pMT V5-His-TOPO (linearized)

Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products and inducible expression in Drosophila cells.

Sequence Author: Thermo Fisher (Invitrogen)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: TOPO Cloning Vectors | More Plasmid Sets
No matches
HomePlasmidsTOPO Cloning VectorspMT V5-His-TOPO (linearized)
AatII (2611) ZraI (2609) SspI (2493) ScaI (2169) NmeAIII (1837) BpmI (1759) AhdI (1689) AlwNI (1212) PspFI (1104) BseYI (1100) PfoI (2721) BstAPI (2860) KasI (2910) NarI (2911) SfoI (2912) PluTI (2914) BsgI (3315) BmgBI (3337) MscI (3446) Acc65I (3524) KpnI (3528) SpeI (3531) End (3558) Start (1) EcoRV (18) NotI (33) AvaI - BsoBI - PaeR7I - PspXI - XhoI (39) PspOMI (51) ApaI - BanII - BtgI (55) SacII (58) BstBI (62) BseRI (79) MluI (106) AgeI (112) 6xHis PmeI (142) PsiI (291) HpaI (311) MfeI (320) SalI (419) AccI (420) SbfI (429) BfuAI - BspMI (432) BspQI - SapI (680) PciI (796) pMT/V5-His-TOPO® 3557 bp
AatII  (2611)
1 site		 G A C G T C C T G C A G
ZraI  (2609)
1 site		 G A C G T C C T G C A G
SspI  (2493)
1 site		 A A T A T T T T A T A A
ScaI  (2169)
1 site		 A G T A C T T C A T G A
NmeAIII  (1837)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (1759)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
AhdI  (1689)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (1212)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
PspFI  (1104)
1 site		 C C C A G C G G G T C G
BseYI  (1100)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PfoI  (2721)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
BstAPI  (2860)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
KasI  (2910)
1 site		 G G C G C C C C G C G G
NarI  (2911)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (2912)
1 site		 G G C G C C C C G C G G
PluTI  (2914)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BsgI  (3315)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BmgBI  (3337)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
MscI  (3446)
1 site		 T G G C C A A C C G G T
Acc65I  (3524)
1 site		 G G T A C C C C A T G G
KpnI  (3528)
1 site		 G G T A C C C C A T G G
SpeI  (3531)
1 site		 A C T A G T T G A T C A
End  (3558)
0 sites
Start  (1)
0 sites
EcoRV  (18)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NotI  (33)
1 site		 G C G G C C G C C G C C G G C G
AvaI  (39)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (39)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (39)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (39)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (39)
1 site		 C T C G A G G A G C T C
PspOMI  (51)
1 site		 G G G C C C C C C G G G
ApaI  (55)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BanII  (55)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
BtgI  (55)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
SacII  (58)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstBI  (62)
1 site		 T T C G A A A A G C T T
BseRI  (79)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
MluI  (106)
1 site		 A C G C G T T G C G C A
AgeI  (112)
1 site		 A C C G G T T G G C C A
PmeI  (142)
1 site		 G T T T A A A C C A A A T T T G
PsiI  (291)
1 site		 T T A T A A A A T A T T
HpaI  (311)
1 site		 G T T A A C C A A T T G
MfeI  (320)
1 site		 C A A T T G G T T A A C
SalI  (419)
1 site		 G T C G A C C A G C T G
AccI  (420)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SbfI  (429)
1 site		 C C T G C A G G G G A C G T C C
BfuAI  (432)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (432)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BspQI  (680)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (680)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (796)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
AmpR
1616 .. 2476  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1616 .. 2407  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1616 .. 2476  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2408 .. 2476  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1616 .. 2476  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
857 .. 1445  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ori
857 .. 1445  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
MT promoter
3082 .. 3508  =  427 bp
Drosophila metallothionein promoter
MT promoter
3082 .. 3508  =  427 bp
Drosophila metallothionein promoter
SV40 poly(A) signal
177 .. 311  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
177 .. 311  =  135 bp
SV40 polyadenylation signal
AmpR promoter
2477 .. 2581  =  105 bp
AmpR promoter
2477 .. 2581  =  105 bp
V5 tag
67 .. 108  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
67 .. 108  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
6xHis
118 .. 135  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
118 .. 135  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
ORF:  3238 .. 3486  =  249 bp
ORF:  82 amino acids  =  9.2 kDa
ORF:  2861 .. 3121  =  261 bp
ORF:  86 amino acids  =  9.9 kDa
ORF:  1746 .. 2012  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3141 .. 3557  =  417 bp
ORF:  138 amino acids  =  15.8 kDa  (no start codon)
ORF:  1616 .. 2476  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3080 .. 3457  =  378 bp
ORF:  125 amino acids  =  13.3 kDa
Click here to try SnapGene

Download pMT V5-His-TOPO (linearized).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

pBAD-TOPO (linearized)
pcDNA5 FRT TO-TOPO (linearized)
pEF6 V5-His-TOPO (linearized)
pGlow-TOPO (linearized)
pBAD Thio-TOPO (linearized)
pcDNA5 FRT V5-His-TOPO (linearized)
pENTR D-TOPO
pIB V5-His-TOPO (linearized)
pBAD202 D-TOPO
pcDNA6.2 C-EmGFP-GW TOPO (linearized)
pENTR SD D-TOPO
pLenti6.3 V5-TOPO (linearized)
pBlue-TOPO (linearized)
pcDNA6.2 C-YFP-GW TOPO (linearized)
pENTR TEV D-TOPO
pLenti6 V5-D-TOPO
pcDNA3.1D V5-His-TOPO
pcDNA6.2 N-EmGFP-GW TOPO (linearized)
pENTR5'-TOPO (linearized)
pLenti7.3 V5-TOPO (linearized)
pcDNA3.2 V5 GW D-TOPO
pCR-XL-2-TOPO (linearized)
pET100 D-TOPO
pLPS-B (linearized)
pcDNA6.2 cGeneBLAzer-GW D-TOPO
pCR-XL-TOPO (linearized)
pET101 D-TOPO
pMT V5-His-TOPO (linearized)
pcDNA6.2 V5 GW D-TOPO
pCR2.1-TOPO (linearized)
pET102 D-TOPO
pOptiVEC-TOPO (linearized)
pcDNA3.1 CT-GFP-TOPO (linearized)
pCR4-TOPO (linearized)
pET151 D-TOPO
pSecTag FRT V5-His-TOPO (linearized)
pcDNA3.1 NT-GFP-TOPO (linearized)
pCR4Blunt-TOPO (linearized)
pET160 GW D-TOPO
pTOP Blunt V2 (linearized)
pcDNA3.1 V5-His-TOPO (linearized)
pCR8 GW TOPO (linearized)
pET161 GW D-TOPO
pTrcHis-TOPO (linearized)
pcDNA3.3-TOPO (linearized)
pCR-Blunt II-TOPO (linearized)
pET200 D-TOPO
pTrcHis2-TOPO (linearized)
pcDNA3.4-TOPO (linearized)
pCRII-TOPO (linearized)
pEXP5-CT TOPO (linearized)
pYES2.1 V5-His-TOPO (linearized)
pcDNA4 HisMax-TOPO (linearized)
pEF5 FRT V5-D-TOPO
pEXP5-NT TOPO (linearized)

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free