Allele TA Vector (linearized)

Linearized vector with 3'-T overhangs and multiple flanking restriction sites for TA cloning of PCR products.

Sequence Author: Allele Biotechnology

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HomePlasmidsTA and GC Cloning VectorsAllele TA Vector (linearized)
EcoO109I (2260) AatII (2206) ZraI (2204) SspI (2088) XmnI (1883) ScaI (1764) TsoI (1683) NmeAIII (1432) BpmI (1354) BsaI (1345) AhdI (1284) AlwNI (807) PfoI (2317) NdeI (2455) BstAPI (2456) KasI (2506) NarI (2507) SfoI (2508) PluTI (2510) M13 fwd ApoI - EcoRI (2667) Eco53kI (2675) BanII - SacI (2677) AvaI - BsoBI - TspMI - XmaI (2683) SmaI (2685) BamHI (2688) XbaI * (2694) End (2708) Start (1) AgeI (10) SalI (14) AccI (15) HincII (16) PstI - SbfI (24) BfuAI - BspMI (27) SphI (30) HindIII (32) M13 rev lac operator lac promoter BspQI - SapI (275) AflIII - PciI (391) BseYI (695) PspFI (699) Allele TA Vector 2707 bp
EcoO109I  (2260)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
AatII  (2206)
1 site		 G A C G T C C T G C A G
ZraI  (2204)
1 site		 G A C G T C C T G C A G
SspI  (2088)
1 site		 A A T A T T T T A T A A
XmnI  (1883)
1 site		 G A A N N N N T T C C T T N N N N A A G
ScaI  (1764)
1 site		 A G T A C T T C A T G A
TsoI  (1683)
1 site		 T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9
Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
NmeAIII  (1432)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (1354)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsaI  (1345)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (1284)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (807)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
PfoI  (2317)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
NdeI  (2455)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstAPI  (2456)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
KasI  (2506)
1 site		 G G C G C C C C G C G G
NarI  (2507)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (2508)
1 site		 G G C G C C C C G C G G
PluTI  (2510)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
ApoI  (2667)
1 site		 R A A T T Y Y T T A A R
ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (2667)
1 site		 G A A T T C C T T A A G
Eco53kI  (2675)
1 site		 G A G C T C C T C G A G
BanII  (2677)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
SacI  (2677)
1 site		 G A G C T C C T C G A G
AvaI  (2683)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (2683)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI  (2683)
1 site		 C C C G G G G G G C C C
XmaI  (2683)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2685)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BamHI  (2688)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (2694)
1 site		 T C T A G A A G A T C T
* Blocked by Dam methylation.
End  (2708)
0 sites
Start  (1)
0 sites
AgeI  (10)
1 site		 A C C G G T T G G C C A
SalI  (14)
1 site		 G T C G A C C A G C T G
AccI  (15)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (16)
1 site		 G T Y R A C C A R Y T G
PstI  (24)
1 site		 C T G C A G G A C G T C
SbfI  (24)
1 site		 C C T G C A G G G G A C G T C C
BfuAI  (27)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (27)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SphI  (30)
1 site		 G C A T G C C G T A C G
HindIII  (32)
1 site		 A A G C T T T T C G A A
BspQI  (275)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (275)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AflIII  (391)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (391)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BseYI  (695)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PspFI  (699)
1 site		 C C C A G C G G G T C G
AmpR
1211 .. 2071  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1211 .. 2002  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1211 .. 2071  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2003 .. 2071  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1211 .. 2071  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
452 .. 1040  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
452 .. 1040  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
AmpR promoter
2072 .. 2176  =  105 bp
AmpR promoter
2072 .. 2176  =  105 bp
MCS
2667 .. 2707  =  41 bp
multiple cloning site
MCS
2667 .. 2707  =  41 bp
multiple cloning site
MCS
2 .. 37  =  36 bp
multiple cloning site
MCS
2 .. 37  =  36 bp
multiple cloning site
lac promoter
98 .. 128  =  31 bp
3 segments
   Segment 3:  -10  
   98 .. 104  =  7 bp
promoter for the E. coli lac operon
lac promoter
98 .. 128  =  31 bp
3 segments
   Segment 2:  
   105 .. 122  =  18 bp
promoter for the E. coli lac operon
lac promoter
98 .. 128  =  31 bp
3 segments
   Segment 1:  -35  
   123 .. 128  =  6 bp
promoter for the E. coli lac operon
lac promoter
98 .. 128  =  31 bp
3 segments
promoter for the E. coli lac operon
M13 rev
50 .. 66  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
50 .. 66  =  17 bp
common sequencing primer, one of multiple similar variants
lac operator
74 .. 90  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
74 .. 90  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
M13 fwd
2650 .. 2666  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
2650 .. 2666  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  1341 .. 1607  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2457 .. 2708  =  252 bp
ORF:  84 amino acids  =  9.8 kDa
ORF:  1211 .. 2071  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2417 .. 2707  =  291 bp
ORF:  96 amino acids  =  11.0 kDa  (no start codon)
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Individual Sequences & Maps

Allele TA Vector (linearized)
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