pROSA26-Puro-DNR

Mammalian donor vector with a puromycin resistance marker for transgene integration at the ROSA26 locus in the mouse genome. Use together with pCas‑Guide-ROSA26.

Sequence Author: OriGene

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HomePlasmidsCRISPR PlasmidspROSA26-Puro-DNR
BsrGI (7805) MfeI (7149) BbvCI (6872) AleI (6646) FseI (6556) PmeI (6546) PspXI (6468) NotI (6462) MluI (6453) BmtI (6445) NheI (6441) AscI (6416) MreI - SgrAI (6412) AsiSI (6410) KpnI (6388) Acc65I (6384) BamHI (6378) AccI (6372) SalI (6371) EcoRI (6365) SacI (6312) Eco53kI (6310) NcoI (6104) SnaBI (6084) NdeI (5978) CMV enhancer PpuMI (5269) BfuAI - BspMI (5173) PflFI - Tth111I (5104) BstEII (5008) BstAPI (4579) BspDI - ClaI (4491) PflMI (4146) ApaI (3924) BspQI - SapI (319) PciI (435) SbfI (3787) PspOMI (3920) pROSA26-Puro-DNR 7810 bp
BsrGI  (7805)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
MfeI  (7149)
1 site		 C A A T T G G T T A A C
BbvCI  (6872)
1 site		 C C T C A G C G G A G T C G
AleI  (6646)
1 site		 C A C N N N N G T G G T G N N N N C A C
FseI  (6556)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
PmeI  (6546)
1 site		 G T T T A A A C C A A A T T T G
PspXI  (6468)
1 site		 V C T C G A G B B G A G C T C V
NotI  (6462)
1 site		 G C G G C C G C C G C C G G C G
MluI  (6453)
1 site		 A C G C G T T G C G C A
BmtI  (6445)
1 site		 G C T A G C C G A T C G
NheI  (6441)
1 site		 G C T A G C C G A T C G
AscI  (6416)
1 site		 G G C G C G C C C C G C G C G G
MreI  (6412)
1 site		 C G C C G G C G G C G G C C G C
SgrAI  (6412)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AsiSI  (6410)
1 site		 G C G A T C G C C G C T A G C G
KpnI  (6388)
1 site		 G G T A C C C C A T G G
Acc65I  (6384)
1 site		 G G T A C C C C A T G G
BamHI  (6378)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AccI  (6372)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (6371)
1 site		 G T C G A C C A G C T G
EcoRI  (6365)
1 site		 G A A T T C C T T A A G
SacI  (6312)
1 site		 G A G C T C C T C G A G
Eco53kI  (6310)
1 site		 G A G C T C C T C G A G
NcoI  (6104)
1 site		 C C A T G G G G T A C C
SnaBI  (6084)
1 site		 T A C G T A A T G C A T
NdeI  (5978)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
PpuMI  (5269)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
BfuAI  (5173)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (5173)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PflFI  (5104)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5104)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BstEII  (5008)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstAPI  (4579)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
BspDI  (4491)
1 site		 A T C G A T T A G C T A
ClaI  (4491)
1 site		 A T C G A T T A G C T A
PflMI  (4146)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
ApaI  (3924)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BspQI  (319)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (319)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (435)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
SbfI  (3787)
1 site		 C C T G C A G G G G A C G T C C
PspOMI  (3920)
1 site		 G G G C C C C C C G G G
AmpR
1255 .. 2115  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1255 .. 2046  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1255 .. 2115  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2047 .. 2115  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1255 .. 2115  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
HA-R
7161 .. 7810  =  650 bp
right homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)
HA-R
7161 .. 7810  =  650 bp
right homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)
HA-L
3271 .. 3919  =  649 bp
left homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)
HA-L
3271 .. 3919  =  649 bp
left homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)
PuroR
4549 .. 5148  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
4549 .. 5148  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
ori
496 .. 1084  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
496 .. 1084  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
PGK promoter
5188 .. 5687  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
5188 .. 5687  =  500 bp
mouse phosphoglycerate kinase 1 promoter
hGH poly(A) signal
6589 .. 7065  =  477 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
6589 .. 7065  =  477 bp
human growth hormone polyadenylation signal
f1 ori
2502 .. 2957  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
2502 .. 2957  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
5805 .. 6108  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
5805 .. 6108  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
6109 .. 6312  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
6109 .. 6312  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
MCS
6364 .. 6558  =  195 bp
multiple cloning site
MCS
6364 .. 6558  =  195 bp
multiple cloning site
AmpR promoter
2116 .. 2220  =  105 bp
AmpR promoter
2116 .. 2220  =  105 bp
T7 promoter
6338 .. 6356  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
6338 .. 6356  =  19 bp
promoter for bacteriophage T7 RNA polymerase
Myc
6471 .. 6500  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
Myc
6471 .. 6500  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
FLAG
6519 .. 6542  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
6519 .. 6542  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
ORF:  2977 .. 3405  =  429 bp
ORF:  142 amino acids  =  15.8 kDa
ORF:  4498 .. 5175  =  678 bp
ORF:  225 amino acids  =  23.1 kDa
ORF:  1385 .. 1651  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  4460 .. 5407  =  948 bp
ORF:  315 amino acids  =  34.8 kDa
ORF:  1255 .. 2115  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  4549 .. 5148  =  600 bp
ORF:  199 amino acids  =  21.5 kDa
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Individual Sequences & Maps

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pME-Cas9-T2A-GFP
3xFLAG-dCas9 pMXs-neo
pCas9D10A_GFP
pCS2+hSpCas9
pMJ806
3xFLAG-dCas9 pMXs-puro
pCas-Guide
pCS2-Cas9
pMJ841
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pcDNA-dCas9
pCS2-nCas9n
pMJ920
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CRISPRi library backbone
pcDNA-dCas9-p300 Core (D1399Y)
pdCas9
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pcDNA-dCas9-VP64
pdCas9-bacteria
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eSpCas9(1.1)
pcDNA3.1-CibN-dCas9-CibN
pdCas9-humanized
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GeneArt CRISPR Nuclease Vector - CD4 (linearized)
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pNW3
GeneArt CRISPR Nuclease Vector - OFP (linearized)
pCFD2-dU6-2gRNA
pDCC6
pRGE31
gRNA_AAVS1-T1
pCFD3-dU6-3gRNA
pDD162 (Peft-3_Cas9 + Empty sgRNA)
pRGEB31
gRNA_AAVS1-T2
pCFD4-U6-1_U6-3tandemgRNAs
pDR274
pRGEB32
gRNA_Cloning Vector
pCLIP-All-EFS-Ametrine
Peft-3_cas9-SV40_NLS_tbb-2 3'UTR
pROSA26-Puro-DNR
gRNA_GFP-T1
pCLIP-All-EFS-Blast
pET-28b-Cas9-His
pRPR1_gRNA_handle_RPR1t
gRNA_GFP-T2
pCLIP-All-EFS-Puro
pET-Cas9-6xHis
pSECC
HBT-pcoCas9
pCLIP-All-EFS-tRFP
pET-Cas9-NLS-6xHis
pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
hCas9
pCLIP-All-EFS-ZsGreen
pET-NLS-Cas9-6xHis
pSLQ1658-dCas9-EGFP
hCas9_D10A
pCLIP-All-hCMV-Blast
pET28a Cas9-Cys
pSpCas9(BB)-2A-GFP (PX458)
JDS246
pCLIP-All-hCMV-Puro
pFGC-pcoCas9
pSpCas9(BB)-2A-Puro (PX459) V2.0
lenti dCAS-VP64_Blast
pCLIP-All-hCMV-tRFP
pGL3-U6-sgRNA-PGK-puromycin
pSpCas9n(BB)-2A-GFP (PX461)
lenti MS2-P65-HSF1_Hygro
pCLIP-All-hCMV-ZsGreen
pgRNA-bacteria
pSpCas9n(BB)-2A-Puro (PX462) V2.0
lenti sgRNA(MS2)_zeo backbone
pCLIP-All-Nickase(D10A)-EFS-Blast
pgRNA-humanized
pSpCas9n(BB) (PX460)
lentiCas9-Blast
pCLIP-All-Nickase(D10A)-EFS-Puro
pGuide
pSpCas9 (PX165)
lentiCas9-EGFP
pCLIP-All-Nickase(D10A)-EFS-tRFP
pGuide-it-sgRNA1 Vector (Linear)
pSPgRNA
lentiCas9n(D10A)-Blast
pCLIP-All-Nickase(D10A)-EFS-ZsGreen
pGuide-it-tdTomato Vector (Linear)
pSQT834
lentiCRISPR v2
pCLIP-All-Nickase(D10A)-hCMV-Puro
pGuide-it-ZsGreen1 Vector (Linear)
pSQT1313
lentiGuide-Puro
pCLIP-All-Nickase(D10A)-hCMV-tRFP
pHAGE EF1-alpha dCas9-KRAB
pST1374-N-NLS-flag-linker-Cas9-D10A
LSL-Cas9-Rosa26TV
pCLIP-All-Nickase(D10A)-hCMV-ZsGreen
pHAGE EF1-alpha dCas9-VP64
pST1374-N-NLS-flag-linker-Cas9-H840A
M-SPcas
pCLIP-All-Nickase(D10A)-TRE3G-Blast
pHAGE TRE dCas9
pST1374-NLS-flag-linker-Cas9
M-ST1cas
pCLIP-All-Nickase(D10A)-TRE3G-Puro
pHAGE TRE dCas9-KRAB
pT3TS-nCas9n
MLM3613
pCLIP-All-Nickase(D10A)-TRE3G-tRFP
pHAGE TRE dCas9-VP64
pT7-Cas9
MLM3636
pCLIP-All-Nickase(D10A)-TRE3G-ZsGreen
pHAGE-TO-dCas9-3XGFP
pT7-Cas9-Nickase
MLM3639
pCLIP-All-TRE3G-Blast
pHAGE-TO-dCas9-3XmCherry
pT7-gRNA
MS2-P65-HSF1_GFP
pCLIP-All-TRE3G-Puro
pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2
pT7-Guide-IVT
MSP1101
pCLIP-All-TRE3G-tRFP
pHAGE-TO-nmdCas9-3XGFP
pTDH3-dCas9
MSP2133
pCLIP-All-TRE3G-ZsGreen
pHAGE-TO-nmdCas9-3XmCherry
pTDH3-dCas9-Mxi1
MSP2135
pCLIP-Cas9(D10a)-Nickase-EFS-Puro
pHR-SFFV-dCas9-BFP
pTOL-hCMV-TET3G-Hygro Transactivator
MSP469
pCLIP-Cas9(D10a)-Nickase-EFS-Blast
pHR-SFFV-dCas9-BFP-KRAB
pTPGI_dCas9_VP64
p3s-Cas9HC
pCLIP-Cas9(D10a)-Nickase-EFS-tRFP
pHR-SFFV-KRAB-dCas9-P2A-mCherry
pU6-BbsI-chiRNA
p414-TEF1p-Cas9-CYC1t
pCLIP-Cas9(D10a)-Nickase-EFS-ZsGreen
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
PU6_klp-12_sgRNA
p415-GalL-Cas9-CYC1t
pCLIP-Cas9(D10a)-Nickase-hCMV-Blast
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
p426-SNR52p-gRNA.CAN1.Y-SUP4t
pCLIP-Cas9(D10a)-Nickase-hCMV-Puro
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6
pAAV-Guide-it-1 Vector (Linear)
pCLIP-Cas9(D10a)-Nickase-hCMV-tRFP
pHSN401
pUC119-gRNA
pAAV-Guide-it-Down Vector (Linear)
pCLIP-Cas9(D10a)-Nickase-hCMV-ZsGreen
pHsp70-Cas9
pUC57-sgRNA expression vector
pAAV-Guide-it-Up Vector
pCLIP-Cas9(D10a)-Nickase-TRE3G-Blast
pICH41308_hCas9
Puro-Cas9 donor
pAAVS1-BSD-DNR
pCLIP-Cas9(D10a)-Nickase-TRE3G-Puro
pICH47742_2x35S-5'UTR-hCas9(STOP)-NOST
pWJ66
pAAVS1-Puro-DNR
pCLIP-Cas9(D10a)-Nickase-TRE3G-tRFP
piCRg Entry
pWJ68
pAC2-dual-dCas9VP48-sgExpression
pCLIP-Cas9(D10a)-Nickase-TRE3G-ZsGreen
pICSL01009_AtU6p
pwtCas9-bacteria
pAc-sgRNA-Cas9
pCLIP-Cas9-Nuclease-EFS-Blast
pK7WGF2_hCas9
pX260
pAC149-pCR8-dCas9VP160
pCLIP-Cas9-Nuclease-EFS-Puro
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
pX330
pAC152-dual-dCas9VP64-sgExpression
pCLIP-Cas9-Nuclease-EFS-tRFP
pL-CRISPR.EFS.GFP
pX330A-1x2
pAC153-dual-dCas9VP96-sgExpression
pCLIP-Cas9-Nuclease-EFS-ZsGreen
pL-CRISPR.EFS.tRFP
pX330S-2
pAC154-dual-dCas9VP160-sgExpression
pCLIP-Cas9-Nuclease-hCMV-Blast
pL-CRISPR.SFFV.GFP
pX334
pAC155-pCR8-sgExpression
pCLIP-Cas9-Nuclease-hCMV-Puro
pL-CRISPR.SFFV.tRFP
pX335
pAC84-pCR8-dCas9
pCLIP-Cas9-Nuclease-hCMV-tRFP
pLenti-Cas9
PX551
pAC91-pmax-dCas9VP64
pCLIP-Cas9-Nuclease-hCMV-ZsGreen
pLenti-Cas9-IRES-Puro
PX552
pAC93-pmax-dCas9VP160
pCLIP-Cas9-Nuclease-TRE3G-Blast
pLenti-Cas9-P2A-tGFP
pX600
pAC94-pmax-dCas9VP160-2A-puro
pCLIP-Cas9-Nuclease-TRE3G-Puro
pLenti-Cas-Guide
pX601
pAC95-pmax-dCas9VP160-2A-neo
pCLIP-Cas9-Nuclease-TRE3G-tRFP
pLenti-EF1a-Cas9-IRES-Puro
pX602
pACT2-CAS9c
pCLIP-Cas9-Nuclease-TRE3G-ZsGreen
pLenti-Guide-Puro
PX851
pBS-Hsp70-Cas9
pCLIP-gRNA-EFS-Blast
pLKO.1-puro U6 sgRNA BfuAI stuffer
PX852
pCAG-T3-hCAS-pA
pCLIP-gRNA-EFS-Puro
pLSC-5
PX853
pCAG-T3-hCasD10A-pA
pCLIP-gRNA-EFS-tRFP
pLV hUbC-Cas9-T2A-GFP
PX854
pCas
pCLIP-gRNA-EFS-ZsGreen
pLV hUbC-dCas9 VP64-T2A-GFP
PX855
pCas-Guide-AAVS1
pCLIP-gRNA-hCMV-Blast
pLV hUbC-dCas9-T2A-GFP
PX856
pCas-Guide-CRISPRa
pCLIP-gRNA-hCMV-Puro
pLVX-hyg-sgRNA1 Vector (Linear)
pY010 (pcDNA3.1-hAsCpf1)
pCas-Guide-CRISPRi
pCLIP-gRNA-hCMV-tRFP
pLVX-puro-Cas9
pY016 (pcDNA3.1-hLbCpf1)
pCas-Guide-EF1a-CD4
pCLIP-gRNA-hCMV-ZsGreen
pLX-sgRNA
sgRNA(MS2) cloning backbone
pCas-Guide-EF1a-GFP
pCRCT
pMB66
SP-Cas9
pCas-Guide-Nickase
pCRISPomyces-1
pMB70
SP-dCas9-VPR
pCas-Guide-ROSA26
pCRISPomyces-2
pME-Cas9
VP12
pCas9
pCRISPR

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