pNGS FOS (linearized)

Linearized and dephosphorylated fosmid vector, for stable cloning of large inserts during the creation of genomic libraries for next-generation sequencing. 

Sequence Author: Lucigen

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Lucigen Vectors | More Plasmid Sets
No matches
Read 1 Primer (7703 .. 7735) Illumina Primer A (7678 .. 7697) 454 Primer A (7648 .. 7677) NotI (7642) SL1 (7594 .. 7614) Read 2 Primer (4 .. 40) Illumina Primer B (44 .. 64) 454 Primer B (75 .. 94) NotI (95) SR4 (132 .. 152) pNGS FOS 7738 bp
NotI  (7642)
2 sites
G C G G C C G C C G C C G G C G
NotI  (95)
2 sites
G C G G C C G C C G C C G G C G
Read 1 Primer
33-mer  /  52% GC
1 binding site
7703 .. 7735  =  33 annealed bases
Tm  =  68°C
Illumina Primer A
20-mer  /  55% GC
1 binding site
7678 .. 7697  =  20 annealed bases
Tm  =  60°C
454 Primer A
30-mer  /  60% GC
1 binding site
7648 .. 7677  =  30 annealed bases
Tm  =  68°C
SL1
21-mer  /  57% GC
1 binding site
7594 .. 7614  =  21 annealed bases
Tm  =  59°C
Read 2 Primer
37-mer  /  59% GC
1 binding site
4 .. 40  =  37 annealed bases
Tm  =  74°C
Illumina Primer B
21-mer  /  52% GC
1 binding site
44 .. 64  =  21 annealed bases
Tm  =  58°C
454 Primer B
20-mer  /  60% GC
1 binding site
75 .. 94  =  20 annealed bases
Tm  =  59°C
SR4
21-mer  /  33% GC
1 binding site
132 .. 152  =  21 annealed bases
Tm  =  52°C
sopA
3259 .. 4434  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
sopA
3259 .. 4434  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
repE
5013 .. 5768  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
repE
5013 .. 5768  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
CmR
302 .. 961  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
302 .. 961  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
oriV
6154 .. 6768  =  615 bp
origin of replication for the bacterial F plasmid
oriV
6154 .. 6768  =  615 bp
origin of replication for the bacterial F plasmid
sopC
1742 .. 2215  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
sopC
1742 .. 2215  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
cos
1083 .. 1481  =  399 bp
lambda cos site; allows packaging into phage lambda particles
cos
1083 .. 1481  =  399 bp
lambda cos site; allows packaging into phage lambda particles
incC
4760 .. 5010  =  251 bp
incompatibility region of the bacterial F plasmid
incC
4760 .. 5010  =  251 bp
incompatibility region of the bacterial F plasmid
ori2
5859 .. 6078  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
ori2
5859 .. 6078  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
cat promoter
199 .. 301  =  103 bp
promoter of the E. coli cat gene
cat promoter
199 .. 301  =  103 bp
promoter of the E. coli cat gene
rrnB T1 terminator
150 .. 195  =  46 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
150 .. 195  =  46 bp
transcription terminator T1 from the E. coli rrnB gene
loxP
1032 .. 1065  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
1032 .. 1065  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
tonB terminator
975 .. 1006  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
tonB terminator
975 .. 1006  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
T7Te terminator
7535 .. 7562  =  28 bp
phage T7 early transcription terminator
T7Te terminator
7535 .. 7562  =  28 bp
phage T7 early transcription terminator
T7 promoter
108 .. 126  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
108 .. 126  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
7621 .. 7639  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
7621 .. 7639  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
sopB
2288 .. 3259  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
sopB
2288 .. 3259  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
ORF:  302 .. 961  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  7286 .. 7630  =  345 bp
ORF:  114 amino acids  =  13.0 kDa
ORF:  2982 .. 3218  =  237 bp
ORF:  78 amino acids  =  8.3 kDa
ORF:  5379 .. 5612  =  234 bp
ORF:  77 amino acids  =  9.1 kDa
ORF:  2288 .. 3259  =  972 bp
ORF:  323 amino acids  =  35.4 kDa
ORF:  5219 .. 5464  =  246 bp
ORF:  81 amino acids  =  8.9 kDa
ORF:  5549 .. 5779  =  231 bp
ORF:  76 amino acids  =  8.7 kDa
ORF:  7163 .. 7510  =  348 bp
ORF:  115 amino acids  =  12.9 kDa
ORF:  2509 .. 2907  =  399 bp
ORF:  132 amino acids  =  15.6 kDa
ORF:  3259 .. 4434  =  1176 bp
ORF:  391 amino acids  =  44.1 kDa
ORF:  5013 .. 5768  =  756 bp
ORF:  251 amino acids  =  29.4 kDa
Click here to try SnapGene

Download pNGS FOS (linearized).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.