pcDNA-DEST47

Gateway® destination vector for expressing C-terminally GFP-tagged proteins in mammalian cells.

Sequence Author: Thermo Fisher (Invitrogen)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Gateway® Cloning Vectors | More Plasmid Sets
No matches
HomePlasmidsGateway® Cloning VectorspcDNA-DEST47
SgrDI (7778) PvuI (7227) AhdI (6857) PciI (5964) PfoI (5388) RsrII (5127) PflFI - Tth111I (4729) PluTI (4614) SfoI (4612) NarI (4611) KasI (4610) AvrII (4401) StuI (4400) BseRI (4397) CsiI - SexAI * (4168) BglII (12) SpeI (249) CMV enhancer SnaBI (590) NotI (1040) lac UV5 promoter EcoRI (1360) BsmBI - Esp3I (1584) PasI - PflMI * (1592) BbvCI (2084) SrfI (2230) BmgBI (2264) BstXI (2347) NheI (2631) BmtI (2635) PaeR7I - PspXI - XhoI (3051) BstBI (3252) BbsI (3564) DraIII (3878) pcDNA™-DEST47 7780 bp
SgrDI  (7778)
1 site		 C G T C G A C G G C A G C T G C
PvuI  (7227)
1 site		 C G A T C G G C T A G C
AhdI  (6857)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (5964)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
PfoI  (5388)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
RsrII  (5127)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (4729)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4729)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PluTI  (4614)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (4612)
1 site		 G G C G C C C C G C G G
NarI  (4611)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (4610)
1 site		 G G C G C C C C G C G G
AvrII  (4401)
1 site		 C C T A G G G G A T C C
StuI  (4400)
1 site		 A G G C C T T C C G G A
BseRI  (4397)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
CsiI  (4168)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (4168)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BglII  (12)
1 site		 A G A T C T T C T A G A
SpeI  (249)
1 site		 A C T A G T T G A T C A
SnaBI  (590)
1 site		 T A C G T A A T G C A T
NotI  (1040)
1 site		 G C G G C C G C C G C C G G C G
EcoRI  (1360)
1 site		 G A A T T C C T T A A G
BsmBI  (1584)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (1584)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
PasI  (1592)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
PflMI  (1592)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
BbvCI  (2084)
1 site		 C C T C A G C G G A G T C G
SrfI  (2230)
1 site		 G C C C G G G C C G G G C C C G
BmgBI  (2264)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BstXI  (2347)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
NheI  (2631)
1 site		 G C T A G C C G A T C G
BmtI  (2635)
1 site		 G C T A G C C G A T C G
PaeR7I  (3051)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (3051)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (3051)
1 site		 C T C G A G G A G C T C
BstBI  (3252)
1 site		 T T C G A A A A G C T T
BbsI  (3564)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
DraIII  (3878)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
AmpR
6784 .. 7644  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6784 .. 7575  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6784 .. 7644  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   7576 .. 7644  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6784 .. 7644  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
4483 .. 5277  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
4483 .. 5277  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
Cycle 3 GFP
2628 .. 3347  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 1:  
   2628 .. 2630  =  3 bp
   1 amino acid  =  149.2 Da
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
Cycle 3 GFP
2628 .. 3347  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 2:  1a  
   2631 .. 2633  =  3 bp
   1 amino acid  =  89.1 Da
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
Cycle 3 GFP
2628 .. 3347  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 3:  
   2634 .. 3347  =  714 bp
   237 amino acids  =  26.7 kDa
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
Cycle 3 GFP
2628 .. 3347  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
Product: Cycle 3 GFP (Crameri et al., 1996)
mammalian codon-optimized
CmR
1147 .. 1827  =  681 bp
226 amino acids  =  26.6 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
1147 .. 1827  =  681 bp
226 amino acids  =  26.6 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
6025 .. 6613  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6025 .. 6613  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
3645 .. 4073  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
3645 .. 4073  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
235 .. 614  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
4087 .. 4416  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
4087 .. 4416  =  330 bp
SV40 enhancer and early promoter
ccdB
2147 .. 2452  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
ccdB
2147 .. 2452  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
bGH poly(A) signal
3375 .. 3599  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
3375 .. 3599  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
615 .. 818  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
attR1
914 .. 1038  =  125 bp
recombination site for the Gateway® LR reaction
attR1
914 .. 1038  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2493 .. 2617  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2493 .. 2617  =  125 bp
recombination site for the Gateway® LR reaction
SV40 poly(A) signal
5453 .. 5574  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
5453 .. 5574  =  122 bp
SV40 polyadenylation signal
AmpR promoter
7645 .. 7749  =  105 bp
AmpR promoter
7645 .. 7749  =  105 bp
lac UV5 promoter
1063 .. 1093  =  31 bp
3 segments
   Segment 1:  -35  
   1063 .. 1068  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1063 .. 1093  =  31 bp
3 segments
   Segment 2:  
   1069 .. 1086  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1063 .. 1093  =  31 bp
3 segments
   Segment 3:  -10  
   1087 .. 1093  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1063 .. 1093  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
863 .. 881  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
4267 .. 4402  =  136 bp
SV40 origin of replication
SV40 ori
4267 .. 4402  =  136 bp
SV40 origin of replication
ORF:  1147 .. 1827  =  681 bp
ORF:  226 amino acids  =  26.6 kDa
ORF:  4483 .. 5277  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2147 .. 2452  =  306 bp
ORF:  101 amino acids  =  11.7 kDa
ORF:  3596 .. 3862  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  4655 .. 5041  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  6914 .. 7180  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2628 .. 3347  =  720 bp
ORF:  239 amino acids  =  26.9 kDa
ORF:  4792 .. 5046  =  255 bp
ORF:  84 amino acids  =  9.6 kDa
ORF:  6784 .. 7644  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pcDNA-DEST47.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

pAd BLOCK-iT-DEST
pDEST R4-R3 Vector II
pENTR11
pHELLSGATE 4
pAd CMV V5-DEST
pDEST10
pENTR1A
pHELLSGATE 8
pAd PL-DEST
pDEST14
pENTR2B
pIB V5-His-DEST
pBAD-DEST49
pDEST15
pENTR3C
pLenti4 BLOCK-iT-DEST
pcDNA-DEST40
pDEST17
pENTR4
pLenti4 V5-DEST
pcDNA-DEST47
pDEST20
pET-53-DEST
pLenti6.2 C-Lumio V5-DEST
pcDNA-DEST53
pDEST24
pET-54-DEST
pLenti6.2 N-Lumio V5-DEST
pcDNA3.1 nV5-DEST
pDEST26
pET-55-DEST
pLenti6.2 V5-DEST
pcDNA3.2 capTEV-CT V5-DEST
pDEST27
pET-56-DEST
pLenti6.3 TO V5-DEST
pcDNA3.2 capTEV-NT V5-DEST
pDEST8
pET-57-DEST
pLenti6.3 V5-DEST
pcDNA3.2 V5-DEST
pDONR221 P5-P2
pET-58-DEST
pLenti6.4 R4R2 V5-DEST
pcDNA6.2 C-EmGFP-DEST
pDONR P2r-P3
pET-59-DEST
pLenti6 BLOCK-iT-DEST
pcDNA6.2 C-YFP-DEST
pDONR P4-P1r
pET-60-DEST
pLenti6 capTEV-CT-DEST
pcDNA6.2 cGeneBLAzer-DEST
pDONR Zeo
pET-61-DEST
pLenti6 capTEV-NT-DEST1
pcDNA6.2 cLumio-DEST
pDONR201
pET-62-DEST
pLenti6 UbC V5-DEST
pcDNA6.2 EmGFP-Bsd V5-DEST
pDONR207
pET-DEST42
pLenti6 V5-DEST
pcDNA6.2 N-EmGFP-DEST
pDONR221
pET104-DEST
pLenti7.3 V5-DEST
pcDNA6.2 N-YFP-DEST
pDONR221 P1-P4
pET104.1-DEST
pMT-DEST48
pcDNA6.2 nGeneBLAzer-DEST
pDONR221 P1-P5r
pET160-DEST
pMT BioEase-DEST
pcDNA6.2 nLumio-DEST
pDONR221 P3-P2
pET161-DEST
pSCREEN-iT lacZ-DEST
pcDNA6.2 V5-DEST
pDONR221 P4r-P3r
pEXP1-DEST
pT-REx-DEST30
pcDNA6.2 V5-pL-DEST
pDONR221 P5-P4
pEXP2-DEST
pT-REx-DEST31
pCMV SPORT6
pDONR222
pEXP3-DEST
pYES-DEST52
pCMV SPORT6.1
pDONR223
pEXP4-DEST
Reading Frame Cassette A
pCOLA-2-DEST
pEF-DEST51
pHELLSGATE
Reading Frame Cassette B
pCOLA-3-DEST
pEF5 FRT V5-DEST
pHELLSGATE 12
Reading Frame Cassette C.1
pDEST R4-R3
pENTR GeneBLAzer

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free