pMCentr1 (linearized)

Linearized vector for ligation-independent cloning (LIC) to create a Gateway® entry vector, with a Zeocin™ resistance marker and a TEV cleavage site.

Sequence Author: Midwest Center for Structural Genomics

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HomePlasmidsStructural Genomics VectorspMCentr1 (linearized)
PciI (1415) DrdI (1313) BssSI - BssSαI (1242) BciVI (1217) AlwNI (1006) AcuI - Eco57MI (873) BsaAI - PmlI (683) NcoI - StyI (612) MscI (611) BssHII - MauBI (574) AatII (572) ZraI (570) SmaI (524) TspMI - XmaI (522) SgrAI (496) BmgBI (480) CsiI - SexAI * (446) BanI (440) FseI (342) BglI (312) NspI (1419) BspQI - SapI (1532) BtsI - BtsαI (1635) TsoI (1678) AclI (1785) BbsI (1858) HpaI (1922) AhdI (1970) AflII (1975) PspOMI (1984) EcoO109I (1985) ApaI - BanII (1988) MslI (2039) End (2100) Start (0) EcoRV (129) BseRI (241) DraIII (264) EagI (279) pMCentr1 2100 bp
PciI  (1415)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
DrdI  (1313)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
BssSI  (1242)
1 site		 C A C G A G G T G C T C
BssSαI  (1242)
1 site		 C A C G A G G T G C T C
BciVI  (1217)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
AlwNI  (1006)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
AcuI  (873)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (873)
1 site		 C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14
Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaAI  (683)
1 site		 Y A C G T R R T G C A Y
PmlI  (683)
1 site		 C A C G T G G T G C A C
NcoI  (612)
1 site		 C C A T G G G G T A C C
StyI  (612)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
MscI  (611)
1 site		 T G G C C A A C C G G T
BssHII  (574)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
MauBI  (574)
1 site		 C G C G C G C G G C G C G C G C
AatII  (572)
1 site		 G A C G T C C T G C A G
ZraI  (570)
1 site		 G A C G T C C T G C A G
SmaI  (524)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
TspMI  (522)
1 site		 C C C G G G G G G C C C
XmaI  (522)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SgrAI  (496)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BmgBI  (480)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
CsiI  (446)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (446)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BanI  (440)
1 site		 G G Y R C C C C R Y G G
Sticky ends from different BanI sites may not be compatible.
FseI  (342)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
BglI  (312)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
NspI  (1419)
1 site		 R C A T G Y Y G T A C R
BspQI  (1532)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (1532)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BtsI  (1635)
1 site		 G C A G T G N N C G T C A C
BtsαI  (1635)
1 site		 G C A G T G N N C G T C A C
Sticky ends from different BtsαI sites may not be compatible.
TsoI  (1678)
1 site		 T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9
Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AclI  (1785)
1 site		 A A C G T T T T G C A A
BbsI  (1858)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
HpaI  (1922)
1 site		 G T T A A C C A A T T G
AhdI  (1970)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AflII  (1975)
1 site		 C T T A A G G A A T T C
PspOMI  (1984)
1 site		 G G G C C C C C C G G G
EcoO109I  (1985)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
ApaI  (1988)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BanII  (1988)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
MslI  (2039)
1 site		 C A Y N N N N R T G G T R N N N N Y A C
End  (2100)
0 sites
Start  (0)
0 sites
EcoRV  (129)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BseRI  (241)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
DraIII  (264)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
EagI  (279)
1 site		 C G G C C G G C C G G C
ori
771 .. 1359  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
771 .. 1359  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
BleoR
241 .. 615  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
241 .. 615  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
attL2
17 .. 116  =  100 bp
recombination site for the Gateway® LR reaction
attL2
17 .. 116  =  100 bp
recombination site for the Gateway® LR reaction
attL1
1990 .. 2089  =  100 bp
recombination site for the Gateway® LR reaction
attL1
1990 .. 2089  =  100 bp
recombination site for the Gateway® LR reaction
rrnB T1 terminator
1808 .. 1894  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
1808 .. 1894  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
EM7 promoter
634 .. 681  =  48 bp
synthetic bacterial promoter
EM7 promoter
634 .. 681  =  48 bp
synthetic bacterial promoter
rrnB T2 terminator
1689 .. 1716  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
1689 .. 1716  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
TEV site
2092 .. 2112  =  21 bp
7 amino acids  =  900.0 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV site
2092 .. 2112  =  21 bp
7 amino acids  =  900.0 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
T7 promoter
134 .. 152  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
134 .. 152  =  19 bp
promoter for bacteriophage T7 RNA polymerase
M13 rev
157 .. 173  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
157 .. 173  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
1958 .. 1974  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
1958 .. 1974  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  335 .. 622  =  288 bp
ORF:  95 amino acids  =  10.1 kDa
ORF:  237 .. 467  =  231 bp
ORF:  76 amino acids  =  8.2 kDa
ORF:  241 .. 615  =  375 bp
ORF:  124 amino acids  =  13.8 kDa
ORF:  1815 .. 2114  =  300 bp
ORF:  99 amino acids  =  11.3 kDa  (no start codon)
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Individual Sequences & Maps

p11
pMCSG19B (linearized)
pMCSG41
pMCSG68 (linearized)
p15Tv-L
pMCSG19C
pMCSG41 (linearized)
pMCSG69
p283
pMCSG19C (linearized)
pMCSG42
pMCSG69 (linearized)
pBEN1-SGC
pMCSG20
pMCSG42 (linearized)
pMCSG7
pBEN1-SGC (linearized)
pMCSG20 (linearized)
pMCSG43
pMCSG7 (linearized)
pDB-HisGST
pMCSG21
pMCSG43 (linearized)
pMCSG70
pDB.GST
pMCSG21 (linearized)
pMCSG46
pMCSG70 (linearized)
pDB.His.MBP
pMCSG22
pMCSG46 (linearized)
pMCSG71
pDB.His.NusA
pMCSG22 (linearized)
pMCSG48
pMCSG71 (linearized)
pDB.His.TRX
pMCSG23
pMCSG48 (linearized)
pMCSG73
pDB.TRX
pMCSG23 (linearized)
pMCSG49
pMCSG73 (linearized)
pEU-GST-FV
pMCSG24
pMCSG49 (linearized)
pMCSG75
pEU-His-FV
pMCSG24 (linearized)
pMCSG50
pMCSG75 (linearized)
pEU-HSBC
pMCSG25
pMCSG50 (linearized)
pMCSG76
pFB-LIC-Bse
pMCSG25 (linearized)
pMCSG51
pMCSG76 (linearized)
pFB-LIC-Bse (linearized)
pMCSG26
pMCSG51 (linearized)
pMCSG77
pH3c-LIC
pMCSG26 (linearized)
pMCSG52
pMCSG77 (linearized)
pH3c-LIC (linearized)
pMCSG27
pMCSG52 (linearized)
pMCSG79
pHM3C-LIC
pMCSG27 (linearized)
pMCSG53
pMCSG79 (linearized)
pHM3C-LIC (linearized)
pMCSG28
pMCSG53 (linearized)
pMCSG8
pLIC-CH
pMCSG28 (linearized)
pMCSG54
pMCSG8 (linearized)
pLIC-CH (linearized)
pMCSG29
pMCSG54 (linearized)
pMCSG81
pLIC-EGFP
pMCSG29 (linearized)
pMCSG55
pMCSG81 (linearized)
pLIC-EGFP (linearized)
pMCSG30
pMCSG55 (linearized)
pMCSG9
pLIC-SGC1
pMCSG30 (linearized)
pMCSG56
pMCSG9 (linearized)
pLIC-SGC1 (linearized)
pMCSG31
pMCSG56 (linearized)
pNH-TrxT
pMCentr1
pMCSG31 (linearized)
pMCSG57
pNH-TrxT (linearized)
pMCentr1 (linearized)
pMCSG32
pMCSG57 (linearized)
pNIC-CH
pMCentr2
pMCSG32 (linearized)
pMCSG58
pNIC-CH (linearized)
pMCentr2 (linearized)
pMCSG33
pMCSG58 (linearized)
pNIC-CTHF
pMCentr3
pMCSG33 (linearized)
pMCSG59
pNIC-CTHF (linearized)
pMCentr3 (linearized)
pMCSG34
pMCSG59 (linearized)
pNIC-Zb
pMCSG10
pMCSG34 (linearized)
pMCSG60
pNIC-Zb (linearized)
pMCSG10 (linearized)
pMCSG34B
pMCSG60 (linearized)
pNIC28-Bsa4
pMCSG11
pMCSG34B (linearized)
pMCSG61
pNIC28-Bsa4 (linearized)
pMCSG11 (linearized)
pMCSG35
pMCSG61 (linearized)
pNYCOMPS-LIC-FH10T+ (N term) - linearized
pMCSG12
pMCSG35 (linearized)
pMCSG62
pNYCOMPS-LIC-FH10T+ (N term)
pMCSG12 (linearized)
pMCSG35B
pMCSG62 (linearized)
pSpeedET
pMCSG13
pMCSG35B (linearized)
pMCSG63
pVP13
pMCSG13 (linearized)
pMCSG37
pMCSG63 (linearized)
pVP16
pMCSG14
pMCSG37 (linearized)
pMCSG64
pVP33A
pMCSG14 (linearized)
pMCSG38
pMCSG64 (linearized)
pVP33K
pMCSG17
pMCSG38 (linearized)
pMCSG65
pVP56A
pMCSG17 (linearized)
pMCSG38C
pMCSG65 (linearized)
pVP56K
pMCSG18
pMCSG38C (linearized)
pMCSG66
pVP65K
pMCSG18 (linearized)
pMCSG39
pMCSG66 (linearized)
pVP68K
pMCSG19
pMCSG39 (linearized)
pMCSG67
pVP81K
pMCSG19 (linearized)
pMCSG40
pMCSG67 (linearized)
pVP91A
pMCSG19B
pMCSG40 (linearized)
pMCSG68

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Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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