pBAD Thio-TOPO (linearized)

Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning and arabinose-inducible expression of a His-Patch thioredoxin fusion protein.

Sequence Author: Thermo Fisher (Invitrogen)

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HomePlasmidsTA and GC Cloning VectorspBAD Thio-TOPO (linearized)
End (4454) Start (1) pBAD/Thio-TOPO® 4453 bp
End  (4454)
0 sites
Start  (1)
0 sites
araC
2836 .. 3714  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
araC
2836 .. 3714  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
AmpR
627 .. 1487  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   627 .. 695  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
627 .. 1487  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   696 .. 1487  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
627 .. 1487  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1658 .. 2246  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ori
1658 .. 2246  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
HP-thioredoxin
4086 .. 4415  =  330 bp
110 amino acids  =  11.9 kDa
Product: His-Patch thioredoxin
The histidines at positions 8, 32, and 64 coordinate a divalent cation, allowing HP-thioredoxin to be purified on metal-chelating resins.
HP-thioredoxin
4086 .. 4415  =  330 bp
110 amino acids  =  11.9 kDa
Product: His-Patch thioredoxin
The histidines at positions 8, 32, and 64 coordinate a divalent cation, allowing HP-thioredoxin to be purified on metal-chelating resins.
enterokinase site
4431 .. 4445  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
4431 .. 4445  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
araBAD promoter
3860 .. 4025  =  166 bp
promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction
araBAD promoter
3860 .. 4025  =  166 bp
promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction
AmpR promoter
535 .. 626  =  92 bp
AmpR promoter
535 .. 626  =  92 bp
rrnB T1 terminator
310 .. 396  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
310 .. 396  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
V5 tag
16 .. 57  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
16 .. 57  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
6xHis
67 .. 84  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
67 .. 84  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
rrnB T2 terminator
488 .. 515  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
488 .. 515  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
RBS
4069 .. 4074  =  6 bp
ribosome binding site
RBS
4069 .. 4074  =  6 bp
ribosome binding site
ORF:  3400 .. 3648  =  249 bp
ORF:  82 amino acids  =  9.3 kDa
ORF:  506 .. 730  =  225 bp
ORF:  74 amino acids  =  8.7 kDa
ORF:  627 .. 1487  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  4086 .. 4454  =  369 bp
ORF:  123 amino acids  =  13.1 kDa
ORF:  4041 .. 4454  =  414 bp
ORF:  137 amino acids  =  15.9 kDa  (no start codon)
ORF:  1091 .. 1357  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  151 .. 477  =  327 bp
ORF:  108 amino acids  =  12.1 kDa
ORF:  2836 .. 3765  =  930 bp
ORF:  309 amino acids  =  35.2 kDa
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Individual Sequences & Maps

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pcDNA3.1 V5-His-TOPO (linearized)
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pLUG-Multi (linearized)
pTOP TA V2 (linearized)
pcDNA3.3-TOPO (linearized)
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pTZ57R_T
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pcDNA5 FRT V5-His-TOPO (linearized)
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pGEM-T Easy (linearized)
pSMART GC HK (linearized)
T-Vector pMD19 (Simple)
pcDNA6.2 N-EmGFP-GW TOPO (linearized)
pGlow-TOPO (linearized)
pSMART GC LK (linearized)
T-Vector pMD20
pCR-XL-TOPO (linearized)
pIB V5-His-TOPO (linearized)

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