pTRE2hyg

Expression vector with a Tet-responsive promoter and a hygromycin resistance marker.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsMammalian Expression VectorspTRE2hyg
SV40 promoter SfiI (5073) AvrII (5019) PshAI (4972) BfuAI - BspMI (4682) PstI (4666) AsiSI (4637) RsrII (4588) BtgZI (4323) BsaBI * (3959) BsmI (3872) HpaI (3858) XmnI (3378) FspI (3001) BsaI (2840) AhdI (2779) CsiI - SexAI * (5251) BamHI (471) PvuII (485) MluI (489) NheI (495) BmtI (499) NotI (502) BspDI - ClaI (510) SalI (521) AccI (522) EcoRV (529) KflI - PpuMI (564) PspOMI (1081) ApaI (1085) Bsu36I (1185) BsgI (1211) MscI (1226) BstXI (1234) BglII (1255) PflMI (1462) BspQI - SapI (1770) pTRE2hyg 5337 bp
SfiI  (5073)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AvrII  (5019)
1 site		 C C T A G G G G A T C C
PshAI  (4972)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BfuAI  (4682)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (4682)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PstI  (4666)
1 site		 C T G C A G G A C G T C
AsiSI  (4637)
1 site		 G C G A T C G C C G C T A G C G
RsrII  (4588)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BtgZI  (4323)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsaBI  (3959)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BsmI  (3872)
1 site		 G A A T G C N C T T A C G N
Sticky ends from different BsmI sites may not be compatible.
HpaI  (3858)
1 site		 G T T A A C C A A T T G
XmnI  (3378)
1 site		 G A A N N N N T T C C T T N N N N A A G
FspI  (3001)
1 site		 T G C G C A A C G C G T
BsaI  (2840)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2779)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
CsiI  (5251)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (5251)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BamHI  (471)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PvuII  (485)
1 site		 C A G C T G G T C G A C
MluI  (489)
1 site		 A C G C G T T G C G C A
NheI  (495)
1 site		 G C T A G C C G A T C G
BmtI  (499)
1 site		 G C T A G C C G A T C G
NotI  (502)
1 site		 G C G G C C G C C G C C G G C G
BspDI  (510)
1 site		 A T C G A T T A G C T A
ClaI  (510)
1 site		 A T C G A T T A G C T A
SalI  (521)
1 site		 G T C G A C C A G C T G
AccI  (522)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
EcoRV  (529)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
KflI  (564)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
PpuMI  (564)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
PspOMI  (1081)
1 site		 G G G C C C C C C G G G
ApaI  (1085)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
Bsu36I  (1185)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
BsgI  (1211)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MscI  (1226)
1 site		 T G G C C A A C C G G T
BstXI  (1234)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
BglII  (1255)
1 site		 A G A T C T T C T A G A
PflMI  (1462)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
BspQI  (1770)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (1770)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
HygR
3975 .. 5000  =  1026 bp
341 amino acids  =  38.0 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
HygR
3975 .. 5000  =  1026 bp
341 amino acids  =  38.0 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
AmpR
2706 .. 3566  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   2706 .. 3497  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2706 .. 3566  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   3498 .. 3566  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2706 .. 3566  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1947 .. 2535  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1947 .. 2535  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
β-globin intron
554 .. 1126  =  573 bp
intron from rabbit β-globin gene
β-globin intron
554 .. 1126  =  573 bp
intron from rabbit β-globin gene
SV40 promoter
5009 .. 1  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
5009 .. 1  =  330 bp
SV40 enhancer and early promoter
tetracycline response element
15 .. 285  =  271 bp
13 segments
   Segment 1:  tetO  
   15 .. 33  =  19 bp
contains seven copies of the tetracycline operator tetO
tetracycline response element
15 .. 285  =  271 bp
13 segments
   Segment 2:  
   34 .. 56  =  23 bp
contains seven copies of the tetracycline operator tetO
tetracycline response element
15 .. 285  =  271 bp
13 segments
   Segment 3:  tetO  
   57 .. 75  =  19 bp
contains seven copies of the tetracycline operator tetO
tetracycline response element
15 .. 285  =  271 bp
13 segments
   Segment 4:  
   76 .. 98  =  23 bp
contains seven copies of the tetracycline operator tetO
tetracycline response element
15 .. 285  =  271 bp
13 segments
   Segment 5:  tetO  
   99 .. 117  =  19 bp
contains seven copies of the tetracycline operator tetO
tetracycline response element
15 .. 285  =  271 bp
13 segments
   Segment 6:  
   118 .. 140  =  23 bp
contains seven copies of the tetracycline operator tetO
tetracycline response element
15 .. 285  =  271 bp
13 segments
   Segment 7:  tetO  
   141 .. 159  =  19 bp
contains seven copies of the tetracycline operator tetO
tetracycline response element
15 .. 285  =  271 bp
13 segments
   Segment 8:  
   160 .. 182  =  23 bp
contains seven copies of the tetracycline operator tetO
tetracycline response element
15 .. 285  =  271 bp
13 segments
   Segment 9:  tetO  
   183 .. 201  =  19 bp
contains seven copies of the tetracycline operator tetO
tetracycline response element
15 .. 285  =  271 bp
13 segments
   Segment 10:  
   202 .. 224  =  23 bp
contains seven copies of the tetracycline operator tetO
tetracycline response element
15 .. 285  =  271 bp
13 segments
   Segment 11:  tetO  
   225 .. 243  =  19 bp
contains seven copies of the tetracycline operator tetO
tetracycline response element
15 .. 285  =  271 bp
13 segments
   Segment 12:  
   244 .. 266  =  23 bp
contains seven copies of the tetracycline operator tetO
tetracycline response element
15 .. 285  =  271 bp
13 segments
   Segment 13:  tetO  
   267 .. 285  =  19 bp
contains seven copies of the tetracycline operator tetO
tetracycline response element
15 .. 285  =  271 bp
13 segments
contains seven copies of the tetracycline operator tetO
AmpR promoter
3567 .. 3671  =  105 bp
AmpR promoter
3567 .. 3671  =  105 bp
SV40 poly(A) signal
3777 .. 3858  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3777 .. 3858  =  82 bp
SV40 polyadenylation signal
MCS
471 .. 532  =  62 bp
multiple cloning site
MCS
471 .. 532  =  62 bp
multiple cloning site
β-globin poly(A) signal
1323 .. 1378  =  56 bp
rabbit β-globin polyadenylation signal
β-globin poly(A) signal
1323 .. 1378  =  56 bp
rabbit β-globin polyadenylation signal
minimal CMV promoter
318 .. 356  =  39 bp
human cytomegalovirus (CMV) immediate early promoter
minimal CMV promoter
318 .. 356  =  39 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 ori
5023 .. 5158  =  136 bp
SV40 origin of replication
SV40 ori
5023 .. 5158  =  136 bp
SV40 origin of replication
ORF:  2836 .. 3102  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3949 .. 4176  =  228 bp
ORF:  75 amino acids  =  8.0 kDa
ORF:  4242 .. 5093  =  852 bp
ORF:  283 amino acids  =  31.8 kDa
ORF:  2706 .. 3566  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  3975 .. 5000  =  1026 bp
ORF:  341 amino acids  =  38.0 kDa
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Individual Sequences & Maps

p3xFLAG-CMV-10
pcDNA6 myc-His A
pCMV6-AC-YFP
pHet-Act1-2
p3xFLAG-CMV-13
pcDNA6 myc-His B
pCMV6-AN-3DDK
pHet-Mem1
p3xFLAG-CMV-14
pcDNA6 myc-His C
pCMV6-AN-3HA
pHet-Nuc1
p3xFLAG-CMV-7
pcDNA6 TR
pCMV6-AN-DDK
pHom-1
p3xFLAG-CMV-7.1
pcDNA6 V5-His A
pCMV6-AN-FC
pHom-Mem1
p3xFLAG-CMV-8
pcDNA6 V5-His B
pCMV6-AN-FC-S
pHTC HaloTag CMV-neo
p3xFLAG-CMV-9
pcDNA6 V5-His C
pCMV6-AN-FP602
pHTN HaloTag CMV-neo
p3xFLAG-Myc-CMV-23
pCEP4
pCMV6-AN-FP635
pIRES
p3xFLAG-Myc-CMV-24
pCI
pCMV6-AN-GFP
pIRES2 DsRed-Express2
p3xFLAG-Myc-CMV-25
pCI-neo
pCMV6-AN-GFP-AC-His
pIRES2-AcGFP1
p3xFLAG-Myc-CMV-26
pCMV SPORT6
pCMV6-AN-HA
pIRES2-EGFP
pALTER-MAX
pCMV SPORT6.1
pCMV6-AN-His
pIRES2-ZsGreen1
pBApo-CMV
pCMV-(DYKDDDDK)-C
pCMV6-AN-His-DDK
pIRESbleo3
pBApo-CMV Neo
pCMV-(DYKDDDDK)-N
pCMV6-AN-His-HA
pIREShyg3
pBApo-CMV Pur
pCMV-3Tag-1A
pCMV6-AN-His-Myc
pIRESneo3
pBApo-EF1(alpha) Neo
pCMV-3Tag-1B
pCMV6-AN-mBFP
pIRESpuro3
pBApo-EF1(alpha) Pur
pCMV-3Tag-1C
pCMV6-AN-mCFP
pM-secSUMOstar
pBI-CMV1
pCMV-3Tag-2A
pCMV6-AN-mGFP
pM-SUMOstar
pBI-CMV2
pCMV-3Tag-2B
pCMV6-AN-mKate
pME-HA (linearized)
pBI-CMV3
pCMV-3Tag-2C
pCMV6-AN-mRFP
pmRi-mCherry
pBI-CMV4
pCMV-3Tag-3A
pCMV6-AN-Myc
pmRi-ZsGreen1
pBK-CMV
pCMV-3Tag-3B
pCMV6-AN-Myc-DDK
pNTAP-A
pBudCE4.1
pCMV-3Tag-3C
pCMV6-AN-mYFP
pNTAP-B
pcDNA3
pCMV-3Tag-4A
pCMV6-AN-RFP
pNTAP-C
pcDNA3.1+ C-DYK
pCMV-3Tag-4B
pCMV6-AN-YFP
pOG44
pcDNA3.1+ C-HA
pCMV-3Tag-4C
pCMV6-Entry
pPTuner
pcDNA3.1+ C-His
pCMV-3Tag-6
pCMV6-Entry-Cre
pPTuner IRES2
pcDNA3.1+ C-Myc
pCMV-3Tag-7
pCMV6-Entry2
pPTunerC
pcDNA3.1+ N-GST(TEV)
pCMV-3Tag-8
pCMV6-XL5-DDK-His
pREP4
pcDNA3.1+ N-GST(Thrombin)
pCMV-3Tag-9
pCS6-Empty
prHom-1
pcDNA3.1+ N-HA
pCMV-DDK-C
pCTAP-A
prHom-Nuc1
pcDNA3.1+ N-His
pCMV-DDK-N
pCTAP-B
prHom-Sec1
pcDNA3.1+ N-Myc
pCMV-HA
pCTAP-C
pSecTag A
pcDNA-DEST40
pCMV-HA-C
pDEST26
pSecTag B
pcDNA-DEST47
pCMV-HA-N
pDEST27
pSecTag C
pcDNA-DEST53
pCMV-LacZ
pDisplay
pSecTag2 A
pcDNA3.1(+)
pCMV-MIR
pEF-DEST51
pSecTag2 B
pcDNA3.1(-)
pCMV-Myc
pEF1alpha-IRES
pSecTag2 C
pcDNA3.1 CT-GFP
pCMV-Myc-C
pEF1alpha-IRES-AcGFP1
pSI
pcDNA3.1 His A
pCMV-Myc-N
pEF1alpha-IRES-DsRed-Express2
pSingle-tTs-shRNA
pcDNA3.1 His B
pCMV-Script
pEF1alpha-IRES-ZsGreen1
pT-REx-DEST30
pcDNA3.1 His C
pCMV-Script EX
pEF1alpha-Tet3G
pT-REx-DEST31
pcDNA3.1 Hygro(+)
pCMV-Tag 1
pEF5 FRT V5-DEST
pTandem-1
pcDNA3.1 Hygro(-)
pCMV-Tag 2A
pEF6 V5-His A
pTCN
pcDNA3.1 myc-His A
pCMV-Tag 2B
pEF6 V5-His B
pTCP
pcDNA3.1 myc-His B
pCMV-Tag 2C
pEF6 V5-His C
pTet-DualOFF
pcDNA3.1 myc-His C
pCMV-Tag 3A
pEntry-rtTA
pTet-DualON
pcDNA3.1 myc-His(-) A
pCMV-Tag 3B
pF4A CMV
pTet-Off
pcDNA3.1 myc-His(-) B
pCMV-Tag 3C
pF4K CMV
pTet-Off-Advanced
pcDNA3.1 myc-His(-) C
pCMV-Tag 4A
pF5A CMV-neo
pTet-On
pcDNA3.1 NT-GFP
pCMV-Tag 4B
pF5K CMV-neo
pTet-On-Advanced
pcDNA3.1 nV5-DEST
pCMV-Tag 4C
pFC14A HaloTag CMV
pTet-tTS
pcDNA3.1 V5-His A
pCMV-Tag 5A
pFC14K HaloTag CMV
pTetOne
pcDNA3.1 V5-His B
pCMV-Tag 5B
pFC15A HaloTag CMVd1
pTK-neo
pcDNA3.1 V5-His C
pCMV-Tag 5C
pFC15K HaloTag CMVd1
pTracer-CMV
pcDNA3.1 Zeo(+)
pCMV-Tet3G
pFC16A HaloTag CMVd2
pTracer-CMV Bsd
pcDNA3.1 Zeo(-)
pCMV6-A-BSD
pFC16K HaloTag CMVd2
pTracer-CMV2
pcDNA3.2 V5-DEST
pCMV6-A-EM7-BSD
pFC17A HaloTag CMVd3
pTracer-EF V5-His A
pcDNA4 His A
pCMV6-A-GFP
pFC17K HaloTag CMVd3
pTracer-EF V5-His B
pcDNA4 His B
pCMV6-A-Hygro
pFC27A HaloTag CMV-neo
pTracer-EF V5-His C
pcDNA4 His C
pCMV6-A-Puro
pFC27K HaloTag CMV-neo
pTracer-SV40
pcDNA4 HisMax A
pCMV6-AC
pFLAG-CMV-1
pTRE-Cycle1
pcDNA4 HisMax B
pCMV6-AC-3DDK
pFLAG-CMV-2
pTRE-Cycle2
pcDNA4 HisMax C
pCMV6-AC-3HA
pFLAG-CMV-3
pTRE-Cycle3
pcDNA4 myc-His A
pCMV6-AC-DDK
pFLAG-CMV-4
pTRE-Dual1
pcDNA4 myc-His B
pCMV6-AC-DDK-His
pFLAG-CMV-5.1
pTRE-Dual2
pcDNA4 myc-His C
pCMV6-AC-FC
pFLAG-Myc-CMV-19
pTRE-Tight
pcDNA4 TO
pCMV6-AC-FC-S
pFLAG-Myc-CMV-20
pTRE-Tight-BI
pcDNA4 TO myc-His A
pCMV6-AC-FP602
pFLAG-Myc-CMV-21
pTRE-Tight-BI-AcGFP1
pcDNA4 TO myc-His B
pCMV6-AC-FP635
pFLAG-Myc-CMV-22
pTRE-Tight-BI-DsRed-Express
pcDNA4 TO myc-His C
pCMV6-AC-GFP
pFN21A HaloTag CMV
pTRE-Tight-BI-ZsGreen1
pcDNA4 V5-His A
pCMV6-AC-GFP-rtTA
pFN21K HaloTag CMV
pTRE2
pcDNA4 V5-His B
pCMV6-AC-HA
pFN22A HaloTag CMVd1
pTRE2hyg
pcDNA4 V5-His C
pCMV6-AC-HA-His
pFN22K HaloTag CMVd1
pTRE2pur
pcDNA5 FRT
pCMV6-AC-His
pFN23A HaloTag CMVd2
pTRE3G
pcDNA5 FRT TO
pCMV6-AC-IRES-GFP
pFN23K HaloTag CMVd2
pTRE3G-BI
pcDNA5 TO
pCMV6-AC-IRES-GFP-Puro
pFN24A HaloTag CMVd3
pTRE3G-BI-Het1
pcDNA6.2 C-EmGFP-DEST
pCMV6-AC-mBFP
pFN24K HaloTag CMVd3
pTRE3G-BI-mCherry
pcDNA6.2 C-YFP-DEST
pCMV6-AC-mCFP
pFN28A HaloTag CMV-neo
pTRE3G-BI-ZsGreen1
pcDNA6.2 cGeneBLAzer-DEST
pCMV6-AC-mGFP
pFN28K HaloTag CMV-neo
pTRE3G-IRES
pcDNA6.2 cLumio-DEST
pCMV6-AC-mKate
pFRT lacZeo
pTRE3G-mCherry
pcDNA6.2 EmGFP-Bsd V5-DEST
pCMV6-AC-mRFP
pFRT lacZeo2
pTRE3G-ZsGreen1
pcDNA6.2 N-EmGFP-DEST
pCMV6-AC-Myc
pGen2.1
pVAX1
pcDNA6.2 N-YFP-DEST
pCMV6-AC-Myc-DDK
phCMV1
pX330
pcDNA6.2 nGeneBLAzer-DEST
pCMV6-AC-Myc-His
phCMV2
pZeoSV2(+)
pcDNA6.2 nLumio-DEST
pCMV6-AC-mYFP
phCMV3
pZeoSV2(-)
pcDNA6.2 V5-DEST
pCMV6-AC-RFP
pHet-1
pZFHD1-2
pcDNA6.2 V5-pL-DEST

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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