MSP1101

Vector for expressing the S. pyogenes Cas9 VRER mutant, which has an altered PAM recognition specificity.

Sequence Author: Joung Lab / Addgene #65773

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SV40 NLS MSP1101 7614 bp
Cas9 VRER
409 .. 4512  =  4104 bp
1368 amino acids  =  158.6 kDa
9 segments
   Segment 1:  
   409 .. 3810  =  3402 bp
   1134 amino acids  =  131.7 kDa
Product: Streptococcus pyogenes Cas9 endonuclease with the D1135V/G1218R/R1335E/T1337R mutations
has an altered recognition specificity for the protospacer adjacent motif (PAM) (Kleinstiver et al., 2015)
Cas9 VRER
409 .. 4512  =  4104 bp
1368 amino acids  =  158.6 kDa
9 segments
   Segment 2:  
   3811 .. 3813  =  3 bp
   1 amino acid  =  117.1 Da
Product: Streptococcus pyogenes Cas9 endonuclease with the D1135V/G1218R/R1335E/T1337R mutations
has an altered recognition specificity for the protospacer adjacent motif (PAM) (Kleinstiver et al., 2015)
Cas9 VRER
409 .. 4512  =  4104 bp
1368 amino acids  =  158.6 kDa
9 segments
   Segment 3:  
   3814 .. 4059  =  246 bp
   82 amino acids  =  9.3 kDa
Product: Streptococcus pyogenes Cas9 endonuclease with the D1135V/G1218R/R1335E/T1337R mutations
has an altered recognition specificity for the protospacer adjacent motif (PAM) (Kleinstiver et al., 2015)
Cas9 VRER
409 .. 4512  =  4104 bp
1368 amino acids  =  158.6 kDa
9 segments
   Segment 4:  
   4060 .. 4062  =  3 bp
   1 amino acid  =  174.2 Da
Product: Streptococcus pyogenes Cas9 endonuclease with the D1135V/G1218R/R1335E/T1337R mutations
has an altered recognition specificity for the protospacer adjacent motif (PAM) (Kleinstiver et al., 2015)
Cas9 VRER
409 .. 4512  =  4104 bp
1368 amino acids  =  158.6 kDa
9 segments
   Segment 5:  
   4063 .. 4410  =  348 bp
   116 amino acids  =  13.5 kDa
Product: Streptococcus pyogenes Cas9 endonuclease with the D1135V/G1218R/R1335E/T1337R mutations
has an altered recognition specificity for the protospacer adjacent motif (PAM) (Kleinstiver et al., 2015)
Cas9 VRER
409 .. 4512  =  4104 bp
1368 amino acids  =  158.6 kDa
9 segments
   Segment 6:  
   4411 .. 4413  =  3 bp
   1 amino acid  =  147.1 Da
Product: Streptococcus pyogenes Cas9 endonuclease with the D1135V/G1218R/R1335E/T1337R mutations
has an altered recognition specificity for the protospacer adjacent motif (PAM) (Kleinstiver et al., 2015)
Cas9 VRER
409 .. 4512  =  4104 bp
1368 amino acids  =  158.6 kDa
9 segments
   Segment 7:  
   4414 .. 4416  =  3 bp
   1 amino acid  =  181.2 Da
Product: Streptococcus pyogenes Cas9 endonuclease with the D1135V/G1218R/R1335E/T1337R mutations
has an altered recognition specificity for the protospacer adjacent motif (PAM) (Kleinstiver et al., 2015)
Cas9 VRER
409 .. 4512  =  4104 bp
1368 amino acids  =  158.6 kDa
9 segments
   Segment 8:  
   4417 .. 4419  =  3 bp
   1 amino acid  =  174.2 Da
Product: Streptococcus pyogenes Cas9 endonuclease with the D1135V/G1218R/R1335E/T1337R mutations
has an altered recognition specificity for the protospacer adjacent motif (PAM) (Kleinstiver et al., 2015)
Cas9 VRER
409 .. 4512  =  4104 bp
1368 amino acids  =  158.6 kDa
9 segments
   Segment 9:  
   4420 .. 4512  =  93 bp
   31 amino acids  =  3.4 kDa
Product: Streptococcus pyogenes Cas9 endonuclease with the D1135V/G1218R/R1335E/T1337R mutations
has an altered recognition specificity for the protospacer adjacent motif (PAM) (Kleinstiver et al., 2015)
Cas9 VRER
409 .. 4512  =  4104 bp
1368 amino acids  =  158.6 kDa
9 segments
Product: Streptococcus pyogenes Cas9 endonuclease with the D1135V/G1218R/R1335E/T1337R mutations
has an altered recognition specificity for the protospacer adjacent motif (PAM) (Kleinstiver et al., 2015)
SV40 NLS
4519 .. 4539  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4519 .. 4539  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
3xFLAG
4546 .. 4611  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
3xFLAG
4546 .. 4611  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
AmpR
6131 .. 6991  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6131 .. 6922  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6131 .. 6991  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6923 .. 6991  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6131 .. 6991  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
5372 .. 5960  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5372 .. 5960  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
7366 .. 131  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
7366 .. 131  =  380 bp
human cytomegalovirus immediate early enhancer
bGH poly(A) signal
4675 .. 4899  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
4675 .. 4899  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
132 .. 335  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
132 .. 335  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
6992 .. 7096  =  105 bp
AmpR promoter
6992 .. 7096  =  105 bp
T7 promoter
377 .. 395  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
377 .. 395  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  409 .. 4623  =  4215 bp
ORF:  1404 amino acids  =  162.6 kDa
ORF:  6261 .. 6527  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2373 .. 2660  =  288 bp
ORF:  95 amino acids  =  10.5 kDa
ORF:  3522 .. 3767  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
ORF:  6131 .. 6991  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Download MSP1101.dna file

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