pCLIP-gRNA-EFS-ZsGreen

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
polIII terminator BsmBI (2505) BsmBI (4390) pCLIP-gRNA-EFS-ZsGreen 8762 bp
BsmBI  (2505)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
BsmBI  (4390)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Stuffer
2506 .. 4390  =  1885 bp
Stuffer
2506 .. 4390  =  1885 bp
AmpR
7767 .. 8627  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   7767 .. 8558  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7767 .. 8627  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   8559 .. 8627  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7767 .. 8627  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ZsGreen1
4759 .. 5454  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ZsGreen1
4759 .. 5454  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
WPRE
5468 .. 6056  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
5468 .. 6056  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
7011 .. 7596  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7011 .. 7596  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
27 .. 404  =  378 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
27 .. 404  =  378 bp
human cytomegalovirus immediate early enhancer
SV40
6593 .. 6922  =  330 bp
SV40 enhancer and early promoter
SV40
6593 .. 6922  =  330 bp
SV40 enhancer and early promoter
5' LTR (truncated)
411 .. 668  =  258 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
411 .. 668  =  258 bp
truncated 5' long terminal repeat (LTR) from HIV-1
RRE
1333 .. 1566  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1333 .. 1566  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
3' LTR (ΔU3)
6264 .. 6497  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
3' LTR (ΔU3)
6264 .. 6497  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
EF-1α core promoter
4528 .. 4739  =  212 bp
core promoter for human elongation factor EF-1α
EF-1α core promoter
4528 .. 4739  =  212 bp
core promoter for human elongation factor EF-1α
HIV-1 Ψ
715 .. 840  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
715 .. 840  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
2093 .. 2210  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
2093 .. 2210  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
8628 .. 8732  =  105 bp
AmpR promoter
8628 .. 8732  =  105 bp
gRNA scaffold
4391 .. 4466  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
4391 .. 4466  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
polIII terminator
4467 .. 4472  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
4467 .. 4472  =  6 bp
RNA polymerase III transcription terminator
U6 promoter
2261 .. 2509  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
2261 .. 2509  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
ORF:  4759 .. 5454  =  696 bp
ORF:  231 amino acids  =  26.1 kDa
ORF:  7897 .. 8163  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1211 .. 2098  =  888 bp
ORF:  295 amino acids  =  33.8 kDa
ORF:  5558 .. 6085  =  528 bp
ORF:  175 amino acids  =  19.0 kDa
ORF:  5571 .. 5867  =  297 bp
ORF:  98 amino acids  =  10.2 kDa
ORF:  4425 .. 4859  =  435 bp
ORF:  144 amino acids  =  15.6 kDa
ORF:  5136 .. 5429  =  294 bp
ORF:  97 amino acids  =  10.9 kDa
ORF:  7767 .. 8627  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  8524 .. 31  =  270 bp
ORF:  89 amino acids  =  10.4 kDa
ORF:  2684 .. 3079  =  396 bp
ORF:  131 amino acids  =  14.0 kDa
ORF:  1126 .. 1506  =  381 bp
ORF:  126 amino acids  =  13.3 kDa
Click here to try SnapGene

Download pCLIP-gRNA-EFS-ZsGreen.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.