pJAZZ-OK GC (left arm)

Left arm of a linear bacterial vector with a kanamycin resistance gene, processed for GC cloning of DNA sequences that are unstable in circular plasmids.

Sequence Author: Lucigen

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: TA and GC Cloning Vectors | More Plasmid Sets
No matches
HomePlasmidsTA and GC Cloning VectorspJAZZ-OK GC (left arm)
10,000 7500 5000 2500 End (10,312) NotI - EagI (10,300) SpeI (10,293) SL1 (10,272 .. 10,292) XbaI (10,207) BssSI - BssSαI (9891) RsrII (9656) PshAI (8685) BbvCI (8044) PluTI (7282) SfoI (7280) NarI (7279) KasI (7278) NdeI (7274) XhoI PspXI PaeR7I (6350) BsrGI (5170) AatII (4824) ZraI (4822) BstBI (4086) CsiI - SexAI * (3996) PasI (3256) MauBI BssHII (3125) SacI (1874) Eco53kI (1872) PaqCI (1785) PpuMI (1632) SgrDI (1402) StyI (1288) BstZ17I (1064) PmeI (956) MmeI (762) MfeI (248) Start (0) telL TelN RepA CB T7Te terminator N15 ori pJAZZ®-OK GC (left arm) 10,312 bp
End  (10,312)
0 sites
NotI  (10,300)
1 site		 G C G G C C G C C G C C G G C G
EagI  (10,300)
1 site		 C G G C C G G C C G G C
SpeI  (10,293)
1 site		 A C T A G T T G A T C A
XbaI  (10,207)
1 site		 T C T A G A A G A T C T
BssSI  (9891)
1 site		 C A C G A G G T G C T C
BssSαI  (9891)
1 site		 C A C G A G G T G C T C
RsrII  (9656)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PshAI  (8685)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BbvCI  (8044)
1 site		 C C T C A G C G G A G T C G
PluTI  (7282)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (7280)
1 site		 G G C G C C C C G C G G
NarI  (7279)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (7278)
1 site		 G G C G C C C C G C G G
NdeI  (7274)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
XhoI  (6350)
1 site		 C T C G A G G A G C T C
PspXI  (6350)
1 site		 V C T C G A G B B G A G C T C V
PaeR7I  (6350)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
BsrGI  (5170)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
AatII  (4824)
1 site		 G A C G T C C T G C A G
ZraI  (4822)
1 site		 G A C G T C C T G C A G
BstBI  (4086)
1 site		 T T C G A A A A G C T T
CsiI  (3996)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (3996)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
PasI  (3256)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
MauBI  (3125)
1 site		 C G C G C G C G G C G C G C G C
BssHII  (3125)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
SacI  (1874)
1 site		 G A G C T C C T C G A G
Eco53kI  (1872)
1 site		 G A G C T C C T C G A G
PaqCI  (1785)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
PpuMI  (1632)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
SgrDI  (1402)
1 site		 C G T C G A C G G C A G C T G C
StyI  (1288)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
BstZ17I  (1064)
1 site		 G T A T A C C A T A T G
PmeI  (956)
1 site		 G T T T A A A C C A A A T T T G
MmeI  (762)
1 site		 T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18
Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MfeI  (248)
1 site		 C A A T T G G T T A A C
Start  (0)
0 sites
SL1
21-mer  /  57% GC
1 binding site
10,272 .. 10,292  =  21 annealed bases
Tm  =  59°C
sequencing primer
telL
1 .. 28  =  28 bp
telomeric sequence from coliphage N15
telL
1 .. 28  =  28 bp
telomeric sequence from coliphage N15
TelN
193 .. 2088  =  1896 bp
631 amino acids  =  72.3 kDa
Product: protelomerase of coliphage N15
generates the covalently closed hairpin telomeres
TelN
193 .. 2088  =  1896 bp
631 amino acids  =  72.3 kDa
Product: protelomerase of coliphage N15
generates the covalently closed hairpin telomeres
RepA
5084 .. 9058  =  3975 bp
1324 amino acids  =  149.5 kDa
Product: replication protein of coliphage N15
RepA
5084 .. 9058  =  3975 bp
1324 amino acids  =  149.5 kDa
Product: replication protein of coliphage N15
CB
9307 .. 9915  =  609 bp
202 amino acids  =  22.7 kDa
Product: repressor protein of coliphage N15
CB
9307 .. 9915  =  609 bp
202 amino acids  =  22.7 kDa
Product: repressor protein of coliphage N15
T7Te terminator
10,213 .. 10,240  =  28 bp
phage T7 early transcription terminator
T7Te terminator
10,213 .. 10,240  =  28 bp
phage T7 early transcription terminator
N15 ori
5335 .. 5424  =  90 bp
bidirectional replication origin of coliphage N15
N15 ori
5335 .. 5424  =  90 bp
bidirectional replication origin of coliphage N15
ORF:  193 .. 2088  =  1896 bp
ORF:  631 amino acids  =  72.3 kDa
ORF:  9997 .. 10,311  =  315 bp
ORF:  105 amino acids  =  11.7 kDa
ORF:  1313 .. 1651  =  339 bp
ORF:  112 amino acids  =  12.5 kDa
ORF:  3032 .. 3268  =  237 bp
ORF:  78 amino acids  =  9.0 kDa
ORF:  7082 .. 7555  =  474 bp
ORF:  157 amino acids  =  17.8 kDa
ORF:  2067 .. 2291  =  225 bp
ORF:  74 amino acids  =  8.5 kDa
ORF:  5169 .. 5426  =  258 bp
ORF:  85 amino acids  =  8.7 kDa
ORF:  5466 .. 5786  =  321 bp
ORF:  106 amino acids  =  11.7 kDa
ORF:  6684 .. 6917  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  7317 .. 7544  =  228 bp
ORF:  75 amino acids  =  8.2 kDa
ORF:  8127 .. 8402  =  276 bp
ORF:  91 amino acids  =  10.0 kDa
ORF:  3053 .. 3283  =  231 bp
ORF:  76 amino acids  =  8.4 kDa
ORF:  3812 .. 4252  =  441 bp
ORF:  146 amino acids  =  17.5 kDa
ORF:  5084 .. 9058  =  3975 bp
ORF:  1324 amino acids  =  149.5 kDa
ORF:  1300 .. 1605  =  306 bp
ORF:  101 amino acids  =  11.1 kDa
ORF:  1639 .. 1926  =  288 bp
ORF:  95 amino acids  =  10.9 kDa
ORF:  3280 .. 3582  =  303 bp
ORF:  100 amino acids  =  10.9 kDa
ORF:  4165 .. 4473  =  309 bp
ORF:  102 amino acids  =  11.7 kDa
ORF:  4498 .. 4827  =  330 bp
ORF:  109 amino acids  =  12.6 kDa
ORF:  6439 .. 6696  =  258 bp
ORF:  85 amino acids  =  9.2 kDa
ORF:  9307 .. 9915  =  609 bp
ORF:  202 amino acids  =  22.7 kDa
ORF:  1464 .. 1817  =  354 bp
ORF:  117 amino acids  =  12.3 kDa
ORF:  2262 .. 3056  =  795 bp
ORF:  264 amino acids  =  29.7 kDa
ORF:  4824 .. 5072  =  249 bp
ORF:  82 amino acids  =  9.3 kDa
Click here to try SnapGene

Download pJAZZ-OK GC (left arm).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

Allele TA Vector (linearized)
pCR2.1-TOPO (linearized)
pJAZZ-OK GC (left arm)
pSpark TA
pBAD-TOPO (linearized)
pCR4-TOPO (linearized)
pJAZZ-OK GC (right arm)
pSpark TA Done
pBAD Thio-TOPO (linearized)
pCR8 GW TOPO (linearized)
pLenti6.3 V5-TOPO (linearized)
pSTBlue-1 AccepTor
pBlue-TOPO (linearized)
pCRII-TOPO (linearized)
pLenti7.3 V5-TOPO (linearized)
pT7Blue T-Vector
pcDNA3.1 CT-GFP-TOPO (linearized)
pDrive (linearized)
pLPS-T (linearized)
pTA Plus (linearized)
pcDNA3.1 NT-GFP-TOPO (linearized)
pEF6 V5-His-TOPO (linearized)
pLUG (linearized)
pTargeT (linearized)
pcDNA3.1 V5-His-TOPO (linearized)
pENTR5'-TOPO (linearized)
pLUG-Multi (linearized)
pTOP TA V2 (linearized)
pcDNA3.3-TOPO (linearized)
pET SUMO (linearized)
pMT V5-His-TOPO (linearized)
pTrcHis-TOPO (linearized)
pcDNA3.4-TOPO (linearized)
pETBlue-1 AccepTor
pOptiVEC-TOPO (linearized)
pTrcHis2-TOPO (linearized)
pcDNA4 HisMax-TOPO (linearized)
pEXP5-CT TOPO (linearized)
pPrime (linearized)
pTZ57R_T
pcDNA5 FRT TO-TOPO (linearized)
pEXP5-NT TOPO (linearized)
pQE-30 UA (linearized)
pUC57-T
pcDNA5 FRT V5-His-TOPO (linearized)
pGC Blue (linearized)
pSC-A-amp kan (linearized)
pYES2.1 V5-His-TOPO (linearized)
pcDNA6.2 C-EmGFP-GW TOPO (linearized)
pGEM-T (linearized)
pSecTag FRT V5-His-TOPO (linearized)
RBC T&A Cloning Vector (linearized)
pcDNA6.2 C-YFP-GW TOPO (linearized)
pGEM-T Easy (linearized)
pSMART GC HK (linearized)
T-Vector pMD19 (Simple)
pcDNA6.2 N-EmGFP-GW TOPO (linearized)
pGlow-TOPO (linearized)
pSMART GC LK (linearized)
T-Vector pMD20
pCR-XL-TOPO (linearized)
pIB V5-His-TOPO (linearized)

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free