pwtCas9-bacteria

Bacterial plasmid for tetracycline-inducible expression of catalytically active wild-type S. pyogenes Cas9.

Sequence Author: Qi Lab / Addgene #44250

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pwtCas9-bacteria 6932 bp
Cas9
740 .. 4846  =  4107 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
740 .. 4846  =  4107 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
AmpR
5951 .. 6811  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5951 .. 6742  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5951 .. 6811  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6743 .. 6811  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5951 .. 6811  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
TetR
7 .. 630  =  624 bp
207 amino acids  =  23.4 kDa
Product: tetracycline repressor TetR
TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline.
TetR
7 .. 630  =  624 bp
207 amino acids  =  23.4 kDa
Product: tetracycline repressor TetR
TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline.
ori
5151 .. 5739  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5151 .. 5739  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
AmpR promoter
6812 .. 6916  =  105 bp
AmpR promoter
6812 .. 6916  =  105 bp
lambda t0 terminator
5827 .. 5921  =  95 bp
transcription terminator from phage lambda
lambda t0 terminator
5827 .. 5921  =  95 bp
transcription terminator from phage lambda
rrnB T1 terminator
4870 .. 4941  =  72 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
4870 .. 4941  =  72 bp
transcription terminator T1 from the E. coli rrnB gene
tetR/tetA promoters
649 .. 704  =  56 bp
overlapping promoters for bacterial tetR and tetA
tetR/tetA promoters
649 .. 704  =  56 bp
overlapping promoters for bacterial tetR and tetA
T7Te terminator
4957 .. 4984  =  28 bp
phage T7 early transcription terminator
T7Te terminator
4957 .. 4984  =  28 bp
phage T7 early transcription terminator
RBS
722 .. 733  =  12 bp
strong bacterial ribosome binding site (Elowitz and Leibler, 2000)
RBS
722 .. 733  =  12 bp
strong bacterial ribosome binding site (Elowitz and Leibler, 2000)
tet operator
685 .. 703  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
685 .. 703  =  19 bp
bacterial operator O2 for the tetR and tetA genes
ORF:  740 .. 4846  =  4107 bp
ORF:  1368 amino acids  =  158.4 kDa
ORF:  6081 .. 6347  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5951 .. 6811  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  7 .. 633  =  627 bp
ORF:  208 amino acids  =  23.5 kDa
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