pSECC

Vector for expressing a single guide RNA (sgRNA) of interest together with Cas9 and Cre recombinase.
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Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
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AmpR promoter SV40 NLS BsmBI (2899) BsmBI (4784) gRNA scaffold polIII terminator ATG FLAG SV40 NLS pSECC 13,895 bp
BsmBI  (2899)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
BsmBI  (4784)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
ATG
5380 .. 5382  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
5380 .. 5382  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
FLAG
5383 .. 5406  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
5383 .. 5406  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
SV40 NLS
5413 .. 5433  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 (simian virus 40) large T antigen
SV40 NLS
5413 .. 5433  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 (simian virus 40) large T antigen
Cas9
5458 .. 9558  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
5458 .. 9558  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
nucleoplasmin NLS
9559 .. 9606  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
nucleoplasmin NLS
9559 .. 9606  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
P2A
9622 .. 9678  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1 polyprotein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
P2A
9622 .. 9678  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1 polyprotein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
SV40 NLS
9682 .. 9702  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 (simian virus 40) large T antigen
SV40 NLS
9682 .. 9702  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 (simian virus 40) large T antigen
Cre
9703 .. 10,731  =  1029 bp
342 amino acids  =  38.4 kDa
Product: site-specific recombinase
Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences.
Cre
9703 .. 10,731  =  1029 bp
342 amino acids  =  38.4 kDa
Product: site-specific recombinase
Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences.
Filler
2900 .. 4784  =  1885 bp
Filler
2900 .. 4784  =  1885 bp
AmpR
12,902 .. 13,762  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   12,902 .. 13,693  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
12,902 .. 13,762  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   13,694 .. 13,762  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
12,902 .. 13,762  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
12,143 .. 12,731  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
12,143 .. 12,731  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
WPRE
10,784 .. 11,372  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
10,784 .. 11,372  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
CMV enhancer
237 .. 616  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
237 .. 616  =  380 bp
human cytomegalovirus immediate early enhancer
RRE
1685 .. 1918  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1685 .. 1918  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
EF-1α core promoter
5124 .. 5335  =  212 bp
core promoter for human elongation factor EF-1α
EF-1α core promoter
5124 .. 5335  =  212 bp
core promoter for human elongation factor EF-1α
CMV promoter
618 .. 816  =  199 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
618 .. 816  =  199 bp
human cytomegalovirus (CMV) immediate early promoter
5' LTR (truncated)
11,901 .. 12,081  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
11,901 .. 12,081  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
834 .. 1014  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
834 .. 1014  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
HIV-1 Ψ
1061 .. 1186  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
1061 .. 1186  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
4908 .. 5025  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
4908 .. 5025  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
2445 .. 2562  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
2445 .. 2562  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
13,763 .. 13,867  =  105 bp
AmpR promoter
13,763 .. 13,867  =  105 bp
gRNA scaffold
4785 .. 4860  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
4785 .. 4860  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
polIII terminator
4861 .. 4866  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
4861 .. 4866  =  6 bp
RNA polymerase III transcription terminator
U6 promoter
2655 .. 2903  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
2655 .. 2903  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
C-to-A
10,380 .. 10,380  =  1 bp
synonymous mutation to eliminate the BsmBI site in the Cre gene
C-to-A
10,380 .. 10,380  =  1 bp
synonymous mutation to eliminate the BsmBI site in the Cre gene
ORF:  5380 .. 10,731  =  5352 bp
ORF:  1783 amino acids  =  204.6 kDa
ORF:  10,874 .. 11,428  =  555 bp
ORF:  184 amino acids  =  20.5 kDa
ORF:  1563 .. 2450  =  888 bp
ORF:  295 amino acids  =  33.8 kDa
ORF:  10,887 .. 11,927  =  1041 bp
ORF:  346 amino acids  =  38.3 kDa
ORF:  7368 .. 7817  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
ORF:  12,902 .. 13,762  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  5365 .. 7974  =  2610 bp
ORF:  869 amino acids  =  91.6 kDa
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Download pSECC.dna file

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