pLVX-hyg-sgRNA1 Vector (Linear)

Linearized lentiviral vector with a hygromycin resistance marker, for cloning and expression of a single guide RNA (sgRNA).

Sequence Author: Clontech (TaKaRa)

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pLVX-hyg-sgRNA1 Vector (Linear) 8219 bp
HygR
644 .. 1669  =  1026 bp
341 amino acids  =  38.0 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
HygR
644 .. 1669  =  1026 bp
341 amino acids  =  38.0 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
AmpR
4413 .. 5273  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4413 .. 5204  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4413 .. 5273  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5205 .. 5273  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4413 .. 5273  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
3' LTR
2493 .. 3126  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
2493 .. 3126  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
5776 .. 6409  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
5776 .. 6409  =  634 bp
3' long terminal repeat (LTR) from HIV-1
WPRE
1697 .. 2285  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
1697 .. 2285  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
3657 .. 4242  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3657 .. 4242  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
PGK promoter
127 .. 626  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
127 .. 626  =  500 bp
mouse phosphoglycerate kinase 1 promoter
U6 promoter
7975 .. 8223  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
7975 .. 8223  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
RRE
7078 .. 7311  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
7078 .. 7311  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
SV40 poly(A) signal
5426 .. 5560  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
5426 .. 5560  =  135 bp
SV40 polyadenylation signal
HIV-1 Ψ
6456 .. 6581  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
6456 .. 6581  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
7803 .. 7918  =  116 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
cPPT/CTS
7803 .. 7918  =  116 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
AmpR promoter
5274 .. 5378  =  105 bp
AmpR promoter
5274 .. 5378  =  105 bp
gRNA scaffold
1 .. 86  =  86 bp
improved guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
1 .. 86  =  86 bp
improved guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
ORF:  4543 .. 4809  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  407 .. 1669  =  1263 bp
ORF:  420 amino acids  =  46.5 kDa
ORF:  1787 .. 2314  =  528 bp
ORF:  175 amino acids  =  19.0 kDa
ORF:  6956 .. 7807  =  852 bp
ORF:  283 amino acids  =  32.7 kDa
ORF:  1800 .. 2096  =  297 bp
ORF:  98 amino acids  =  10.2 kDa
ORF:  6790 .. 7251  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
ORF:  4413 .. 5273  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  572 .. 1402  =  831 bp
ORF:  276 amino acids  =  31.0 kDa
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Download pLVX-hyg-sgRNA1 Vector (Linear).dna file

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