lentiGuide-Puro

Zhang lab lentiviral vector, also known as pLentiGuide-Puro, for expressing a single guide RNA (sgRNA) in Cas9-expressing cells. Use together with lentiCas9-Blast.

Sequence Author: Zhang Lab / Addgene #52963

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
BsmBI (9882) BsmBI (7997) polIII terminator lentiGuide-Puro 10,183 bp
BsmBI  (9882)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
BsmBI  (7997)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
filler
7998 .. 9882  =  1885 bp
digest with BsmBI to replace the filler with an sgRNA
filler
7998 .. 9882  =  1885 bp
digest with BsmBI to replace the filler with an sgRNA
EF-1α promoter
87 .. 1265  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
EF-1α promoter
87 .. 1265  =  1179 bp
strong constitutive promoter for human elongation factor EF-1α
AmpR
3953 .. 4813  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3953 .. 4021  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3953 .. 4813  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4022 .. 4813  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3953 .. 4813  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
PuroR
1278 .. 1877  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
1278 .. 1877  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
WPRE
1893 .. 2481  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
1893 .. 2481  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
4984 .. 5572  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4984 .. 5572  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
3367 .. 3822  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
3367 .. 3822  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
3' LTR (ΔU3)
2553 .. 2786  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
3' LTR (ΔU3)
2553 .. 2786  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
RRE
7113 .. 7346  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
7113 .. 7346  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RSV promoter
6041 .. 6267  =  227 bp
Rous sarcoma virus enhancer/promoter
RSV promoter
6041 .. 6267  =  227 bp
Rous sarcoma virus enhancer/promoter
5' LTR (truncated)
6268 .. 6448  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
6268 .. 6448  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
SV40 ori
3025 .. 3160  =  136 bp
SV40 origin of replication
SV40 ori
3025 .. 3160  =  136 bp
SV40 origin of replication
HIV-1 Ψ
6495 .. 6620  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
6495 .. 6620  =  126 bp
packaging signal of human immunodeficiency virus type 1
SV40 poly(A) signal
2864 .. 2985  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2864 .. 2985  =  122 bp
SV40 polyadenylation signal
cPPT/CTS
10,014 .. 10,131  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
10,014 .. 10,131  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
3848 .. 3952  =  105 bp
AmpR promoter
3848 .. 3952  =  105 bp
gRNA scaffold
9883 .. 9958  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
9883 .. 9958  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
polIII terminator
9959 .. 9964  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
9959 .. 9964  =  6 bp
RNA polymerase III transcription terminator
EF-1α intron A
318 .. 1256  =  939 bp
intron upstream of the start codon of human EF-1α
EF-1α intron A
318 .. 1256  =  939 bp
intron upstream of the start codon of human EF-1α
U6 promoter
7753 .. 8001  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
7753 .. 8001  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
ORF:  643 .. 1080  =  438 bp
ORF:  145 amino acids  =  15.9 kDa
ORF:  1996 .. 2526  =  531 bp
ORF:  176 amino acids  =  18.7 kDa
ORF:  6991 .. 7866  =  876 bp
ORF:  291 amino acids  =  33.6 kDa
ORF:  722 .. 955  =  234 bp
ORF:  77 amino acids  =  7.7 kDa
ORF:  3953 .. 4813  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1278 .. 1877  =  600 bp
ORF:  199 amino acids  =  21.5 kDa
ORF:  1983 .. 2492  =  510 bp
ORF:  169 amino acids  =  18.5 kDa
ORF:  2886 .. 3122  =  237 bp
ORF:  78 amino acids  =  8.6 kDa
ORF:  5970 .. 6215  =  246 bp
ORF:  81 amino acids  =  8.9 kDa
ORF:  5897 .. 6196  =  300 bp
ORF:  99 amino acids  =  10.9 kDa
ORF:  385 .. 1005  =  621 bp
ORF:  206 amino acids  =  23.0 kDa
ORF:  4417 .. 4683  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  8176 .. 8571  =  396 bp
ORF:  131 amino acids  =  14.0 kDa
ORF:  6906 .. 7286  =  381 bp
ORF:  126 amino acids  =  13.3 kDa
Click here to try SnapGene

Download lentiGuide-Puro.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.