M-ST1cas

Mammalian cell vector for expressing human codon-optimized Streptococcus thermophilus Cas9 with a G418-resistance marker.

Sequence Author: Church Lab / Addgene #48669

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M-ST1cas 8813 bp
St1Cas9
748 .. 4110  =  3363 bp
1121 amino acids  =  129.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus thermophilus Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
St1Cas9
748 .. 4110  =  3363 bp
1121 amino acids  =  129.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus thermophilus Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
4123 .. 4143  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4123 .. 4143  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
AmpR
7632 .. 8492  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   7632 .. 8423  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7632 .. 8492  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   8424 .. 8492  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7632 .. 8492  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
5331 .. 6125  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
5331 .. 6125  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
6873 .. 7461  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6873 .. 7461  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
4493 .. 4921  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4493 .. 4921  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
50 .. 429  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
50 .. 429  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
4935 .. 5264  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
4935 .. 5264  =  330 bp
SV40 enhancer and early promoter
CMV promoter
430 .. 633  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
430 .. 633  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
6301 .. 6422  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
6301 .. 6422  =  122 bp
SV40 polyadenylation signal
AmpR promoter
8493 .. 8597  =  105 bp
AmpR promoter
8493 .. 8597  =  105 bp
HSV TK poly(A) signal
4243 .. 4291  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
4243 .. 4291  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
SV40 ori
5115 .. 5250  =  136 bp
SV40 origin of replication
SV40 ori
5115 .. 5250  =  136 bp
SV40 origin of replication
ORF:  589 .. 4146  =  3558 bp
ORF:  1185 amino acids  =  137.3 kDa
ORF:  5503 .. 5889  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  7762 .. 8028  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5331 .. 6125  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  1875 .. 2150  =  276 bp
ORF:  91 amino acids  =  10.2 kDa
ORF:  2178 .. 2561  =  384 bp
ORF:  127 amino acids  =  14.7 kDa
ORF:  5640 .. 5894  =  255 bp
ORF:  84 amino acids  =  9.6 kDa
ORF:  7632 .. 8492  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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