pVP56K

Bacterial Flexi® Vector with a kanamycin resistance marker, for appending an N-terminal 8xHis-MBP-HRV 3C-TEV cassette to an expressed protein.

Sequence Author: Center for Eukaryotic Structural Genomics

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Structural Genomics Vectors | More Plasmid Sets
No matches
HomePlasmidsStructural Genomics VectorspVP56K
PaeR7I - XhoI (117) NaeI (6448) NgoMIV (6446) RsrII (6431) AvrII (6279) PciI (5459) NdeI (5282) BstZ17I (5232) FspAI (5039) PpuMI * (5022) BstEII (4387) EcoRV (4123) HpaI (4067) AanI (165) MfeI (175) lac operator RBS BseRI (231) ATG NsiI (263) BsaI (327) AanI (616) BmgBI (806) AfeI (1174) PacI (1369) TEV site AsiSI - SgfI (1435) NotI (1438) barnase PmeI (2567) Eco53kI (2580) SacI (2582) SbfI (2615) BsrGI (2721) AscI (2738) SgrDI (2753) BspDI - ClaI (2766) BstBI (2777) HindIII (2780) NheI (2900) BmtI (2904) pVP56K 7253 bp
PaeR7I  (117)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (117)
1 site		 C T C G A G G A G C T C
NaeI  (6448)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (6446)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
RsrII  (6431)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AvrII  (6279)
1 site		 C C T A G G G G A T C C
PciI  (5459)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
NdeI  (5282)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstZ17I  (5232)
1 site		 G T A T A C C A T A T G
FspAI  (5039)
1 site		 R T G C G C A Y Y A C G C G T R
PpuMI  (5022)
1 site		 R G G W C C Y Y C C W G G R
* Blocked by Dcm methylation.
Sticky ends from different PpuMI sites may not be compatible.
BstEII  (4387)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
EcoRV  (4123)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
HpaI  (4067)
1 site		 G T T A A C C A A T T G
AanI  (165)
2 sites		 T T A T A A A A T A T T
MfeI  (175)
1 site		 C A A T T G G T T A A C
BseRI  (231)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
NsiI  (263)
1 site		 A T G C A T T A C G T A
BsaI  (327)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AanI  (616)
2 sites		 T T A T A A A A T A T T
BmgBI  (806)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
AfeI  (1174)
1 site		 A G C G C T T C G C G A
PacI  (1369)
1 site		 T T A A T T A A A A T T A A T T
AsiSI  (1435)
1 site		 G C G A T C G C C G C T A G C G
SgfI  (1435)
1 site		 G C G A T C G C C G C T A G C G
NotI  (1438)
1 site		 G C G G C C G C C G C C G G C G
PmeI  (2567)
1 site		 G T T T A A A C C A A A T T T G
Eco53kI  (2580)
1 site		 G A G C T C C T C G A G
SacI  (2582)
1 site		 G A G C T C C T C G A G
SbfI  (2615)
1 site		 C C T G C A G G G G A C G T C C
BsrGI  (2721)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
AscI  (2738)
1 site		 G G C G C G C C C C G C G C G G
SgrDI  (2753)
1 site		 C G T C G A C G G C A G C T G C
BspDI  (2766)
1 site		 A T C G A T T A G C T A
ClaI  (2766)
1 site		 A T C G A T T A G C T A
BstBI  (2777)
1 site		 T T C G A A A A G C T T
HindIII  (2780)
1 site		 A A G C T T T T C G A A
NheI  (2900)
1 site		 G C T A G C C G A T C G
BmtI  (2904)
1 site		 G C T A G C C G A T C G
ATG
231 .. 233  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
231 .. 233  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
8xHis
237 .. 260  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
8xHis
237 .. 260  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
MBP
267 .. 1364  =  1098 bp
366 amino acids  =  40.2 kDa
Product: maltose binding protein from E. coli
This version of the gene does not encode a signal sequence, so MBP will remain in the cytosol.
MBP
267 .. 1364  =  1098 bp
366 amino acids  =  40.2 kDa
Product: maltose binding protein from E. coli
This version of the gene does not encode a signal sequence, so MBP will remain in the cytosol.
HRV 3C site
1389 .. 1412  =  24 bp
8 amino acids  =  902.1 Da
Product: recognition and cleavage site for human rhinovirus 3C and PreScission proteases
HRV 3C site
1389 .. 1412  =  24 bp
8 amino acids  =  902.1 Da
Product: recognition and cleavage site for human rhinovirus 3C and PreScission proteases
TEV site
1413 .. 1433  =  21 bp
7 amino acids  =  884.0 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV site
1413 .. 1433  =  21 bp
7 amino acids  =  884.0 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
lacI
3842 .. 4924  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
3842 .. 4924  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
NeoR/KanR
6285 .. 7079  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
6285 .. 7079  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
CmR
1903 .. 2562  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
1903 .. 2562  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
2939 .. 3598  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
2939 .. 3598  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
5520 .. 6108  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5520 .. 6108  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
barnase
1468 .. 1803  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
barnase
1468 .. 1803  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
lambda t0 terminator
2801 .. 2895  =  95 bp
transcription terminator from phage lambda
lambda t0 terminator
2801 .. 2895  =  95 bp
transcription terminator from phage lambda
rrnB T1 terminator
3663 .. 3749  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
3663 .. 3749  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
lacIq promoter
4925 .. 5002  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
lacIq promoter
4925 .. 5002  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
T5 promoter
126 .. 170  =  45 bp
4 segments
   Segment 1:  
   126 .. 140  =  15 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
126 .. 170  =  45 bp
4 segments
   Segment 2:  -35  
   141 .. 146  =  6 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
126 .. 170  =  45 bp
4 segments
   Segment 3:  
   147 .. 163  =  17 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
126 .. 170  =  45 bp
4 segments
   Segment 4:  -10  
   164 .. 170  =  7 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
126 .. 170  =  45 bp
4 segments
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
lac UV5 promoter
1819 .. 1849  =  31 bp
3 segments
   Segment 1:  -35  
   1819 .. 1824  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1819 .. 1849  =  31 bp
3 segments
   Segment 2:  
   1825 .. 1842  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1819 .. 1849  =  31 bp
3 segments
   Segment 3:  -10  
   1843 .. 1849  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1819 .. 1849  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
lac operator
178 .. 194  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
178 .. 194  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RBS
217 .. 222  =  6 bp
ribosome binding site
RBS
217 .. 222  =  6 bp
ribosome binding site
lac operator
146 .. 162  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
146 .. 162  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
ORF:  1468 .. 1803  =  336 bp
ORF:  111 amino acids  =  12.5 kDa
ORF:  1903 .. 2562  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  3808 .. 4059  =  252 bp
ORF:  83 amino acids  =  9.1 kDa
ORF:  2939 .. 3598  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  231 .. 1454  =  1224 bp
ORF:  407 amino acids  =  44.7 kDa
ORF:  6516 .. 6770  =  255 bp
ORF:  84 amino acids  =  9.8 kDa
ORF:  3822 .. 4085  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  4674 .. 4991  =  318 bp
ORF:  105 amino acids  =  11.2 kDa
ORF:  6285 .. 7079  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  3842 .. 4801  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
ORF:  6521 .. 6907  =  387 bp
ORF:  128 amino acids  =  14.7 kDa
Click here to try SnapGene

Download pVP56K.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

p11
pMCSG19B (linearized)
pMCSG41
pMCSG68 (linearized)
p15Tv-L
pMCSG19C
pMCSG41 (linearized)
pMCSG69
p283
pMCSG19C (linearized)
pMCSG42
pMCSG69 (linearized)
pBEN1-SGC
pMCSG20
pMCSG42 (linearized)
pMCSG7
pBEN1-SGC (linearized)
pMCSG20 (linearized)
pMCSG43
pMCSG7 (linearized)
pDB-HisGST
pMCSG21
pMCSG43 (linearized)
pMCSG70
pDB.GST
pMCSG21 (linearized)
pMCSG46
pMCSG70 (linearized)
pDB.His.MBP
pMCSG22
pMCSG46 (linearized)
pMCSG71
pDB.His.NusA
pMCSG22 (linearized)
pMCSG48
pMCSG71 (linearized)
pDB.His.TRX
pMCSG23
pMCSG48 (linearized)
pMCSG73
pDB.TRX
pMCSG23 (linearized)
pMCSG49
pMCSG73 (linearized)
pEU-GST-FV
pMCSG24
pMCSG49 (linearized)
pMCSG75
pEU-His-FV
pMCSG24 (linearized)
pMCSG50
pMCSG75 (linearized)
pEU-HSBC
pMCSG25
pMCSG50 (linearized)
pMCSG76
pFB-LIC-Bse
pMCSG25 (linearized)
pMCSG51
pMCSG76 (linearized)
pFB-LIC-Bse (linearized)
pMCSG26
pMCSG51 (linearized)
pMCSG77
pH3c-LIC
pMCSG26 (linearized)
pMCSG52
pMCSG77 (linearized)
pH3c-LIC (linearized)
pMCSG27
pMCSG52 (linearized)
pMCSG79
pHM3C-LIC
pMCSG27 (linearized)
pMCSG53
pMCSG79 (linearized)
pHM3C-LIC (linearized)
pMCSG28
pMCSG53 (linearized)
pMCSG8
pLIC-CH
pMCSG28 (linearized)
pMCSG54
pMCSG8 (linearized)
pLIC-CH (linearized)
pMCSG29
pMCSG54 (linearized)
pMCSG81
pLIC-EGFP
pMCSG29 (linearized)
pMCSG55
pMCSG81 (linearized)
pLIC-EGFP (linearized)
pMCSG30
pMCSG55 (linearized)
pMCSG9
pLIC-SGC1
pMCSG30 (linearized)
pMCSG56
pMCSG9 (linearized)
pLIC-SGC1 (linearized)
pMCSG31
pMCSG56 (linearized)
pNH-TrxT
pMCentr1
pMCSG31 (linearized)
pMCSG57
pNH-TrxT (linearized)
pMCentr1 (linearized)
pMCSG32
pMCSG57 (linearized)
pNIC-CH
pMCentr2
pMCSG32 (linearized)
pMCSG58
pNIC-CH (linearized)
pMCentr2 (linearized)
pMCSG33
pMCSG58 (linearized)
pNIC-CTHF
pMCentr3
pMCSG33 (linearized)
pMCSG59
pNIC-CTHF (linearized)
pMCentr3 (linearized)
pMCSG34
pMCSG59 (linearized)
pNIC-Zb
pMCSG10
pMCSG34 (linearized)
pMCSG60
pNIC-Zb (linearized)
pMCSG10 (linearized)
pMCSG34B
pMCSG60 (linearized)
pNIC28-Bsa4
pMCSG11
pMCSG34B (linearized)
pMCSG61
pNIC28-Bsa4 (linearized)
pMCSG11 (linearized)
pMCSG35
pMCSG61 (linearized)
pNYCOMPS-LIC-FH10T+ (N term) - linearized
pMCSG12
pMCSG35 (linearized)
pMCSG62
pNYCOMPS-LIC-FH10T+ (N term)
pMCSG12 (linearized)
pMCSG35B
pMCSG62 (linearized)
pSpeedET
pMCSG13
pMCSG35B (linearized)
pMCSG63
pVP13
pMCSG13 (linearized)
pMCSG37
pMCSG63 (linearized)
pVP16
pMCSG14
pMCSG37 (linearized)
pMCSG64
pVP33A
pMCSG14 (linearized)
pMCSG38
pMCSG64 (linearized)
pVP33K
pMCSG17
pMCSG38 (linearized)
pMCSG65
pVP56A
pMCSG17 (linearized)
pMCSG38C
pMCSG65 (linearized)
pVP56K
pMCSG18
pMCSG38C (linearized)
pMCSG66
pVP65K
pMCSG18 (linearized)
pMCSG39
pMCSG66 (linearized)
pVP68K
pMCSG19
pMCSG39 (linearized)
pMCSG67
pVP81K
pMCSG19 (linearized)
pMCSG40
pMCSG67 (linearized)
pVP91A
pMCSG19B
pMCSG40 (linearized)
pMCSG68

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free