MLM3636

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No matches
BsmBI (325) BsmBI (350) MLM3636 2278 bp
BsmBI  (325)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
BsmBI  (350)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
AmpR
1288 .. 2148  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1288 .. 2079  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1288 .. 2148  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2080 .. 2148  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1288 .. 2148  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
529 .. 1117  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
529 .. 1117  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
U6 promoter
82 .. 330  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
82 .. 330  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
AmpR promoter
2149 .. 2253  =  105 bp
AmpR promoter
2149 .. 2253  =  105 bp
gRNA scaffold
350 .. 425  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
350 .. 425  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
polIII terminator
426 .. 431  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
426 .. 431  =  6 bp
RNA polymerase III transcription terminator
ORF:  1418 .. 1684  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  465 .. 854  =  390 bp
ORF:  129 amino acids  =  14.4 kDa
ORF:  1288 .. 2148  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Download MLM3636.dna file

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Individual Sequences & Maps

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