pdCas9 BFP-humanized

Retroviral vector encoding human codon-optimized catalytically dead dCas9 fused to TagBFP, for visualizing genomic loci using a small guide RNA (sgRNA).

Sequence Author: Qi Lab / Addgene #44247

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
pdCas9::BFP-humanized 11,170 bp
dCas9
1423 .. 5526  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 1:  
   1423 .. 1449  =  27 bp
   9 amino acids  =  1.1 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1423 .. 5526  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 2:  
   1450 .. 1452  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1423 .. 5526  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 3:  
   1453 .. 3939  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1423 .. 5526  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 4:  
   3940 .. 3942  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1423 .. 5526  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 5:  
   3943 .. 5526  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1423 .. 5526  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
SV40 NLS
5536 .. 5556  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5536 .. 5556  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
TagBFP
5566 .. 6261  =  696 bp
232 amino acids  =  26.2 kDa
Product: monomeric blue fluorescent protein
mammalian codon-optimized
TagBFP
5566 .. 6261  =  696 bp
232 amino acids  =  26.2 kDa
Product: monomeric blue fluorescent protein
mammalian codon-optimized
SV40 NLS
6271 .. 6291  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
6271 .. 6291  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
AmpR
9376 .. 10,236  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   9376 .. 10,167  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
9376 .. 10,236  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   10,168 .. 10,236  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
9376 .. 10,236  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
PuroR
6833 .. 7432  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
6833 .. 7432  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
ori
8617 .. 9205  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
8617 .. 9205  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
5' LTR
11,169 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem cell virus
5' LTR
11,169 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem cell virus
3' LTR
7532 .. 8046  =  515 bp
3' long terminal repeat from murine embryonic stem cell virus
3' LTR
7532 .. 8046  =  515 bp
3' long terminal repeat from murine embryonic stem cell virus
PGK promoter
6313 .. 6812  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
6313 .. 6812  =  500 bp
mouse phosphoglycerate kinase 1 promoter
gag (truncated)
987 .. 1403  =  417 bp
truncated MMLV gag gene lacking the start codon
gag (truncated)
987 .. 1403  =  417 bp
truncated MMLV gag gene lacking the start codon
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell virus
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell virus
AmpR promoter
10,237 .. 10,341  =  105 bp
AmpR promoter
10,237 .. 10,341  =  105 bp
ORF:  1423 .. 6303  =  4881 bp
ORF:  1626 amino acids  =  187.2 kDa
ORF:  5273 .. 5557  =  285 bp
ORF:  94 amino acids  =  10.0 kDa
ORF:  6593 .. 7432  =  840 bp
ORF:  279 amino acids  =  30.1 kDa
ORF:  9506 .. 9772  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  10,622 .. 10,918  =  297 bp
ORF:  98 amino acids  =  11.7 kDa
ORF:  362 .. 637  =  276 bp
ORF:  91 amino acids  =  9.9 kDa
ORF:  674 .. 934  =  261 bp
ORF:  86 amino acids  =  9.5 kDa
ORF:  6758 .. 7483  =  726 bp
ORF:  241 amino acids  =  24.8 kDa
ORF:  3043 .. 3516  =  474 bp
ORF:  157 amino acids  =  17.4 kDa
ORF:  4828 .. 5214  =  387 bp
ORF:  128 amino acids  =  14.5 kDa
ORF:  5509 .. 6420  =  912 bp
ORF:  303 amino acids  =  31.7 kDa
ORF:  9376 .. 10,236  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3336 .. 3704  =  369 bp
ORF:  122 amino acids  =  14.1 kDa
ORF:  10,686 .. 10,916  =  231 bp
ORF:  76 amino acids  =  7.5 kDa
Click here to try SnapGene

Download pdCas9 BFP-humanized.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.