pGuide-it-sgRNA1 Vector (Linear)

Linearized CRISPR/Cas9 vector with an improved scaffold, for cloning and expression of a single guide RNA (sgRNA).

Sequence Author: Clontech (TaKaRa)

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Guide-it™ Sequencing Primer 1 (2943 .. 2966) pGuide-it-sgRNA1 Vector (Linear) 3020 bp
Guide-it™ Sequencing Primer 1
24-mer  /  38% GC
1 binding site
2943 .. 2966  =  24 annealed bases
Tm  =  56°C
AmpR
1276 .. 2136  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1276 .. 2067  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1276 .. 2136  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2068 .. 2136  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1276 .. 2136  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
517 .. 1105  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
517 .. 1105  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
U6 promoter
2776 .. 3024  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
2776 .. 3024  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
AmpR promoter
2137 .. 2241  =  105 bp
AmpR promoter
2137 .. 2241  =  105 bp
gRNA scaffold
1 .. 86  =  86 bp
improved guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
1 .. 86  =  86 bp
improved guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
ORF:  1406 .. 1672  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2522 .. 2746  =  225 bp
ORF:  74 amino acids  =  8.8 kDa
ORF:  1276 .. 2136  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Download pGuide-it-sgRNA1 Vector (Linear).dna file

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Individual Sequences & Maps

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