pET-Cas9-NLS-6xHis

Bacterial vector for regulated expression of S. pyogenes Cas9 with a C-terminal nuclear localization signal and 6xHis tag.

Sequence Author: Liu Lab / Addgene #62933

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
T7 promoter RBS 6xHis pET-Cas9-NLS-6xHis 9265 bp
Cas9
71 .. 4174  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
71 .. 4174  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
4175 .. 4195  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4175 .. 4195  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
6xHis
4205 .. 4222  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
4205 .. 4222  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
lacI
7776 .. 8858  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
7776 .. 8858  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
AmpR
4961 .. 5821  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4961 .. 5752  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4961 .. 5821  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5753 .. 5821  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4961 .. 5821  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
5995 .. 6583  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5995 .. 6583  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
4391 .. 4846  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4391 .. 4846  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
rop
7013 .. 7204  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein, which maintains plasmids at low copy number
rop
7013 .. 7204  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein, which maintains plasmids at low copy number
AmpR promoter
5822 .. 5914  =  93 bp
AmpR promoter
5822 .. 5914  =  93 bp
lacI promoter
8859 .. 8936  =  78 bp
lacI promoter
8859 .. 8936  =  78 bp
T7 terminator
4307 .. 4354  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
4307 .. 4354  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
lac operator
3 .. 27  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
3 .. 27  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RBS
42 .. 64  =  23 bp
efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
RBS
42 .. 64  =  23 bp
efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
T7 promoter
9249 .. 2  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
9249 .. 2  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  9034 .. 20  =  252 bp
ORF:  83 amino acids  =  8.6 kDa
ORF:  71 .. 4225  =  4155 bp
ORF:  1384 amino acids  =  160.5 kDa
ORF:  7742 .. 7993  =  252 bp
ORF:  83 amino acids  =  9.1 kDa
ORF:  8897 .. 9160  =  264 bp
ORF:  87 amino acids  =  9.5 kDa
ORF:  5091 .. 5357  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  7236 .. 7604  =  369 bp
ORF:  122 amino acids  =  14.2 kDa
ORF:  4961 .. 5821  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  7013 .. 7237  =  225 bp
ORF:  74 amino acids  =  8.5 kDa
ORF:  7756 .. 8019  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  8608 .. 9108  =  501 bp
ORF:  166 amino acids  =  17.5 kDa
ORF:  7776 .. 8735  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
Click here to try SnapGene

Download pET-Cas9-NLS-6xHis.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.