
27 amino acids  =  3.3 kDa
Product: three tandem HA epitope tags
AmpR
821 .. 1681  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   821 .. 1612  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
821 .. 1681  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1613 .. 1681  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
821 .. 1681  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
62 .. 650  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
62 .. 650  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
2068 .. 2523  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
2068 .. 2523  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
2758 .. 3137  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2758 .. 3137  =  380 bp
human cytomegalovirus immediate early enhancer
bGH poly(A) signal
6741 .. 6948  =  208 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
6741 .. 6948  =  208 bp
bovine growth hormone polyadenylation signal
CMV promoter
3138 .. 3341  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
3138 .. 3341  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AAV2 ITR
2598 .. 2738  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
2598 .. 2738  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
6961 .. 7101  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
6961 .. 7101  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
1682 .. 1786  =  105 bp
AmpR promoter
1682 .. 1786  =  105 bp
ORF:  2543 .. 2776  =  234 bp
ORF:  77 amino acids  =  8.8 kDa
ORF:  4097 .. 4345  =  249 bp
ORF:  82 amino acids  =  9.8 kDa
ORF:  951 .. 1217  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3297 .. 6710  =  3414 bp
ORF:  1137 amino acids  =  133.4 kDa
ORF:  2500 .. 2724  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  3348 .. 6083  =  2736 bp
ORF:  911 amino acids  =  96.2 kDa
ORF:  821 .. 1681  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3677 .. 3916  =  240 bp
ORF:  79 amino acids  =  8.6 kDa
ORF:  4577 .. 5035  =  459 bp
ORF:  152 amino acids  =  17.7 kDa
ORF:  5399 .. 5701  =  303 bp
ORF:  100 amino acids  =  11.7 kDa
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