pAd PL-DEST

Promoterless adenoviral Gateway® destination vector for inserting a gene expression cassette.

Sequence Author: Thermo Fisher (Invitrogen)

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Explore Over 2.7k Plasmids: Gateway® Cloning Vectors | More Plasmid Sets
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HomePlasmidsGateway® Cloning VectorspAd PL-DEST
5' ITR BstBI (29,683) SpeI (25,803) MreI (25,110) AsiSI (20,163) attR1 lac UV5 promoter attR2 PmeI (11,979) pAd/PL-DEST™ 34,864 bp
BstBI  (29,683)
1 site		 T T C G A A A A G C T T
SpeI  (25,803)
1 site		 A C T A G T T G A T C A
MreI  (25,110)
1 site		 C G C C G G C G G C G G C C G C
AsiSI  (20,163)
1 site		 G C G A T C G C C G C T A G C G
PmeI  (11,979)
1 site		 G T T T A A A C C A A A T T T G
adenoviral DNA
2234 .. 32,782  =  30,549 bp
sequences from human adenovirus serotype 5, lacking the E1 and E3 regions
adenoviral DNA
2234 .. 32,782  =  30,549 bp
sequences from human adenovirus serotype 5, lacking the E1 and E3 regions
AmpR
33,746 .. 34,606  =  861 bp
286 amino acids  =  ~31.6 kDa
2 segments
   Segment 2:  
   33,746 .. 34,537  =  792 bp
   263 amino acids  =  ~29.0 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
33,746 .. 34,606  =  861 bp
286 amino acids  =  ~31.6 kDa
2 segments
   Segment 1:  signal sequence  
   34,538 .. 34,606  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
33,746 .. 34,606  =  861 bp
286 amino acids  =  ~31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
CmR
745 .. 1404  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
745 .. 1404  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
33,006 .. 33,594  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
33,006 .. 33,594  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ccdB
1746 .. 2051  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
ccdB
1746 .. 2051  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
Ad5 Ψ
191 .. 341  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
191 .. 341  =  151 bp
packaging signal for adenovirus serotype 5
attR1
512 .. 636  =  125 bp
recombination site for the Gateway® LR reaction
attR1
512 .. 636  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2092 .. 2216  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2092 .. 2216  =  125 bp
recombination site for the Gateway® LR reaction
AmpR promoter
34,607 .. 34,711  =  105 bp
AmpR promoter
34,607 .. 34,711  =  105 bp
5' ITR
1 .. 103  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
5' ITR
1 .. 103  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
lac UV5 promoter
661 .. 691  =  31 bp
3 segments
   Segment 1:  -35  
   661 .. 666  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
661 .. 691  =  31 bp
3 segments
   Segment 2:  
   667 .. 684  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
661 .. 691  =  31 bp
3 segments
   Segment 3:  -10  
   685 .. 691  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
661 .. 691  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
3' ITR
32,680 .. 32,782  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
3' ITR
32,680 .. 32,782  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ORF:  745 .. 1404  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  5011 .. 5331  =  321 bp
ORF:  106 amino acids  =  11.7 kDa
ORF:  6031 .. 6282  =  252 bp
ORF:  83 amino acids  =  9.4 kDa
ORF:  7216 .. 7452  =  237 bp
ORF:  78 amino acids  =  7.9 kDa
ORF:  12,877 .. 14,592  =  1716 bp
ORF:  571 amino acids  =  63.3 kDa
ORF:  14,599 .. 15,195  =  597 bp
ORF:  198 amino acids  =  22.0 kDa
ORF:  15,232 .. 16,371  =  1140 bp
ORF:  379 amino acids  =  42.6 kDa
ORF:  16,399 .. 16,641  =  243 bp
ORF:  80 amino acids  =  8.8 kDa
ORF:  17,563 .. 20,421  =  2859 bp
ORF:  952 amino acids  =  108.0 kDa
ORF:  22,456 .. 22,812  =  357 bp
ORF:  118 amino acids  =  12.8 kDa
ORF:  23,155 .. 23,541  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  24,718 .. 25,506  =  789 bp
ORF:  262 amino acids  =  28.9 kDa
ORF:  29,578 .. 29,976  =  399 bp
ORF:  132 amino acids  =  15.0 kDa
ORF:  30,799 .. 31,296  =  498 bp
ORF:  165 amino acids  =  18.7 kDa
ORF:  32,851 .. 33,087  =  237 bp
ORF:  78 amino acids  =  8.9 kDa
ORF:  2330 .. 2752  =  423 bp
ORF:  140 amino acids  =  14.5 kDa
ORF:  9152 .. 9565  =  414 bp
ORF:  137 amino acids  =  14.4 kDa
ORF:  11,039 .. 12,796  =  1758 bp
ORF:  585 amino acids  =  65.3 kDa
ORF:  16,007 .. 16,486  =  480 bp
ORF:  159 amino acids  =  17.4 kDa
ORF:  16,724 .. 17,476  =  753 bp
ORF:  250 amino acids  =  27.0 kDa
ORF:  25,895 .. 26,578  =  684 bp
ORF:  227 amino acids  =  24.7 kDa
ORF:  26,579 .. 26,902  =  324 bp
ORF:  107 amino acids  =  12.3 kDa
ORF:  27,320 .. 27,682  =  363 bp
ORF:  120 amino acids  =  13.7 kDa
ORF:  31,310 .. 31,648  =  339 bp
ORF:  112 amino acids  =  12.5 kDa
ORF:  32,933 .. 33,331  =  399 bp
ORF:  132 amino acids  =  14.8 kDa
ORF:  1746 .. 2051  =  306 bp
ORF:  101 amino acids  =  11.7 kDa
ORF:  6699 .. 7148  =  450 bp
ORF:  149 amino acids  =  16.7 kDa
ORF:  8025 .. 8531  =  507 bp
ORF:  168 amino acids  =  17.7 kDa
ORF:  9771 .. 11,018  =  1248 bp
ORF:  415 amino acids  =  47.1 kDa
ORF:  20,454 .. 21,068  =  615 bp
ORF:  204 amino acids  =  23.1 kDa
ORF:  22,200 .. 25,205  =  3006 bp
ORF:  1001 amino acids  =  110.0 kDa
ORF:  27,828 .. 29,630  =  1803 bp
ORF:  600 amino acids  =  63.6 kDa
ORF:  33,876 .. 34,142  =  267 bp
ORF:  88 amino acids  =  ~9.3 kDa
ORF:  4058 .. 4405  =  348 bp
ORF:  115 amino acids  =  12.2 kDa
ORF:  7304 .. 9265  =  1962 bp
ORF:  653 amino acids  =  74.7 kDa
ORF:  10,280 .. 10,573  =  294 bp
ORF:  97 amino acids  =  10.5 kDa
ORF:  17,186 .. 17,515  =  330 bp
ORF:  109 amino acids  =  11.4 kDa
ORF:  21,164 .. 22,753  =  1590 bp
ORF:  529 amino acids  =  59.1 kDa
ORF:  25,148 .. 25,438  =  291 bp
ORF:  96 amino acids  =  10.5 kDa
ORF:  28,001 .. 28,471  =  471 bp
ORF:  156 amino acids  =  16.6 kDa
ORF:  28,805 .. 29,050  =  246 bp
ORF:  81 amino acids  =  8.7 kDa
ORF:  31,196 .. 31,546  =  351 bp
ORF:  116 amino acids  =  13.3 kDa
ORF:  33,746 .. 34,606  =  861 bp
ORF:  286 amino acids  =  ~31.6 kDa
ORF:  2812 .. 4170  =  1359 bp
ORF:  452 amino acids  =  51.2 kDa
ORF:  5173 .. 5511  =  339 bp
ORF:  112 amino acids  =  12.8 kDa
ORF:  7183 .. 7761  =  579 bp
ORF:  192 amino acids  =  20.8 kDa
ORF:  9475 .. 9840  =  366 bp
ORF:  121 amino acids  =  12.7 kDa
ORF:  11,035 .. 11,295  =  261 bp
ORF:  86 amino acids  =  9.0 kDa
ORF:  13,102 .. 13,887  =  786 bp
ORF:  261 amino acids  =  28.5 kDa
ORF:  15,364 .. 15,633  =  270 bp
ORF:  89 amino acids  =  9.5 kDa
ORF:  16,882 .. 17,430  =  549 bp
ORF:  182 amino acids  =  18.9 kDa
ORF:  30,841 .. 31,185  =  345 bp
ORF:  114 amino acids  =  13.3 kDa
ORF:  31,984 .. 32,370  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  32,740 .. 33,036  =  297 bp
ORF:  98 amino acids  =  10.8 kDa
ORF:  3195 .. 3419  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  3918 .. 7088  =  3171 bp
ORF:  1056 amino acids  =  120.4 kDa
ORF:  9981 .. 10,349  =  369 bp
ORF:  122 amino acids  =  13.6 kDa
ORF:  15,918 .. 16,169  =  252 bp
ORF:  83 amino acids  =  8.3 kDa
ORF:  16,368 .. 16,658  =  291 bp
ORF:  96 amino acids  =  10.9 kDa
ORF:  17,595 .. 17,870  =  276 bp
ORF:  91 amino acids  =  10.2 kDa
ORF:  30,036 .. 30,920  =  885 bp
ORF:  294 amino acids  =  34.1 kDa
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Download pAd PL-DEST.dna file

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Individual Sequences & Maps

pAd BLOCK-iT-DEST
pDEST R4-R3 Vector II
pENTR11
pHELLSGATE 4
pAd CMV V5-DEST
pDEST10
pENTR1A
pHELLSGATE 8
pAd PL-DEST
pDEST14
pENTR2B
pIB V5-His-DEST
pBAD-DEST49
pDEST15
pENTR3C
pLenti4 BLOCK-iT-DEST
pcDNA-DEST40
pDEST17
pENTR4
pLenti4 V5-DEST
pcDNA-DEST47
pDEST20
pET-53-DEST
pLenti6.2 C-Lumio V5-DEST
pcDNA-DEST53
pDEST24
pET-54-DEST
pLenti6.2 N-Lumio V5-DEST
pcDNA3.1 nV5-DEST
pDEST26
pET-55-DEST
pLenti6.2 V5-DEST
pcDNA3.2 capTEV-CT V5-DEST
pDEST27
pET-56-DEST
pLenti6.3 TO V5-DEST
pcDNA3.2 capTEV-NT V5-DEST
pDEST8
pET-57-DEST
pLenti6.3 V5-DEST
pcDNA3.2 V5-DEST
pDONR221 P5-P2
pET-58-DEST
pLenti6.4 R4R2 V5-DEST
pcDNA6.2 C-EmGFP-DEST
pDONR P2r-P3
pET-59-DEST
pLenti6 BLOCK-iT-DEST
pcDNA6.2 C-YFP-DEST
pDONR P4-P1r
pET-60-DEST
pLenti6 capTEV-CT-DEST
pcDNA6.2 cGeneBLAzer-DEST
pDONR Zeo
pET-61-DEST
pLenti6 capTEV-NT-DEST1
pcDNA6.2 cLumio-DEST
pDONR201
pET-62-DEST
pLenti6 UbC V5-DEST
pcDNA6.2 EmGFP-Bsd V5-DEST
pDONR207
pET-DEST42
pLenti6 V5-DEST
pcDNA6.2 N-EmGFP-DEST
pDONR221
pET104-DEST
pLenti7.3 V5-DEST
pcDNA6.2 N-YFP-DEST
pDONR221 P1-P4
pET104.1-DEST
pMT-DEST48
pcDNA6.2 nGeneBLAzer-DEST
pDONR221 P1-P5r
pET160-DEST
pMT BioEase-DEST
pcDNA6.2 nLumio-DEST
pDONR221 P3-P2
pET161-DEST
pSCREEN-iT lacZ-DEST
pcDNA6.2 V5-DEST
pDONR221 P4r-P3r
pEXP1-DEST
pT-REx-DEST30
pcDNA6.2 V5-pL-DEST
pDONR221 P5-P4
pEXP2-DEST
pT-REx-DEST31
pCMV SPORT6
pDONR222
pEXP3-DEST
pYES-DEST52
pCMV SPORT6.1
pDONR223
pEXP4-DEST
Reading Frame Cassette A
pCOLA-2-DEST
pEF-DEST51
pHELLSGATE
Reading Frame Cassette B
pCOLA-3-DEST
pEF5 FRT V5-DEST
pHELLSGATE 12
Reading Frame Cassette C.1
pDEST R4-R3
pENTR GeneBLAzer

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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