pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

CRISPR activation vector encoding TagBFP plus catalytically inactive dCas9 fused to 24 copies of an optimized GCN4_v4 peptide.

Sequence Author: Vale Lab / Addgene #60910

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HomePlasmidsCRISPR PlasmidspHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
SV40 NLS SV40 NLS SV40 NLS SV40 NLS ATG pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE 14,911 bp
ATG
7732 .. 7734  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
7732 .. 7734  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 NLS
7735 .. 7755  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
7735 .. 7755  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 1:  
   7810 .. 7836  =  27 bp
   9 amino acids  =  1.1 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 2:  
   7837 .. 7839  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 3:  
   7840 .. 10,326  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 4:  
   10,327 .. 10,329  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 5:  
   10,330 .. 11,913  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
7810 .. 11,913  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
HA
11,917 .. 11,943  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
11,917 .. 11,943  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
SV40 NLS
11,962 .. 11,982  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
11,962 .. 11,982  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
11,989 .. 12,009  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
11,989 .. 12,009  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 1:  GCN4_v4  
   12,088 .. 12,144  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 2:  
   12,145 .. 12,159  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 3:  GCN4_v4  
   12,160 .. 12,216  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 4:  
   12,217 .. 12,231  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 5:  GCN4_v4  
   12,232 .. 12,288  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 6:  
   12,289 .. 12,303  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 7:  GCN4_v4  
   12,304 .. 12,360  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 8:  
   12,361 .. 12,375  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 9:  GCN4_v4  
   12,376 .. 12,432  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 10:  
   12,433 .. 12,447  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 11:  GCN4_v4  
   12,448 .. 12,504  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 12:  
   12,505 .. 12,519  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 13:  GCN4_v4  
   12,520 .. 12,576  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 14:  
   12,577 .. 12,591  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 15:  GCN4_v4  
   12,592 .. 12,648  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 16:  
   12,649 .. 12,663  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 17:  GCN4_v4  
   12,664 .. 12,720  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 18:  
   12,721 .. 12,735  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 19:  GCN4_v4  
   12,736 .. 12,792  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 20:  
   12,793 .. 12,807  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 21:  GCN4_v4  
   12,808 .. 12,864  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 22:  
   12,865 .. 12,879  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 23:  GCN4_v4  
   12,880 .. 12,936  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 24:  
   12,937 .. 12,951  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 25:  GCN4_v4  
   12,952 .. 13,008  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 26:  
   13,009 .. 13,023  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 27:  GCN4_v4  
   13,024 .. 13,080  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 28:  
   13,081 .. 13,095  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 29:  GCN4_v4  
   13,096 .. 13,152  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 30:  
   13,153 .. 13,167  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 31:  GCN4_v4  
   13,168 .. 13,224  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 32:  
   13,225 .. 13,239  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 33:  GCN4_v4  
   13,240 .. 13,296  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 34:  
   13,297 .. 13,311  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 35:  GCN4_v4  
   13,312 .. 13,368  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 36:  
   13,369 .. 13,383  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 37:  GCN4_v4  
   13,384 .. 13,440  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 38:  
   13,441 .. 13,455  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 39:  GCN4_v4  
   13,456 .. 13,512  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 40:  
   13,513 .. 13,527  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 41:  GCN4_v4  
   13,528 .. 13,584  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 42:  
   13,585 .. 13,599  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 43:  GCN4_v4  
   13,600 .. 13,656  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 44:  
   13,657 .. 13,671  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 45:  GCN4_v4  
   13,672 .. 13,728  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 46:  
   13,729 .. 13,743  =  15 bp
   5 amino acids  =  363.3 Da
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
   Segment 47:  GCN4_v4  
   13,744 .. 13,800  =  57 bp
   19 amino acids  =  2.3 kDa
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
24xGCN4_v4
12,088 .. 13,800  =  1713 bp
571 amino acids  =  63.1 kDa
47 segments
Product: 24 tandem copies of the GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)
SV40 NLS
13,840 .. 13,860  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
13,840 .. 13,860  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
P2A
13,870 .. 13,926  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1 polyprotein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
P2A
13,870 .. 13,926  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1 polyprotein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
TagBFP
13,939 .. 14,640  =  702 bp
233 amino acids  =  26.3 kDa
Product: monomeric blue fluorescent protein
mammalian codon-optimized
TagBFP
13,939 .. 14,640  =  702 bp
233 amino acids  =  26.3 kDa
Product: monomeric blue fluorescent protein
mammalian codon-optimized
AmpR
1014 .. 1874  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1014 .. 1082  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1014 .. 1874  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1083 .. 1874  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1014 .. 1874  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
LTR
5285 .. 5918  =  634 bp
long terminal repeat (LTR) from HIV-1
LTR
5285 .. 5918  =  634 bp
long terminal repeat (LTR) from HIV-1
ori
2045 .. 2633  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2045 .. 2633  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
SV40 promoter
2879 .. 3208  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
2879 .. 3208  =  330 bp
SV40 enhancer and early promoter
RRE
6582 .. 6815  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
6582 .. 6815  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
LTR (ΔU3)
14,755 .. 77  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
LTR (ΔU3)
14,755 .. 77  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
SV40 promoter
7509 .. 7719  =  211 bp
SV40 early promoter
SV40 promoter
7509 .. 7719  =  211 bp
SV40 early promoter
SV40 poly(A) signal
4982 .. 5116  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4982 .. 5116  =  135 bp
SV40 polyadenylation signal
HIV-1 Ψ
5965 .. 6090  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
5965 .. 6090  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
7329 .. 7446  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
7329 .. 7446  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
909 .. 1013  =  105 bp
AmpR promoter
909 .. 1013  =  105 bp
SV40 ori
3059 .. 3194  =  136 bp
SV40 origin of replication
SV40 ori
3059 .. 3194  =  136 bp
SV40 origin of replication
SV40 ori
7578 .. 7713  =  136 bp
SV40 origin of replication
SV40 ori
7578 .. 7713  =  136 bp
SV40 origin of replication
ORF:  310 .. 672  =  363 bp
ORF:  120 amino acids  =  12.4 kDa
ORF:  4486 .. 4959  =  474 bp
ORF:  157 amino acids  =  18.2 kDa
ORF:  6460 .. 7557  =  1098 bp
ORF:  365 amino acids  =  42.1 kDa
ORF:  7732 .. 14,640  =  6909 bp
ORF:  2302 amino acids  =  260.6 kDa
ORF:  3491 .. 3865  =  375 bp
ORF:  124 amino acids  =  14.0 kDa
ORF:  1014 .. 1874  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3645 .. 3926  =  282 bp
ORF:  93 amino acids  =  10.7 kDa
ORF:  3957 .. 4340  =  384 bp
ORF:  127 amino acids  =  14.9 kDa
ORF:  1478 .. 1744  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3539 .. 3958  =  420 bp
ORF:  139 amino acids  =  15.9 kDa
ORF:  226 .. 537  =  312 bp
ORF:  103 amino acids  =  10.7 kDa
ORF:  7774 .. 12,114  =  4341 bp
ORF:  1446 amino acids  =  152.5 kDa
ORF:  13,720 .. 14,445  =  726 bp
ORF:  241 amino acids  =  25.5 kDa
ORF:  6441 .. 6755  =  315 bp
ORF:  104 amino acids  =  11.0 kDa
ORF:  7713 .. 7937  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  9159 .. 9452  =  294 bp
ORF:  97 amino acids  =  10.7 kDa
ORF:  9723 .. 10,172  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
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Individual Sequences & Maps

3xFLAG-dCas9 pCMV-7.1
pCas9_GFP
pCRISPRa-Enhancer
pME-Cas9-T2A-GFP
3xFLAG-dCas9 pMXs-neo
pCas9D10A_GFP
pCS2+hSpCas9
pMJ806
3xFLAG-dCas9 pMXs-puro
pCas-Guide
pCS2-Cas9
pMJ841
Cas9m4
pcDNA-dCas9
pCS2-nCas9n
pMJ920
Cas9m4-VP64
pcDNA-dCas9-p300 Core
pCW-Cas9
pMLM3705
CRISPRi library backbone
pcDNA-dCas9-p300 Core (D1399Y)
pdCas9
pMSCV-LTR-dCas9-p65AD-BFP
dCAS9-VP64_GFP
pcDNA-dCas9-VP64
pdCas9-bacteria
pMSCV-LTR-dCas9-VP64-BFP
eSpCas9(1.1)
pcDNA3.1-CibN-dCas9-CibN
pdCas9-humanized
pMZ374
GeneArt CRISPR Nuclease Vector - CD4 (linearized)
pCFD1-dU6-1gRNA
pdCas9 BFP-humanized
pNW3
GeneArt CRISPR Nuclease Vector - OFP (linearized)
pCFD2-dU6-2gRNA
pDCC6
pRGE31
gRNA_AAVS1-T1
pCFD3-dU6-3gRNA
pDD162 (Peft-3_Cas9 + Empty sgRNA)
pRGEB31
gRNA_AAVS1-T2
pCFD4-U6-1_U6-3tandemgRNAs
pDR274
pRGEB32
gRNA_Cloning Vector
pCLIP-All-EFS-Ametrine
Peft-3_cas9-SV40_NLS_tbb-2 3'UTR
pROSA26-Puro-DNR
gRNA_GFP-T1
pCLIP-All-EFS-Blast
pET-28b-Cas9-His
pRPR1_gRNA_handle_RPR1t
gRNA_GFP-T2
pCLIP-All-EFS-Puro
pET-Cas9-6xHis
pSECC
HBT-pcoCas9
pCLIP-All-EFS-tRFP
pET-Cas9-NLS-6xHis
pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)
hCas9
pCLIP-All-EFS-ZsGreen
pET-NLS-Cas9-6xHis
pSLQ1658-dCas9-EGFP
hCas9_D10A
pCLIP-All-hCMV-Blast
pET28a Cas9-Cys
pSpCas9(BB)-2A-GFP (PX458)
JDS246
pCLIP-All-hCMV-Puro
pFGC-pcoCas9
pSpCas9(BB)-2A-Puro (PX459) V2.0
lenti dCAS-VP64_Blast
pCLIP-All-hCMV-tRFP
pGL3-U6-sgRNA-PGK-puromycin
pSpCas9n(BB)-2A-GFP (PX461)
lenti MS2-P65-HSF1_Hygro
pCLIP-All-hCMV-ZsGreen
pgRNA-bacteria
pSpCas9n(BB)-2A-Puro (PX462) V2.0
lenti sgRNA(MS2)_zeo backbone
pCLIP-All-Nickase(D10A)-EFS-Blast
pgRNA-humanized
pSpCas9n(BB) (PX460)
lentiCas9-Blast
pCLIP-All-Nickase(D10A)-EFS-Puro
pGuide
pSpCas9 (PX165)
lentiCas9-EGFP
pCLIP-All-Nickase(D10A)-EFS-tRFP
pGuide-it-sgRNA1 Vector (Linear)
pSPgRNA
lentiCas9n(D10A)-Blast
pCLIP-All-Nickase(D10A)-EFS-ZsGreen
pGuide-it-tdTomato Vector (Linear)
pSQT834
lentiCRISPR v2
pCLIP-All-Nickase(D10A)-hCMV-Puro
pGuide-it-ZsGreen1 Vector (Linear)
pSQT1313
lentiGuide-Puro
pCLIP-All-Nickase(D10A)-hCMV-tRFP
pHAGE EF1-alpha dCas9-KRAB
pST1374-N-NLS-flag-linker-Cas9-D10A
LSL-Cas9-Rosa26TV
pCLIP-All-Nickase(D10A)-hCMV-ZsGreen
pHAGE EF1-alpha dCas9-VP64
pST1374-N-NLS-flag-linker-Cas9-H840A
M-SPcas
pCLIP-All-Nickase(D10A)-TRE3G-Blast
pHAGE TRE dCas9
pST1374-NLS-flag-linker-Cas9
M-ST1cas
pCLIP-All-Nickase(D10A)-TRE3G-Puro
pHAGE TRE dCas9-KRAB
pT3TS-nCas9n
MLM3613
pCLIP-All-Nickase(D10A)-TRE3G-tRFP
pHAGE TRE dCas9-VP64
pT7-Cas9
MLM3636
pCLIP-All-Nickase(D10A)-TRE3G-ZsGreen
pHAGE-TO-dCas9-3XGFP
pT7-Cas9-Nickase
MLM3639
pCLIP-All-TRE3G-Blast
pHAGE-TO-dCas9-3XmCherry
pT7-gRNA
MS2-P65-HSF1_GFP
pCLIP-All-TRE3G-Puro
pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2
pT7-Guide-IVT
MSP1101
pCLIP-All-TRE3G-tRFP
pHAGE-TO-nmdCas9-3XGFP
pTDH3-dCas9
MSP2133
pCLIP-All-TRE3G-ZsGreen
pHAGE-TO-nmdCas9-3XmCherry
pTDH3-dCas9-Mxi1
MSP2135
pCLIP-Cas9(D10a)-Nickase-EFS-Puro
pHR-SFFV-dCas9-BFP
pTOL-hCMV-TET3G-Hygro Transactivator
MSP469
pCLIP-Cas9(D10a)-Nickase-EFS-Blast
pHR-SFFV-dCas9-BFP-KRAB
pTPGI_dCas9_VP64
p3s-Cas9HC
pCLIP-Cas9(D10a)-Nickase-EFS-tRFP
pHR-SFFV-KRAB-dCas9-P2A-mCherry
pU6-BbsI-chiRNA
p414-TEF1p-Cas9-CYC1t
pCLIP-Cas9(D10a)-Nickase-EFS-ZsGreen
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
PU6_klp-12_sgRNA
p415-GalL-Cas9-CYC1t
pCLIP-Cas9(D10a)-Nickase-hCMV-Blast
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
p426-SNR52p-gRNA.CAN1.Y-SUP4t
pCLIP-Cas9(D10a)-Nickase-hCMV-Puro
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6
pAAV-Guide-it-1 Vector (Linear)
pCLIP-Cas9(D10a)-Nickase-hCMV-tRFP
pHSN401
pUC119-gRNA
pAAV-Guide-it-Down Vector (Linear)
pCLIP-Cas9(D10a)-Nickase-hCMV-ZsGreen
pHsp70-Cas9
pUC57-sgRNA expression vector
pAAV-Guide-it-Up Vector
pCLIP-Cas9(D10a)-Nickase-TRE3G-Blast
pICH41308_hCas9
Puro-Cas9 donor
pAAVS1-BSD-DNR
pCLIP-Cas9(D10a)-Nickase-TRE3G-Puro
pICH47742_2x35S-5'UTR-hCas9(STOP)-NOST
pWJ66
pAAVS1-Puro-DNR
pCLIP-Cas9(D10a)-Nickase-TRE3G-tRFP
piCRg Entry
pWJ68
pAC2-dual-dCas9VP48-sgExpression
pCLIP-Cas9(D10a)-Nickase-TRE3G-ZsGreen
pICSL01009_AtU6p
pwtCas9-bacteria
pAc-sgRNA-Cas9
pCLIP-Cas9-Nuclease-EFS-Blast
pK7WGF2_hCas9
pX260
pAC149-pCR8-dCas9VP160
pCLIP-Cas9-Nuclease-EFS-Puro
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
pX330
pAC152-dual-dCas9VP64-sgExpression
pCLIP-Cas9-Nuclease-EFS-tRFP
pL-CRISPR.EFS.GFP
pX330A-1x2
pAC153-dual-dCas9VP96-sgExpression
pCLIP-Cas9-Nuclease-EFS-ZsGreen
pL-CRISPR.EFS.tRFP
pX330S-2
pAC154-dual-dCas9VP160-sgExpression
pCLIP-Cas9-Nuclease-hCMV-Blast
pL-CRISPR.SFFV.GFP
pX334
pAC155-pCR8-sgExpression
pCLIP-Cas9-Nuclease-hCMV-Puro
pL-CRISPR.SFFV.tRFP
pX335
pAC84-pCR8-dCas9
pCLIP-Cas9-Nuclease-hCMV-tRFP
pLenti-Cas9
PX551
pAC91-pmax-dCas9VP64
pCLIP-Cas9-Nuclease-hCMV-ZsGreen
pLenti-Cas9-IRES-Puro
PX552
pAC93-pmax-dCas9VP160
pCLIP-Cas9-Nuclease-TRE3G-Blast
pLenti-Cas9-P2A-tGFP
pX600
pAC94-pmax-dCas9VP160-2A-puro
pCLIP-Cas9-Nuclease-TRE3G-Puro
pLenti-Cas-Guide
pX601
pAC95-pmax-dCas9VP160-2A-neo
pCLIP-Cas9-Nuclease-TRE3G-tRFP
pLenti-EF1a-Cas9-IRES-Puro
pX602
pACT2-CAS9c
pCLIP-Cas9-Nuclease-TRE3G-ZsGreen
pLenti-Guide-Puro
PX851
pBS-Hsp70-Cas9
pCLIP-gRNA-EFS-Blast
pLKO.1-puro U6 sgRNA BfuAI stuffer
PX852
pCAG-T3-hCAS-pA
pCLIP-gRNA-EFS-Puro
pLSC-5
PX853
pCAG-T3-hCasD10A-pA
pCLIP-gRNA-EFS-tRFP
pLV hUbC-Cas9-T2A-GFP
PX854
pCas
pCLIP-gRNA-EFS-ZsGreen
pLV hUbC-dCas9 VP64-T2A-GFP
PX855
pCas-Guide-AAVS1
pCLIP-gRNA-hCMV-Blast
pLV hUbC-dCas9-T2A-GFP
PX856
pCas-Guide-CRISPRa
pCLIP-gRNA-hCMV-Puro
pLVX-hyg-sgRNA1 Vector (Linear)
pY010 (pcDNA3.1-hAsCpf1)
pCas-Guide-CRISPRi
pCLIP-gRNA-hCMV-tRFP
pLVX-puro-Cas9
pY016 (pcDNA3.1-hLbCpf1)
pCas-Guide-EF1a-CD4
pCLIP-gRNA-hCMV-ZsGreen
pLX-sgRNA
sgRNA(MS2) cloning backbone
pCas-Guide-EF1a-GFP
pCRCT
pMB66
SP-Cas9
pCas-Guide-Nickase
pCRISPomyces-1
pMB70
SP-dCas9-VPR
pCas-Guide-ROSA26
pCRISPomyces-2
pME-Cas9
VP12
pCas9
pCRISPR

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

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