pBAD202 D-TOPO

Bacterial directional TOPO® cloning vector for arabinose-inducible expression of solubilized and N- and C-terminally tagged proteins.

Sequence Author: Thermo Fisher (Invitrogen)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: TOPO Cloning Vectors | More Plasmid Sets
No matches
HomePlasmidsTOPO Cloning VectorspBAD202 D-TOPO
PflMI (3642) BspEI (3437) NruI (3430) EcoRV (3108) BsaBI (2963) NsiI (2731) MauBI (2707) BsmBI - Esp3I (2641) PfoI (2639) BstZ17I (2515) AccI (2514) NdeI (2463) PciI (2284) AlwNI (1875) EagI (1477) PluTI (1388) BstEII (3799) BsmI (3819) NcoI - StyI (4066) AleI (4163) HP-thioredoxin BspDI * - ClaI * (4252) TspMI - XmaI (4262) SmaI (4264) Eco53kI (9) SacI (11) HindIII (13) BseRI (34) 6xHis BpmI (90) PmeI (97) AclI (422) rrnB T2 terminator BstBI (703) NmeAIII (1165) PvuII (1281) FspI (1285) MscI (1305) PstI (1338) KasI (1384) NarI (1385) SfoI (1386) pBAD202/D-TOPO® 4444 bp
PflMI  (3642)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
BspEI  (3437)
1 site		 T C C G G A A G G C C T
NruI  (3430)
1 site		 T C G C G A A G C G C T
EcoRV  (3108)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BsaBI  (2963)
1 site		 G A T N N N N A T C C T A N N N N T A G
NsiI  (2731)
1 site		 A T G C A T T A C G T A
MauBI  (2707)
1 site		 C G C G C G C G G C G C G C G C
BsmBI  (2641)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (2641)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
PfoI  (2639)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
BstZ17I  (2515)
1 site		 G T A T A C C A T A T G
AccI  (2514)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
NdeI  (2463)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
PciI  (2284)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
AlwNI  (1875)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
EagI  (1477)
1 site		 C G G C C G G C C G G C
PluTI  (1388)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BstEII  (3799)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BsmI  (3819)
1 site		 G A A T G C N C T T A C G N
Sticky ends from different BsmI sites may not be compatible.
NcoI  (4066)
1 site		 C C A T G G G G T A C C
StyI  (4066)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
AleI  (4163)
1 site		 C A C N N N N G T G G T G N N N N C A C
BspDI  (4252)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (4252)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
TspMI  (4262)
1 site		 C C C G G G G G G C C C
XmaI  (4262)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (4264)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
Eco53kI  (9)
1 site		 G A G C T C C T C G A G
SacI  (11)
1 site		 G A G C T C C T C G A G
HindIII  (13)
1 site		 A A G C T T T T C G A A
BseRI  (34)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BpmI  (90)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
PmeI  (97)
1 site		 G T T T A A A C C A A A T T T G
AclI  (422)
1 site		 A A C G T T T T G C A A
BstBI  (703)
1 site		 T T C G A A A A G C T T
NmeAIII  (1165)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PvuII  (1281)
1 site		 C A G C T G G T C G A C
FspI  (1285)
1 site		 T G C G C A A C G C G T
MscI  (1305)
1 site		 T G G C C A A C C G G T
PstI  (1338)
1 site		 C T G C A G G A C G T C
KasI  (1384)
1 site		 G G C G C C C C G C G G
NarI  (1385)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1386)
1 site		 G G C G C C C C G C G G
araC
2818 .. 3696  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
araC
2818 .. 3696  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
NeoR/KanR
722 .. 1516  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
722 .. 1516  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
1640 .. 2228  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1640 .. 2228  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
HP-thioredoxin
4068 .. 4397  =  330 bp
110 amino acids  =  11.9 kDa
Product: His-Patch thioredoxin
The histidines at positions 8, 32, and 64 coordinate a divalent cation, allowing HP-thioredoxin to be purified on metal-chelating resins.
HP-thioredoxin
4068 .. 4397  =  330 bp
110 amino acids  =  11.9 kDa
Product: His-Patch thioredoxin
The histidines at positions 8, 32, and 64 coordinate a divalent cation, allowing HP-thioredoxin to be purified on metal-chelating resins.
enterokinase site
4413 .. 4427  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
4413 .. 4427  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
araBAD promoter
3723 .. 4007  =  285 bp
promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)
araBAD promoter
3723 .. 4007  =  285 bp
promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)
rrnB T1 terminator
316 .. 402  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
316 .. 402  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
V5 tag
22 .. 63  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
22 .. 63  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
6xHis
73 .. 90  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
73 .. 90  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
rrnB T2 terminator
494 .. 521  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
494 .. 521  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
RBS
4051 .. 4056  =  6 bp
ribosome binding site
RBS
4051 .. 4056  =  6 bp
ribosome binding site
ORF:  3382 .. 3630  =  249 bp
ORF:  82 amino acids  =  9.3 kDa
ORF:  953 .. 1207  =  255 bp
ORF:  84 amino acids  =  9.7 kDa
ORF:  4068 .. 4448  =  381 bp
ORF:  127 amino acids  =  13.6 kDa
ORF:  4023 .. 4448  =  426 bp
ORF:  141 amino acids  =  16.3 kDa  (no start codon)
ORF:  722 .. 1516  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  157 .. 483  =  327 bp
ORF:  108 amino acids  =  12.1 kDa
ORF:  958 .. 1344  =  387 bp
ORF:  128 amino acids  =  14.5 kDa
ORF:  2818 .. 3747  =  930 bp
ORF:  309 amino acids  =  35.2 kDa
Click here to try SnapGene

Download pBAD202 D-TOPO.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

pBAD-TOPO (linearized)
pcDNA5 FRT TO-TOPO (linearized)
pEF6 V5-His-TOPO (linearized)
pGlow-TOPO (linearized)
pBAD Thio-TOPO (linearized)
pcDNA5 FRT V5-His-TOPO (linearized)
pENTR D-TOPO
pIB V5-His-TOPO (linearized)
pBAD202 D-TOPO
pcDNA6.2 C-EmGFP-GW TOPO (linearized)
pENTR SD D-TOPO
pLenti6.3 V5-TOPO (linearized)
pBlue-TOPO (linearized)
pcDNA6.2 C-YFP-GW TOPO (linearized)
pENTR TEV D-TOPO
pLenti6 V5-D-TOPO
pcDNA3.1D V5-His-TOPO
pcDNA6.2 N-EmGFP-GW TOPO (linearized)
pENTR5'-TOPO (linearized)
pLenti7.3 V5-TOPO (linearized)
pcDNA3.2 V5 GW D-TOPO
pCR-XL-2-TOPO (linearized)
pET100 D-TOPO
pLPS-B (linearized)
pcDNA6.2 cGeneBLAzer-GW D-TOPO
pCR-XL-TOPO (linearized)
pET101 D-TOPO
pMT V5-His-TOPO (linearized)
pcDNA6.2 V5 GW D-TOPO
pCR2.1-TOPO (linearized)
pET102 D-TOPO
pOptiVEC-TOPO (linearized)
pcDNA3.1 CT-GFP-TOPO (linearized)
pCR4-TOPO (linearized)
pET151 D-TOPO
pSecTag FRT V5-His-TOPO (linearized)
pcDNA3.1 NT-GFP-TOPO (linearized)
pCR4Blunt-TOPO (linearized)
pET160 GW D-TOPO
pTOP Blunt V2 (linearized)
pcDNA3.1 V5-His-TOPO (linearized)
pCR8 GW TOPO (linearized)
pET161 GW D-TOPO
pTrcHis-TOPO (linearized)
pcDNA3.3-TOPO (linearized)
pCR-Blunt II-TOPO (linearized)
pET200 D-TOPO
pTrcHis2-TOPO (linearized)
pcDNA3.4-TOPO (linearized)
pCRII-TOPO (linearized)
pEXP5-CT TOPO (linearized)
pYES2.1 V5-His-TOPO (linearized)
pcDNA4 HisMax-TOPO (linearized)
pEF5 FRT V5-D-TOPO
pEXP5-NT TOPO (linearized)

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free