pCEP4

Episomal mammalian vector with a CMV promoter and a hygromycin resistance marker, for high-level expression in primate and canine cells.

Sequence Author: Thermo Fisher (Invitrogen)

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HomePlasmidsMammalian Expression VectorspCEP4
BsrGI (10,148) AsiSI (8773) PshAI (8436) BstBI (8260) NruI (7959) XmnI (6358) BstEII (5867) EcoNI (5862) StuI * (5791) AvrII (5502) SnaBI (359) Acc65I (620) KpnI (624) PvuII (627) HindIII (636) NotI (647) PaeR7I - PspXI - XhoI (653) NgoMIV (659) NaeI (661) SfiI (665) BamHI (671) EcoRV (1661) NsiI (3212) CsiI - SexAI * (3386) BbvCI (3827) SgrAI (4028) pCEP4 10,186 bp
BsrGI  (10,148)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
AsiSI  (8773)
1 site		 G C G A T C G C C G C T A G C G
PshAI  (8436)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BstBI  (8260)
1 site		 T T C G A A A A G C T T
NruI  (7959)
1 site		 T C G C G A A G C G C T
XmnI  (6358)
1 site		 G A A N N N N T T C C T T N N N N A A G
BstEII  (5867)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
EcoNI  (5862)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
StuI  (5791)
1 site		 A G G C C T T C C G G A
* Blocked by Dcm methylation.
AvrII  (5502)
1 site		 C C T A G G G G A T C C
SnaBI  (359)
1 site		 T A C G T A A T G C A T
Acc65I  (620)
1 site		 G G T A C C C C A T G G
KpnI  (624)
1 site		 G G T A C C C C A T G G
PvuII  (627)
1 site		 C A G C T G G T C G A C
HindIII  (636)
1 site		 A A G C T T T T C G A A
NotI  (647)
1 site		 G C G G C C G C C G C C G G C G
PaeR7I  (653)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (653)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (653)
1 site		 C T C G A G G A G C T C
NgoMIV  (659)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (661)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
SfiI  (665)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BamHI  (671)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRV  (1661)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NsiI  (3212)
1 site		 A T G C A T T A C G T A
CsiI  (3386)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (3386)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BbvCI  (3827)
1 site		 C C T C A G C G G A G T C G
SgrAI  (4028)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
EBNA1
3620 .. 5545  =  1926 bp
641 amino acids  =  56.4 kDa
Product: Epstein-Barr nuclear antigen 1, also known as EBNA-1
crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)
EBNA1
3620 .. 5545  =  1926 bp
641 amino acids  =  56.4 kDa
Product: Epstein-Barr nuclear antigen 1, also known as EBNA-1
crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)
oriP
1529 .. 3318  =  1790 bp
Epstein-Barr virus oriP replication origin (Yates et al., 2000)
oriP
1529 .. 3318  =  1790 bp
Epstein-Barr virus oriP replication origin (Yates et al., 2000)
HygR
8409 .. 9446  =  1038 bp
345 amino acids  =  38.5 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
HygR
8409 .. 9446  =  1038 bp
345 amino acids  =  38.5 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
AmpR
6171 .. 7031  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   6171 .. 6239  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6171 .. 7031  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   6240 .. 7031  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6171 .. 7031  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
7202 .. 7790  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7202 .. 7790  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
4 .. 383  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
4 .. 383  =  380 bp
human cytomegalovirus immediate early enhancer
CMV promoter
384 .. 587  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
384 .. 587  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
HSV TK promoter
8221 .. 8366  =  146 bp
herpes simplex virus thymidine kinase promoter
HSV TK promoter
8221 .. 8366  =  146 bp
herpes simplex virus thymidine kinase promoter
SV40 poly(A) signal
672 .. 806  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
672 .. 806  =  135 bp
SV40 polyadenylation signal
AmpR promoter
6066 .. 6170  =  105 bp
AmpR promoter
6066 .. 6170  =  105 bp
HSV TK poly(A) signal
9488 .. 9536  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
9488 .. 9536  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
MCS
620 .. 676  =  57 bp
multiple cloning site
MCS
620 .. 676  =  57 bp
multiple cloning site
ORF:  4333 .. 5547  =  1215 bp
ORF:  404 amino acids  =  45.2 kDa
ORF:  9736 .. 10,029  =  294 bp
ORF:  97 amino acids  =  11.1 kDa
ORF:  1061 .. 1471  =  411 bp
ORF:  136 amino acids  =  14.7 kDa
ORF:  4142 .. 4378  =  237 bp
ORF:  78 amino acids  =  8.9 kDa
ORF:  7760 .. 8092  =  333 bp
ORF:  110 amino acids  =  12.3 kDa
ORF:  9743 .. 10,051  =  309 bp
ORF:  102 amino acids  =  11.7 kDa
ORF:  6171 .. 7031  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  8409 .. 9446  =  1038 bp
ORF:  345 amino acids  =  38.5 kDa
ORF:  3620 .. 5545  =  1926 bp
ORF:  641 amino acids  =  56.4 kDa
ORF:  6635 .. 6901  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  7709 .. 7945  =  237 bp
ORF:  78 amino acids  =  8.9 kDa
ORF:  1021 .. 1254  =  234 bp
ORF:  77 amino acids  =  8.3 kDa
ORF:  3220 .. 3537  =  318 bp
ORF:  105 amino acids  =  11.7 kDa
ORF:  4318 .. 5430  =  1113 bp
ORF:  370 amino acids  =  40.7 kDa
ORF:  7465 .. 7863  =  399 bp
ORF:  132 amino acids  =  14.8 kDa
ORF:  7927 .. 8205  =  279 bp
ORF:  92 amino acids  =  10.5 kDa
ORF:  2241 .. 2480  =  240 bp
ORF:  79 amino acids  =  8.7 kDa
ORF:  8070 .. 9737  =  1668 bp
ORF:  555 amino acids  =  60.8 kDa
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Individual Sequences & Maps

p3xFLAG-CMV-10
pcDNA6 myc-His A
pCMV6-AC-YFP
pHet-Act1-2
p3xFLAG-CMV-13
pcDNA6 myc-His B
pCMV6-AN-3DDK
pHet-Mem1
p3xFLAG-CMV-14
pcDNA6 myc-His C
pCMV6-AN-3HA
pHet-Nuc1
p3xFLAG-CMV-7
pcDNA6 TR
pCMV6-AN-DDK
pHom-1
p3xFLAG-CMV-7.1
pcDNA6 V5-His A
pCMV6-AN-FC
pHom-Mem1
p3xFLAG-CMV-8
pcDNA6 V5-His B
pCMV6-AN-FC-S
pHTC HaloTag CMV-neo
p3xFLAG-CMV-9
pcDNA6 V5-His C
pCMV6-AN-FP602
pHTN HaloTag CMV-neo
p3xFLAG-Myc-CMV-23
pCEP4
pCMV6-AN-FP635
pIRES
p3xFLAG-Myc-CMV-24
pCI
pCMV6-AN-GFP
pIRES2 DsRed-Express2
p3xFLAG-Myc-CMV-25
pCI-neo
pCMV6-AN-GFP-AC-His
pIRES2-AcGFP1
p3xFLAG-Myc-CMV-26
pCMV SPORT6
pCMV6-AN-HA
pIRES2-EGFP
pALTER-MAX
pCMV SPORT6.1
pCMV6-AN-His
pIRES2-ZsGreen1
pBApo-CMV
pCMV-(DYKDDDDK)-C
pCMV6-AN-His-DDK
pIRESbleo3
pBApo-CMV Neo
pCMV-(DYKDDDDK)-N
pCMV6-AN-His-HA
pIREShyg3
pBApo-CMV Pur
pCMV-3Tag-1A
pCMV6-AN-His-Myc
pIRESneo3
pBApo-EF1(alpha) Neo
pCMV-3Tag-1B
pCMV6-AN-mBFP
pIRESpuro3
pBApo-EF1(alpha) Pur
pCMV-3Tag-1C
pCMV6-AN-mCFP
pM-secSUMOstar
pBI-CMV1
pCMV-3Tag-2A
pCMV6-AN-mGFP
pM-SUMOstar
pBI-CMV2
pCMV-3Tag-2B
pCMV6-AN-mKate
pME-HA (linearized)
pBI-CMV3
pCMV-3Tag-2C
pCMV6-AN-mRFP
pmRi-mCherry
pBI-CMV4
pCMV-3Tag-3A
pCMV6-AN-Myc
pmRi-ZsGreen1
pBK-CMV
pCMV-3Tag-3B
pCMV6-AN-Myc-DDK
pNTAP-A
pBudCE4.1
pCMV-3Tag-3C
pCMV6-AN-mYFP
pNTAP-B
pcDNA3
pCMV-3Tag-4A
pCMV6-AN-RFP
pNTAP-C
pcDNA3.1+ C-DYK
pCMV-3Tag-4B
pCMV6-AN-YFP
pOG44
pcDNA3.1+ C-HA
pCMV-3Tag-4C
pCMV6-Entry
pPTuner
pcDNA3.1+ C-His
pCMV-3Tag-6
pCMV6-Entry-Cre
pPTuner IRES2
pcDNA3.1+ C-Myc
pCMV-3Tag-7
pCMV6-Entry2
pPTunerC
pcDNA3.1+ N-GST(TEV)
pCMV-3Tag-8
pCMV6-XL5-DDK-His
pREP4
pcDNA3.1+ N-GST(Thrombin)
pCMV-3Tag-9
pCS6-Empty
prHom-1
pcDNA3.1+ N-HA
pCMV-DDK-C
pCTAP-A
prHom-Nuc1
pcDNA3.1+ N-His
pCMV-DDK-N
pCTAP-B
prHom-Sec1
pcDNA3.1+ N-Myc
pCMV-HA
pCTAP-C
pSecTag A
pcDNA-DEST40
pCMV-HA-C
pDEST26
pSecTag B
pcDNA-DEST47
pCMV-HA-N
pDEST27
pSecTag C
pcDNA-DEST53
pCMV-LacZ
pDisplay
pSecTag2 A
pcDNA3.1(+)
pCMV-MIR
pEF-DEST51
pSecTag2 B
pcDNA3.1(-)
pCMV-Myc
pEF1alpha-IRES
pSecTag2 C
pcDNA3.1 CT-GFP
pCMV-Myc-C
pEF1alpha-IRES-AcGFP1
pSI
pcDNA3.1 His A
pCMV-Myc-N
pEF1alpha-IRES-DsRed-Express2
pSingle-tTs-shRNA
pcDNA3.1 His B
pCMV-Script
pEF1alpha-IRES-ZsGreen1
pT-REx-DEST30
pcDNA3.1 His C
pCMV-Script EX
pEF1alpha-Tet3G
pT-REx-DEST31
pcDNA3.1 Hygro(+)
pCMV-Tag 1
pEF5 FRT V5-DEST
pTandem-1
pcDNA3.1 Hygro(-)
pCMV-Tag 2A
pEF6 V5-His A
pTCN
pcDNA3.1 myc-His A
pCMV-Tag 2B
pEF6 V5-His B
pTCP
pcDNA3.1 myc-His B
pCMV-Tag 2C
pEF6 V5-His C
pTet-DualOFF
pcDNA3.1 myc-His C
pCMV-Tag 3A
pEntry-rtTA
pTet-DualON
pcDNA3.1 myc-His(-) A
pCMV-Tag 3B
pF4A CMV
pTet-Off
pcDNA3.1 myc-His(-) B
pCMV-Tag 3C
pF4K CMV
pTet-Off-Advanced
pcDNA3.1 myc-His(-) C
pCMV-Tag 4A
pF5A CMV-neo
pTet-On
pcDNA3.1 NT-GFP
pCMV-Tag 4B
pF5K CMV-neo
pTet-On-Advanced
pcDNA3.1 nV5-DEST
pCMV-Tag 4C
pFC14A HaloTag CMV
pTet-tTS
pcDNA3.1 V5-His A
pCMV-Tag 5A
pFC14K HaloTag CMV
pTetOne
pcDNA3.1 V5-His B
pCMV-Tag 5B
pFC15A HaloTag CMVd1
pTK-neo
pcDNA3.1 V5-His C
pCMV-Tag 5C
pFC15K HaloTag CMVd1
pTracer-CMV
pcDNA3.1 Zeo(+)
pCMV-Tet3G
pFC16A HaloTag CMVd2
pTracer-CMV Bsd
pcDNA3.1 Zeo(-)
pCMV6-A-BSD
pFC16K HaloTag CMVd2
pTracer-CMV2
pcDNA3.2 V5-DEST
pCMV6-A-EM7-BSD
pFC17A HaloTag CMVd3
pTracer-EF V5-His A
pcDNA4 His A
pCMV6-A-GFP
pFC17K HaloTag CMVd3
pTracer-EF V5-His B
pcDNA4 His B
pCMV6-A-Hygro
pFC27A HaloTag CMV-neo
pTracer-EF V5-His C
pcDNA4 His C
pCMV6-A-Puro
pFC27K HaloTag CMV-neo
pTracer-SV40
pcDNA4 HisMax A
pCMV6-AC
pFLAG-CMV-1
pTRE-Cycle1
pcDNA4 HisMax B
pCMV6-AC-3DDK
pFLAG-CMV-2
pTRE-Cycle2
pcDNA4 HisMax C
pCMV6-AC-3HA
pFLAG-CMV-3
pTRE-Cycle3
pcDNA4 myc-His A
pCMV6-AC-DDK
pFLAG-CMV-4
pTRE-Dual1
pcDNA4 myc-His B
pCMV6-AC-DDK-His
pFLAG-CMV-5.1
pTRE-Dual2
pcDNA4 myc-His C
pCMV6-AC-FC
pFLAG-Myc-CMV-19
pTRE-Tight
pcDNA4 TO
pCMV6-AC-FC-S
pFLAG-Myc-CMV-20
pTRE-Tight-BI
pcDNA4 TO myc-His A
pCMV6-AC-FP602
pFLAG-Myc-CMV-21
pTRE-Tight-BI-AcGFP1
pcDNA4 TO myc-His B
pCMV6-AC-FP635
pFLAG-Myc-CMV-22
pTRE-Tight-BI-DsRed-Express
pcDNA4 TO myc-His C
pCMV6-AC-GFP
pFN21A HaloTag CMV
pTRE-Tight-BI-ZsGreen1
pcDNA4 V5-His A
pCMV6-AC-GFP-rtTA
pFN21K HaloTag CMV
pTRE2
pcDNA4 V5-His B
pCMV6-AC-HA
pFN22A HaloTag CMVd1
pTRE2hyg
pcDNA4 V5-His C
pCMV6-AC-HA-His
pFN22K HaloTag CMVd1
pTRE2pur
pcDNA5 FRT
pCMV6-AC-His
pFN23A HaloTag CMVd2
pTRE3G
pcDNA5 FRT TO
pCMV6-AC-IRES-GFP
pFN23K HaloTag CMVd2
pTRE3G-BI
pcDNA5 TO
pCMV6-AC-IRES-GFP-Puro
pFN24A HaloTag CMVd3
pTRE3G-BI-Het1
pcDNA6.2 C-EmGFP-DEST
pCMV6-AC-mBFP
pFN24K HaloTag CMVd3
pTRE3G-BI-mCherry
pcDNA6.2 C-YFP-DEST
pCMV6-AC-mCFP
pFN28A HaloTag CMV-neo
pTRE3G-BI-ZsGreen1
pcDNA6.2 cGeneBLAzer-DEST
pCMV6-AC-mGFP
pFN28K HaloTag CMV-neo
pTRE3G-IRES
pcDNA6.2 cLumio-DEST
pCMV6-AC-mKate
pFRT lacZeo
pTRE3G-mCherry
pcDNA6.2 EmGFP-Bsd V5-DEST
pCMV6-AC-mRFP
pFRT lacZeo2
pTRE3G-ZsGreen1
pcDNA6.2 N-EmGFP-DEST
pCMV6-AC-Myc
pGen2.1
pVAX1
pcDNA6.2 N-YFP-DEST
pCMV6-AC-Myc-DDK
phCMV1
pX330
pcDNA6.2 nGeneBLAzer-DEST
pCMV6-AC-Myc-His
phCMV2
pZeoSV2(+)
pcDNA6.2 nLumio-DEST
pCMV6-AC-mYFP
phCMV3
pZeoSV2(-)
pcDNA6.2 V5-DEST
pCMV6-AC-RFP
pHet-1
pZFHD1-2
pcDNA6.2 V5-pL-DEST

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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