pCS2+hSpCas9

Plasmid for in vitro transcription of human codon-optimized S. pyogenes Cas9 using the SP6 promoter.

Sequence Author: Kinoshita Lab / Addgene #51815

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ATG SV40 NLS pCS2+hSpCas9 8343 bp
ATG
91 .. 93  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
91 .. 93  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
3xFLAG
94 .. 159  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
3xFLAG
94 .. 159  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
SV40 NLS
166 .. 186  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
166 .. 186  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Cas9
211 .. 4311  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
211 .. 4311  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
nucleoplasmin NLS
4312 .. 4359  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
nucleoplasmin NLS
4312 .. 4359  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
CMV IE94 promoter
7359 .. 8343  =  985 bp
enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94
CMV IE94 promoter
7359 .. 8343  =  985 bp
enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94
AmpR
5839 .. 6699  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5839 .. 6630  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5839 .. 6699  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6631 .. 6699  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5839 .. 6699  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
5080 .. 5668  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5080 .. 5668  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
6830 .. 7285  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
6830 .. 7285  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 poly(A) signal
4392 .. 4526  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4392 .. 4526  =  135 bp
SV40 polyadenylation signal
AmpR promoter
6700 .. 6804  =  105 bp
AmpR promoter
6700 .. 6804  =  105 bp
SP6 promoter
35 .. 53  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
35 .. 53  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
ORF:  91 .. 4362  =  4272 bp
ORF:  1423 amino acids  =  164.6 kDa
ORF:  5969 .. 6235  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  7394 .. 7792  =  399 bp
ORF:  132 amino acids  =  14.3 kDa
ORF:  7377 .. 7625  =  249 bp
ORF:  82 amino acids  =  9.1 kDa
ORF:  8025 .. 8297  =  273 bp
ORF:  90 amino acids  =  9.5 kDa
ORF:  61 .. 2727  =  2667 bp
ORF:  888 amino acids  =  93.5 kDa
ORF:  5839 .. 6699  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1557 .. 1850  =  294 bp
ORF:  97 amino acids  =  10.8 kDa
ORF:  2121 .. 2570  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
ORF:  7068 .. 7364  =  297 bp
ORF:  98 amino acids  =  10.6 kDa
ORF:  7298 .. 7654  =  357 bp
ORF:  118 amino acids  =  13.0 kDa
ORF:  7997 .. 8278  =  282 bp
ORF:  93 amino acids  =  10.1 kDa
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Download pCS2+hSpCas9.dna file

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