pSQT834

Plasmid for mammalian cell expression of the Csy4 CRISPR-associated endoribonuclease and wild-type S. pyogenes Cas9.

Sequence Author: Joung Lab / Addgene #53371

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SV40 NLS CMV enhancer pSQT834 9654 bp
Csy4
1769 .. 2329  =  561 bp
187 amino acids  =  21.4 kDa
Product: CRISPR-associated endoribonuclease responsible for pre-crRNA processing in Pseudomonas aeruginosa (Haurwitz et al., 2010)
Csy4
1769 .. 2329  =  561 bp
187 amino acids  =  21.4 kDa
Product: CRISPR-associated endoribonuclease responsible for pre-crRNA processing in Pseudomonas aeruginosa (Haurwitz et al., 2010)
T2A
2330 .. 2383  =  54 bp
18 amino acids  =  1.8 kDa
Product: 2A peptide from Thosea asigna virus capsid protein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
T2A
2330 .. 2383  =  54 bp
18 amino acids  =  1.8 kDa
Product: 2A peptide from Thosea asigna virus capsid protein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
Cas9
2384 .. 6487  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
2384 .. 6487  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
6494 .. 6514  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
6494 .. 6514  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
3xFLAG
6521 .. 6586  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags
3xFLAG
6521 .. 6586  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags
chimeric intron
662 .. 1670  =  1009 bp
chimera between introns from chicken β-actin and rabbit β-globin
chimeric intron
662 .. 1670  =  1009 bp
chimera between introns from chicken β-actin and rabbit β-globin
AmpR
8663 .. 9523  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   8663 .. 9454  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8663 .. 9523  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   9455 .. 9523  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8663 .. 9523  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
7904 .. 8492  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7904 .. 8492  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
4 .. 383  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
4 .. 383  =  380 bp
human cytomegalovirus immediate early enhancer
chicken β-actin promoter
386 .. 661  =  276 bp
chicken β-actin promoter
386 .. 661  =  276 bp
SV40 promoter
7330 .. 7525  =  196 bp
SV40 early promoter
SV40 promoter
7330 .. 7525  =  196 bp
SV40 early promoter
SV40 poly(A) signal
7531 .. 7665  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
7531 .. 7665  =  135 bp
SV40 polyadenylation signal
AmpR promoter
9524 .. 9628  =  105 bp
AmpR promoter
9524 .. 9628  =  105 bp
β-globin poly(A) signal
6741 .. 6796  =  56 bp
rabbit β-globin polyadenylation signal
β-globin poly(A) signal
6741 .. 6796  =  56 bp
rabbit β-globin polyadenylation signal
SV40 ori
7376 .. 7511  =  136 bp
SV40 origin of replication
SV40 ori
7376 .. 7511  =  136 bp
SV40 origin of replication
ORF:  1642 .. 2139  =  498 bp
ORF:  165 amino acids  =  16.8 kDa
ORF:  1355 .. 1627  =  273 bp
ORF:  90 amino acids  =  9.3 kDa
ORF:  1769 .. 6589  =  4821 bp
ORF:  1606 amino acids  =  185.5 kDa
ORF:  255 .. 734  =  480 bp
ORF:  159 amino acids  =  17.1 kDa
ORF:  8793 .. 9059  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  802 .. 1533  =  732 bp
ORF:  243 amino acids  =  26.3 kDa
ORF:  4348 .. 4635  =  288 bp
ORF:  95 amino acids  =  10.5 kDa
ORF:  5497 .. 5742  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
ORF:  9420 .. 8  =  243 bp
ORF:  80 amino acids  =  9.3 kDa
ORF:  843 .. 1346  =  504 bp
ORF:  167 amino acids  =  16.7 kDa
ORF:  1706 .. 2014  =  309 bp
ORF:  102 amino acids  =  11.2 kDa
ORF:  6470 .. 6727  =  258 bp
ORF:  85 amino acids  =  9.1 kDa
ORF:  8663 .. 9523  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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