p3s-Cas9HC

Mammalian cell vector for expressing S. pyogenes Cas9 with a C-terminal HA epitope tag.

Sequence Author: Kim Lab / Addgene #43945

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SV40 NLS p3s-Cas9HC 7383 bp
Cas9
901 .. 5004  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
901 .. 5004  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
5020 .. 5040  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5020 .. 5040  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
HA
5041 .. 5067  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
5041 .. 5067  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
AmpR
6387 .. 7247  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6387 .. 7178  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6387 .. 7247  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   7179 .. 7247  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6387 .. 7247  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
5628 .. 6216  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5628 .. 6216  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
bGH poly(A) signal
5145 .. 5369  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
5145 .. 5369  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
7248 .. 7352  =  105 bp
AmpR promoter
7248 .. 7352  =  105 bp
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  901 .. 5070  =  4170 bp
ORF:  1389 amino acids  =  160.7 kDa
ORF:  6517 .. 6783  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  774 .. 5090  =  4317 bp
ORF:  1438 amino acids  =  161.3 kDa
ORF:  862 .. 5082  =  4221 bp
ORF:  1406 amino acids  =  141.8 kDa
ORF:  2250 .. 2543  =  294 bp
ORF:  97 amino acids  =  10.9 kDa
ORF:  2865 .. 3263  =  399 bp
ORF:  132 amino acids  =  15.6 kDa
ORF:  6387 .. 7247  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Download p3s-Cas9HC.dna file

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