p414-TEF1p-Cas9-CYC1t

Yeast centromeric plasmid for expressing human codon-optimized Cas9 with the constitutive TEF1 promoter.

Sequence Author: Church Lab / Addgene #43802

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p414-TEF1p-Cas9-CYC1t 9524 bp
Cas9
3222 .. 7325  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
3222 .. 7325  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
7338 .. 7358  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
7338 .. 7358  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
AmpR
714 .. 1574  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   714 .. 782  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
714 .. 1574  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   783 .. 1574  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
714 .. 1574  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
TRP1
8383 .. 9057  =  675 bp
224 amino acids  =  24.1 kDa
Product: phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis
yeast auxotrophic marker
TRP1
8383 .. 9057  =  675 bp
224 amino acids  =  24.1 kDa
Product: phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis
yeast auxotrophic marker
ori
1745 .. 2333  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1745 .. 2333  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CEN/ARS
69 .. 572  =  504 bp
S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence
CEN/ARS
69 .. 572  =  504 bp
S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence
f1 ori
7829 .. 8284  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
7829 .. 8284  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
TEF1 promoter
2792 .. 3190  =  399 bp
promoter for EF-1α
TEF1 promoter
2792 .. 3190  =  399 bp
promoter for EF-1α
TRP1 promoter
9058 .. 9338  =  281 bp
TRP1 promoter
9058 .. 9338  =  281 bp
CYC1 terminator
7374 .. 7621  =  248 bp
transcription terminator for CYC1
CYC1 terminator
7374 .. 7621  =  248 bp
transcription terminator for CYC1
AmpR promoter
609 .. 713  =  105 bp
AmpR promoter
609 .. 713  =  105 bp
ORF:  7490 .. 7813  =  324 bp
ORF:  107 amino acids  =  12.0 kDa
ORF:  714 .. 1574  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3222 .. 7361  =  4140 bp
ORF:  1379 amino acids  =  159.7 kDa
ORF:  3201 .. 3701  =  501 bp
ORF:  166 amino acids  =  18.6 kDa
ORF:  4626 .. 5144  =  519 bp
ORF:  172 amino acids  =  20.1 kDa
ORF:  5208 .. 5741  =  534 bp
ORF:  177 amino acids  =  19.9 kDa
ORF:  6735 .. 7268  =  534 bp
ORF:  177 amino acids  =  20.0 kDa
ORF:  8526 .. 8807  =  282 bp
ORF:  93 amino acids  =  10.8 kDa
ORF:  1178 .. 1444  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  8383 .. 9057  =  675 bp
ORF:  224 amino acids  =  24.1 kDa
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