GeneArt CRISPR Nuclease Vector - CD4 (linearized)

All-in-one CRISPR/Cas9 vector for genome editing, with a CD4 reporter for bead-based cell isolation.

Sequence Author: Thermo Fisher (Invitrogen)

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polIII terminator CMV enhancer ATG SV40 NLS GeneArt® CRISPR Nuclease Vector - CD4 9798 bp
ATG
3202 .. 3204  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
3202 .. 3204  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
V5 tag
3205 .. 3246  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
3205 .. 3246  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
SV40 NLS
3250 .. 3270  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
3250 .. 3270  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Cas9
3295 .. 7395  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
3295 .. 7395  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
7408 .. 7428  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
7408 .. 7428  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
T2A
7450 .. 7503  =  54 bp
18 amino acids  =  1.8 kDa
Product: 2A peptide from Thosea asigna virus capsid protein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
T2A
7450 .. 7503  =  54 bp
18 amino acids  =  1.8 kDa
Product: 2A peptide from Thosea asigna virus capsid protein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
CD4 (truncated)
7504 .. 8775  =  1272 bp
424 amino acids  =  47.0 kDa
2 segments
   Segment 1:  signal peptide  
   7504 .. 7578  =  75 bp
   25 amino acids  =  2.7 kDa
Product: truncated human CD4 cell surface glycoprotein lacking the cytoplasmic tail
CD4 (truncated)
7504 .. 8775  =  1272 bp
424 amino acids  =  47.0 kDa
2 segments
   Segment 2:  
   7579 .. 8775  =  1197 bp
   399 amino acids  =  44.3 kDa
Product: truncated human CD4 cell surface glycoprotein lacking the cytoplasmic tail
CD4 (truncated)
7504 .. 8775  =  1272 bp
424 amino acids  =  47.0 kDa
2 segments
Product: truncated human CD4 cell surface glycoprotein lacking the cytoplasmic tail
AmpR
1287 .. 2147  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1287 .. 2078  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1287 .. 2147  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2079 .. 2147  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1287 .. 2147  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
528 .. 1116  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
528 .. 1116  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
9106 .. 9534  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
9106 .. 9534  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
2518 .. 2897  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2518 .. 2897  =  380 bp
human cytomegalovirus immediate early enhancer
U6 promoter
9554 .. 9802  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
9554 .. 9802  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
CMV promoter
2898 .. 3101  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2898 .. 3101  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
2148 .. 2252  =  105 bp
AmpR promoter
2148 .. 2252  =  105 bp
gRNA scaffold
1 .. 76  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
1 .. 76  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
HSV TK poly(A) signal
8856 .. 8904  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
8856 .. 8904  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
polIII terminator
77 .. 82  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
77 .. 82  =  6 bp
RNA polymerase III transcription terminator
ORF:  1417 .. 1683  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3202 .. 8784  =  5583 bp
ORF:  1860 amino acids  =  212.4 kDa
ORF:  1287 .. 2147  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  5382 .. 5633  =  252 bp
ORF:  83 amino acids  =  9.5 kDa
ORF:  8463 .. 8750  =  288 bp
ORF:  95 amino acids  =  10.4 kDa
ORF:  7799 .. 8053  =  255 bp
ORF:  84 amino acids  =  8.9 kDa
ORF:  3454 .. 5460  =  2007 bp
ORF:  668 amino acids  =  71.1 kDa
ORF:  5791 .. 7083  =  1293 bp
ORF:  430 amino acids  =  46.7 kDa
ORF:  8107 .. 8430  =  324 bp
ORF:  107 amino acids  =  12.0 kDa
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Download GeneArt CRISPR Nuclease Vector - CD4 (linearized).dna file

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