pET-28b-Cas9-His

Bacterial plasmid for expressing a 6xHis-tagged Cas9 protein, which can be purified and microinjected for gene editing.

Sequence Author: Schier Lab / Addgene #47327

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T7 promoter RBS pET-28b-Cas9-His 9375 bp
Cas9
2802 .. 6905  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
2802 .. 6905  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
6918 .. 6938  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
6918 .. 6938  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
6xHis
6954 .. 6971  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
6954 .. 6971  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
lacI
1246 .. 2328  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
1246 .. 2328  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
KanR
7669 .. 8484  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
KanR
7669 .. 8484  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
ori
8606 .. 9194  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
8606 .. 9194  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
7122 .. 7577  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
7122 .. 7577  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
rop
246 .. 437  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein, which maintains plasmids at low copy number
rop
246 .. 437  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein, which maintains plasmids at low copy number
bom
5 .. 144  =  140 bp
basis of mobility region from pBR322
bom
5 .. 144  =  140 bp
basis of mobility region from pBR322
lacI promoter
2329 .. 2406  =  78 bp
lacI promoter
2329 .. 2406  =  78 bp
T7 terminator
7038 .. 7085  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
7038 .. 7085  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
lac operator
2734 .. 2758  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
2734 .. 2758  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RBS
2773 .. 2795  =  23 bp
efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
RBS
2773 .. 2795  =  23 bp
efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
T7 promoter
2715 .. 2733  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2715 .. 2733  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  469 .. 837  =  369 bp
ORF:  122 amino acids  =  14.2 kDa
ORF:  2504 .. 2743  =  240 bp
ORF:  79 amino acids  =  8.0 kDa
ORF:  1212 .. 1463  =  252 bp
ORF:  83 amino acids  =  9.1 kDa
ORF:  2367 .. 2630  =  264 bp
ORF:  87 amino acids  =  9.5 kDa
ORF:  2802 .. 6974  =  4173 bp
ORF:  1390 amino acids  =  161.0 kDa
ORF:  1246 .. 2205  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
ORF:  7669 .. 8484  =  816 bp
ORF:  271 amino acids  =  31.0 kDa
ORF:  246 .. 470  =  225 bp
ORF:  74 amino acids  =  8.5 kDa
ORF:  834 .. 1190  =  357 bp
ORF:  118 amino acids  =  13.0 kDa
ORF:  2784 .. 3281  =  498 bp
ORF:  165 amino acids  =  18.3 kDa
ORF:  4206 .. 4724  =  519 bp
ORF:  172 amino acids  =  20.1 kDa
ORF:  4788 .. 5321  =  534 bp
ORF:  177 amino acids  =  19.9 kDa
ORF:  6315 .. 6848  =  534 bp
ORF:  177 amino acids  =  20.0 kDa
ORF:  1226 .. 1489  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  2078 .. 2578  =  501 bp
ORF:  166 amino acids  =  17.5 kDa
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Download pET-28b-Cas9-His.dna file

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