pYES2.1 V5-His-TOPO (linearized)

Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products and expression of tagged proteins in yeast cells.

Sequence Author: Thermo Fisher (Invitrogen)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: TOPO Cloning Vectors | More Plasmid Sets
No matches
HomePlasmidsTOPO Cloning VectorspYES2.1 V5-His-TOPO (linearized)
NaeI (5229) NgoMIV (5227) SwaI (4901) SnaBI (4597) AvaI - BsoBI (4247) HpaI (3952) BmgBI (3892) PfoI * (3850) NruI (3546) BlpI (3510) BspDI - ClaI (3366) BmtI (3361) NheI (3357) NdeI (3217) BsgI (3188) SbfI (3154) BfuAI - BspMI (3143) XcmI (3005) EcoRV (2944) NcoI (2923) BsmI (2772) ApaI * (2758) PspOMI * (2754) BsmBI - Esp3I (2506) SpeI (5369) PvuII (5845) End (5886) Start (1) BstEII (16) XbaI (106) BsrGI (285) BciVI (757) BspHI (1274) AhdI (1447) BglI (1567) BsaHI (1984) pYES2.1/V5-His-TOPO® 5885 bp
NaeI  (5229)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (5227)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SwaI  (4901)
1 site		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
SnaBI  (4597)
1 site		 T A C G T A A T G C A T
AvaI  (4247)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (4247)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
HpaI  (3952)
1 site		 G T T A A C C A A T T G
BmgBI  (3892)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
PfoI  (3850)
1 site		 T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
NruI  (3546)
1 site		 T C G C G A A G C G C T
BlpI  (3510)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
BspDI  (3366)
1 site		 A T C G A T T A G C T A
ClaI  (3366)
1 site		 A T C G A T T A G C T A
BmtI  (3361)
1 site		 G C T A G C C G A T C G
NheI  (3357)
1 site		 G C T A G C C G A T C G
NdeI  (3217)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BsgI  (3188)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SbfI  (3154)
1 site		 C C T G C A G G G G A C G T C C
BfuAI  (3143)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3143)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
XcmI  (3005)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
EcoRV  (2944)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NcoI  (2923)
1 site		 C C A T G G G G T A C C
BsmI  (2772)
1 site		 G A A T G C N C T T A C G N
Sticky ends from different BsmI sites may not be compatible.
ApaI  (2758)
1 site		 G G G C C C C C C G G G
* Blocked by Dcm methylation.
ApaI can be used between 25°C and 37°C.
PspOMI  (2754)
1 site		 G G G C C C C C C G G G
* Blocked by Dcm methylation.
BsmBI  (2506)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (2506)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
SpeI  (5369)
1 site		 A C T A G T T G A T C A
PvuII  (5845)
1 site		 C A G C T G G T C G A C
End  (5886)
0 sites
Start  (1)
0 sites
BstEII  (16)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
XbaI  (106)
1 site		 T C T A G A A G A T C T
BsrGI  (285)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
BciVI  (757)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BspHI  (1274)
1 site		 T C A T G A A G T A C T
AhdI  (1447)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BglI  (1567)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
BsaHI  (1984)
1 site		 G R C G Y C C Y G C R G
BsaHI is typically used at 37°C, but is even more active at 60°C.
2μ ori
3953 .. 4833  =  881 bp
yeast 2μ plasmid origin of replication
2μ ori
3953 .. 4833  =  881 bp
yeast 2μ plasmid origin of replication
AmpR
1374 .. 2234  =  861 bp
287 codons
2 segments
   Segment 2:  
   1374 .. 2165  =  792 bp
   264 codons
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1374 .. 2234  =  861 bp
287 codons
2 segments
   Segment 1:  signal sequence  
   2166 .. 2234  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1374 .. 2234  =  861 bp
287 codons
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
URA3
2330 .. 3133  =  804 bp
267 amino acids  =  29.3 kDa
Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis
yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)
URA3
2330 .. 3133  =  804 bp
267 amino acids  =  29.3 kDa
Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis
yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)
ori
615 .. 1203  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
615 .. 1203  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
4902 .. 5357  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4902 .. 5357  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
GAL1 promoter
5376 .. 5817  =  442 bp
inducible promoter, regulated by Gal4
GAL1 promoter
5376 .. 5817  =  442 bp
inducible promoter, regulated by Gal4
CYC1 terminator
120 .. 367  =  248 bp
transcription terminator for CYC1
CYC1 terminator
120 .. 367  =  248 bp
transcription terminator for CYC1
URA3 promoter
3134 .. 3349  =  216 bp
URA3 promoter
3134 .. 3349  =  216 bp
V5 tag
31 .. 72  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
31 .. 72  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
6xHis
82 .. 99  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
82 .. 99  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
T7 promoter
5849 .. 5867  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
5849 .. 5867  =  19 bp
promoter for bacteriophage T7 RNA polymerase
UAS
5376 .. 5493  =  118 bp
upstream activating sequence mediating Gal4-dependent induction
UAS
5376 .. 5493  =  118 bp
upstream activating sequence mediating Gal4-dependent induction
ORF:  1504 .. 1770  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3988 .. 4281  =  294 bp
ORF:  97 amino acids  =  11.6 kDa
ORF:  236 .. 514  =  279 bp
ORF:  92 amino acids  =  10.6 kDa
ORF:  2622 .. 3098  =  477 bp
ORF:  158 amino acids  =  16.8 kDa
ORF:  1374 .. 1610  =  237 bp
ORF:  78 amino acids  =  8.3 kDa
ORF:  1611 .. 2234  =  624 bp
ORF:  207 amino acids  =  23.2 kDa
ORF:  2330 .. 3133  =  804 bp
ORF:  267 amino acids  =  29.3 kDa
ORF:  3734 .. 3973  =  240 bp
ORF:  79 amino acids  =  9.8 kDa
Click here to try SnapGene

Download pYES2.1 V5-His-TOPO (linearized).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

pBAD-TOPO (linearized)
pcDNA5 FRT TO-TOPO (linearized)
pEF6 V5-His-TOPO (linearized)
pGlow-TOPO (linearized)
pBAD Thio-TOPO (linearized)
pcDNA5 FRT V5-His-TOPO (linearized)
pENTR D-TOPO
pIB V5-His-TOPO (linearized)
pBAD202 D-TOPO
pcDNA6.2 C-EmGFP-GW TOPO (linearized)
pENTR SD D-TOPO
pLenti6.3 V5-TOPO (linearized)
pBlue-TOPO (linearized)
pcDNA6.2 C-YFP-GW TOPO (linearized)
pENTR TEV D-TOPO
pLenti6 V5-D-TOPO
pcDNA3.1D V5-His-TOPO
pcDNA6.2 N-EmGFP-GW TOPO (linearized)
pENTR5'-TOPO (linearized)
pLenti7.3 V5-TOPO (linearized)
pcDNA3.2 V5 GW D-TOPO
pCR-XL-2-TOPO (linearized)
pET100 D-TOPO
pLPS-B (linearized)
pcDNA6.2 cGeneBLAzer-GW D-TOPO
pCR-XL-TOPO (linearized)
pET101 D-TOPO
pMT V5-His-TOPO (linearized)
pcDNA6.2 V5 GW D-TOPO
pCR2.1-TOPO (linearized)
pET102 D-TOPO
pOptiVEC-TOPO (linearized)
pcDNA3.1 CT-GFP-TOPO (linearized)
pCR4-TOPO (linearized)
pET151 D-TOPO
pSecTag FRT V5-His-TOPO (linearized)
pcDNA3.1 NT-GFP-TOPO (linearized)
pCR4Blunt-TOPO (linearized)
pET160 GW D-TOPO
pTOP Blunt V2 (linearized)
pcDNA3.1 V5-His-TOPO (linearized)
pCR8 GW TOPO (linearized)
pET161 GW D-TOPO
pTrcHis-TOPO (linearized)
pcDNA3.3-TOPO (linearized)
pCR-Blunt II-TOPO (linearized)
pET200 D-TOPO
pTrcHis2-TOPO (linearized)
pcDNA3.4-TOPO (linearized)
pCRII-TOPO (linearized)
pEXP5-CT TOPO (linearized)
pYES2.1 V5-His-TOPO (linearized)
pcDNA4 HisMax-TOPO (linearized)
pEF5 FRT V5-D-TOPO
pEXP5-NT TOPO (linearized)

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free