pTOL-hCMV-TET3G-Hygro Transactivator

Lentiviral vector with a hygromycin resistance marker for expression of the Tet-On® 3G doxycycline-dependent transactivator.

Sequence Author: transOMIC

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pTOL-hCMV-TET3G-Hygro Transactivator 11,272 bp
HygR
4528 .. 5553  =  1026 bp
341 amino acids  =  38.0 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
HygR
4528 .. 5553  =  1026 bp
341 amino acids  =  38.0 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
HygR
7793 .. 8818  =  1026 bp
341 amino acids  =  38.0 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
HygR
7793 .. 8818  =  1026 bp
341 amino acids  =  38.0 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
AmpR
10,276 .. 11,136  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   10,276 .. 11,067  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
10,276 .. 11,136  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   11,068 .. 11,136  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
10,276 .. 11,136  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
Tet-On® 3G
3188 .. 3934  =  747 bp
248 amino acids  =  27.7 kDa
Product: modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline
Tet-On® 3G
3188 .. 3934  =  747 bp
248 amino acids  =  27.7 kDa
Product: modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline
LTR
367 .. 1000  =  634 bp
long terminal repeat (LTR) from HIV-1
LTR
367 .. 1000  =  634 bp
long terminal repeat (LTR) from HIV-1
WPRE
5567 .. 6155  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
5567 .. 6155  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
9520 .. 10,105  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
9520 .. 10,105  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
IRES
3976 .. 4525  =  550 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
IRES
3976 .. 4525  =  550 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
f1 ori
6973 .. 7401  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
6973 .. 7401  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
7415 .. 7744  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
7415 .. 7744  =  330 bp
SV40 enhancer and early promoter
CMV enhancer
2567 .. 2870  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2567 .. 2870  =  304 bp
human cytomegalovirus immediate early enhancer
RRE
1669 .. 1902  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1669 .. 1902  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
LTR (ΔU3)
6363 .. 6596  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
LTR (ΔU3)
6363 .. 6596  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
bGH poly(A) signal
6703 .. 6927  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
6703 .. 6927  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
2871 .. 3074  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2871 .. 3074  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
HIV-1 Ψ
1047 .. 1172  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
1047 .. 1172  =  126 bp
packaging signal of human immunodeficiency virus type 1
SV40 poly(A) signal
8948 .. 9069  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
8948 .. 9069  =  122 bp
SV40 polyadenylation signal
cPPT/CTS
2394 .. 2509  =  116 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
cPPT/CTS
2394 .. 2509  =  116 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
AmpR promoter
11,137 .. 11,241  =  105 bp
AmpR promoter
11,137 .. 11,241  =  105 bp
SV40 ori
7595 .. 7730  =  136 bp
SV40 origin of replication
SV40 ori
7595 .. 7730  =  136 bp
SV40 origin of replication
ORF:  4528 .. 5553  =  1026 bp
ORF:  341 amino acids  =  38.0 kDa
ORF:  1547 .. 2398  =  852 bp
ORF:  283 amino acids  =  32.7 kDa
ORF:  3188 .. 3934  =  747 bp
ORF:  248 amino acids  =  27.7 kDa
ORF:  5657 .. 6184  =  528 bp
ORF:  175 amino acids  =  19.0 kDa
ORF:  7793 .. 8818  =  1026 bp
ORF:  341 amino acids  =  38.0 kDa
ORF:  10,406 .. 10,672  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3345 .. 3647  =  303 bp
ORF:  100 amino acids  =  11.1 kDa
ORF:  5670 .. 5966  =  297 bp
ORF:  98 amino acids  =  10.2 kDa
ORF:  6924 .. 7190  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  7730 .. 8551  =  822 bp
ORF:  273 amino acids  =  30.8 kDa
ORF:  1381 .. 1842  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
ORF:  3640 .. 3921  =  282 bp
ORF:  93 amino acids  =  9.6 kDa
ORF:  4441 .. 5286  =  846 bp
ORF:  281 amino acids  =  31.5 kDa
ORF:  10,276 .. 11,136  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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